Peptide-functionalized quantum dots for potential applications in the imaging and treatment of obesity

Ntevheleni Thovhogi,1 Nicole Remaliah Samantha Sibuyi,1 Martin Opiyo Onani,2 Mervin Meyer,1 Abram Madimabe Madiehe1 1Department of Science and Technology (DST)/Mintek Nanotechnology Innovation Centre, Biolabels Unit, Department of Biotechnology, 2Organometallics and Nanomaterials, Department of Che...

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Autores principales:	Thovhogi N, Sibuyi NRS, Onani MO, Meyer M, Madiehe AM
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2018
Materias:	Adipose Homing Peptide Drug delivery Nanotechnology Prohibitin White adipose tissue Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/a98bfd066aba444c81d0100f5bb323ae
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spelling	oai:doaj.org-article:a98bfd066aba444c81d0100f5bb323ae2021-12-02T06:00:45ZPeptide-functionalized quantum dots for potential applications in the imaging and treatment of obesity1178-2013https://doaj.org/article/a98bfd066aba444c81d0100f5bb323ae2018-04-01T00:00:00Zhttps://www.dovepress.com/peptide-functionalized-quantum-dots-for-potential-applications-in-the--peer-reviewed-article-IJNhttps://doaj.org/toc/1178-2013Ntevheleni Thovhogi,1 Nicole Remaliah Samantha Sibuyi,1 Martin Opiyo Onani,2 Mervin Meyer,1 Abram Madimabe Madiehe1 1Department of Science and Technology (DST)/Mintek Nanotechnology Innovation Centre, Biolabels Unit, Department of Biotechnology, 2Organometallics and Nanomaterials, Department of Chemistry, University of the Western Cape, Bellville, South Africa Background: Obesity is a worldwide epidemic affecting millions of people. The current pharmacological treatment of obesity remains limited and ineffective due to drugs’ undesirable side effects. Hence, there is a need for novel or improved strategies for long-term therapies that will help prevent the disease progression into other chronic diseases. Nanotechnology holds the future for the treatment of obesity because of its versatility, as shown by improved drug efficiency and safety in cancer clinical trials. Nano-based drug delivery systems could potentially do the same for obesity through targeted drug delivery. This study investigated the use of peptide-functionalized quantum dots (QDs) for the imaging of prohibitin (PHB)-expressing cells in vitro and in diet-induced obese rats, which could potentially be used as nanocarriers of antiobesity drugs. Methods: Cadmium (Cd)-based QDs were functionalized with an adipose homing peptide (AHP) and injected intravenously into lean and obese Wistar rats. Biodistribution of the QDs was analyzed by an IVIS® Lumina XR imaging system and inductively coupled plasma optical emission spectroscopy (ICP-OES). For in vitro studies, PHB-expressing (Caco-2 and MCF-7) and non-PHB-expressing (KMST-6 and CHO) cells were exposed to either unfunctionalized QDs (QD625) or AHP-functionalized QDs (AHP-QD625) and analyzed by fluorescence microscopy. Results: AHP-QD625 accumulated significantly in PHB-expressing cells in vitro when compared with non-PHB-expressing cells. In vivo data indicated that QD625 accumulated mainly in the reticuloendothelial system (RES) organs, while the AHP-QD625 accumulated mostly in the white adipose tissues (WATs). Conclusion: AHP-functionalized QDs were successfully and selectively delivered to the PHB-expressing cells in vitro (Caco-2 and MCF-7 cells) and in the WAT vasculature in vivo. This nanotechnology-based approach could potentially be used for dual targeted drug delivery and molecular imaging of adipose tissues in obese patients in real time. Keywords: adipose homing peptide, drug delivery, nanotechnology, prohibitin, white adipose tissueThovhogi NSibuyi NRSOnani MOMeyer MMadiehe AMDove Medical PressarticleAdipose Homing PeptideDrug deliveryNanotechnologyProhibitinWhite adipose tissueMedicine (General)R5-920ENInternational Journal of Nanomedicine, Vol Volume 13, Pp 2551-2559 (2018)
institution	DOAJ
collection	DOAJ
language	EN
topic	Adipose Homing Peptide Drug delivery Nanotechnology Prohibitin White adipose tissue Medicine (General) R5-920
spellingShingle	Adipose Homing Peptide Drug delivery Nanotechnology Prohibitin White adipose tissue Medicine (General) R5-920 Thovhogi N Sibuyi NRS Onani MO Meyer M Madiehe AM Peptide-functionalized quantum dots for potential applications in the imaging and treatment of obesity
description	Ntevheleni Thovhogi,1 Nicole Remaliah Samantha Sibuyi,1 Martin Opiyo Onani,2 Mervin Meyer,1 Abram Madimabe Madiehe1 1Department of Science and Technology (DST)/Mintek Nanotechnology Innovation Centre, Biolabels Unit, Department of Biotechnology, 2Organometallics and Nanomaterials, Department of Chemistry, University of the Western Cape, Bellville, South Africa Background: Obesity is a worldwide epidemic affecting millions of people. The current pharmacological treatment of obesity remains limited and ineffective due to drugs’ undesirable side effects. Hence, there is a need for novel or improved strategies for long-term therapies that will help prevent the disease progression into other chronic diseases. Nanotechnology holds the future for the treatment of obesity because of its versatility, as shown by improved drug efficiency and safety in cancer clinical trials. Nano-based drug delivery systems could potentially do the same for obesity through targeted drug delivery. This study investigated the use of peptide-functionalized quantum dots (QDs) for the imaging of prohibitin (PHB)-expressing cells in vitro and in diet-induced obese rats, which could potentially be used as nanocarriers of antiobesity drugs. Methods: Cadmium (Cd)-based QDs were functionalized with an adipose homing peptide (AHP) and injected intravenously into lean and obese Wistar rats. Biodistribution of the QDs was analyzed by an IVIS® Lumina XR imaging system and inductively coupled plasma optical emission spectroscopy (ICP-OES). For in vitro studies, PHB-expressing (Caco-2 and MCF-7) and non-PHB-expressing (KMST-6 and CHO) cells were exposed to either unfunctionalized QDs (QD625) or AHP-functionalized QDs (AHP-QD625) and analyzed by fluorescence microscopy. Results: AHP-QD625 accumulated significantly in PHB-expressing cells in vitro when compared with non-PHB-expressing cells. In vivo data indicated that QD625 accumulated mainly in the reticuloendothelial system (RES) organs, while the AHP-QD625 accumulated mostly in the white adipose tissues (WATs). Conclusion: AHP-functionalized QDs were successfully and selectively delivered to the PHB-expressing cells in vitro (Caco-2 and MCF-7 cells) and in the WAT vasculature in vivo. This nanotechnology-based approach could potentially be used for dual targeted drug delivery and molecular imaging of adipose tissues in obese patients in real time. Keywords: adipose homing peptide, drug delivery, nanotechnology, prohibitin, white adipose tissue
format	article
author	Thovhogi N Sibuyi NRS Onani MO Meyer M Madiehe AM
author_facet	Thovhogi N Sibuyi NRS Onani MO Meyer M Madiehe AM
author_sort	Thovhogi N
title	Peptide-functionalized quantum dots for potential applications in the imaging and treatment of obesity
title_short	Peptide-functionalized quantum dots for potential applications in the imaging and treatment of obesity
title_full	Peptide-functionalized quantum dots for potential applications in the imaging and treatment of obesity
title_fullStr	Peptide-functionalized quantum dots for potential applications in the imaging and treatment of obesity
title_full_unstemmed	Peptide-functionalized quantum dots for potential applications in the imaging and treatment of obesity
title_sort	peptide-functionalized quantum dots for potential applications in the imaging and treatment of obesity
publisher	Dove Medical Press
publishDate	2018
url	https://doaj.org/article/a98bfd066aba444c81d0100f5bb323ae
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_version_	1718400127565037568

Peptide-functionalized quantum dots for potential applications in the imaging and treatment of obesity

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