The paracrine effects of adipocytes on lipid metabolism in doxorubicin-treated triple negative breast cancer cells

Adipocytes in the breast tumour microenvironment promotes acquired treatment resistance. We used an in vitro adipocyte-conditioned media approach to investigate the direct paracrine effects of adipocyte secretory factors on MDA-MB-231 breast cancer cells treated with doxorubicin to clarify the under...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Ilze Mentoor, Anna-Mart Engelbrecht, Mari van de Vyver, Paul J. van Jaarsveld, Theo Nell
Formato:	article
Lenguaje:	EN
Publicado:	Taylor & Francis Group 2021
Materias:	adipocytes breast cancer lipolysis fatty acids inflammation treatment resistance Diseases of the endocrine glands. Clinical endocrinology RC648-665 Cytology QH573-671 Physiology QP1-981
Acceso en línea:	https://doaj.org/article/a98c646362144cd38b04f51a2ee5f93f
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:a98c646362144cd38b04f51a2ee5f93f
record_format	dspace
spelling	oai:doaj.org-article:a98c646362144cd38b04f51a2ee5f93f2021-11-26T11:19:49ZThe paracrine effects of adipocytes on lipid metabolism in doxorubicin-treated triple negative breast cancer cells2162-39452162-397X10.1080/21623945.2021.1979758https://doaj.org/article/a98c646362144cd38b04f51a2ee5f93f2021-01-01T00:00:00Zhttp://dx.doi.org/10.1080/21623945.2021.1979758https://doaj.org/toc/2162-3945https://doaj.org/toc/2162-397XAdipocytes in the breast tumour microenvironment promotes acquired treatment resistance. We used an in vitro adipocyte-conditioned media approach to investigate the direct paracrine effects of adipocyte secretory factors on MDA-MB-231 breast cancer cells treated with doxorubicin to clarify the underlying treatment resistance mechanisms. Cell-viability assays, and Western blots were performed to determine alterations in apoptotic, proliferation and lipid metabolism protein markers. Free fatty acids (FFA) and inflammatory markers in the collected treatment-conditioned media were also quantified. Adipocyte secretory factors increased the cell-viability of doxorubicin-treated cells (p < 0.0001), which did not correspond to apoptosis or proliferation pathways. Adipocyte secretory factors increased the protein expression of hormone-sensitive lipase (p < 0.05) in doxorubicin-treated cells. Adipocyte secretory factors increased the utilization of leptin (p < 0.05) and MCP-1 (p < 0.01) proteins and possibly inhibited release of linoleic acid by doxorubicin-treated cells (treatment-conditioned media FFA profiles). Adipocyte secretory factors induced doxorubicin treatment resistance, by increasing the utilization of inflammatory mediators and inhibiting the release of FFA by doxorubicin-treated cells. This further promotes inflammation and lipid metabolic reprogramming (lipid storage) in the tumour microenvironment, which breast cancer cells use to evade the toxic effects induced by doxorubicin and confers to acquired treatment resistance.Ilze MentoorAnna-Mart EngelbrechtMari van de VyverPaul J. van JaarsveldTheo NellTaylor & Francis Grouparticleadipocytesbreast cancerlipolysisfatty acidsinflammationtreatment resistanceDiseases of the endocrine glands. Clinical endocrinologyRC648-665CytologyQH573-671PhysiologyQP1-981ENAdipocyte, Vol 10, Iss 1, Pp 505-523 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	adipocytes breast cancer lipolysis fatty acids inflammation treatment resistance Diseases of the endocrine glands. Clinical endocrinology RC648-665 Cytology QH573-671 Physiology QP1-981
spellingShingle	adipocytes breast cancer lipolysis fatty acids inflammation treatment resistance Diseases of the endocrine glands. Clinical endocrinology RC648-665 Cytology QH573-671 Physiology QP1-981 Ilze Mentoor Anna-Mart Engelbrecht Mari van de Vyver Paul J. van Jaarsveld Theo Nell The paracrine effects of adipocytes on lipid metabolism in doxorubicin-treated triple negative breast cancer cells
description	Adipocytes in the breast tumour microenvironment promotes acquired treatment resistance. We used an in vitro adipocyte-conditioned media approach to investigate the direct paracrine effects of adipocyte secretory factors on MDA-MB-231 breast cancer cells treated with doxorubicin to clarify the underlying treatment resistance mechanisms. Cell-viability assays, and Western blots were performed to determine alterations in apoptotic, proliferation and lipid metabolism protein markers. Free fatty acids (FFA) and inflammatory markers in the collected treatment-conditioned media were also quantified. Adipocyte secretory factors increased the cell-viability of doxorubicin-treated cells (p < 0.0001), which did not correspond to apoptosis or proliferation pathways. Adipocyte secretory factors increased the protein expression of hormone-sensitive lipase (p < 0.05) in doxorubicin-treated cells. Adipocyte secretory factors increased the utilization of leptin (p < 0.05) and MCP-1 (p < 0.01) proteins and possibly inhibited release of linoleic acid by doxorubicin-treated cells (treatment-conditioned media FFA profiles). Adipocyte secretory factors induced doxorubicin treatment resistance, by increasing the utilization of inflammatory mediators and inhibiting the release of FFA by doxorubicin-treated cells. This further promotes inflammation and lipid metabolic reprogramming (lipid storage) in the tumour microenvironment, which breast cancer cells use to evade the toxic effects induced by doxorubicin and confers to acquired treatment resistance.
format	article
author	Ilze Mentoor Anna-Mart Engelbrecht Mari van de Vyver Paul J. van Jaarsveld Theo Nell
author_facet	Ilze Mentoor Anna-Mart Engelbrecht Mari van de Vyver Paul J. van Jaarsveld Theo Nell
author_sort	Ilze Mentoor
title	The paracrine effects of adipocytes on lipid metabolism in doxorubicin-treated triple negative breast cancer cells
title_short	The paracrine effects of adipocytes on lipid metabolism in doxorubicin-treated triple negative breast cancer cells
title_full	The paracrine effects of adipocytes on lipid metabolism in doxorubicin-treated triple negative breast cancer cells
title_fullStr	The paracrine effects of adipocytes on lipid metabolism in doxorubicin-treated triple negative breast cancer cells
title_full_unstemmed	The paracrine effects of adipocytes on lipid metabolism in doxorubicin-treated triple negative breast cancer cells
title_sort	paracrine effects of adipocytes on lipid metabolism in doxorubicin-treated triple negative breast cancer cells
publisher	Taylor & Francis Group
publishDate	2021
url	https://doaj.org/article/a98c646362144cd38b04f51a2ee5f93f
work_keys_str_mv	AT ilzementoor theparacrineeffectsofadipocytesonlipidmetabolismindoxorubicintreatedtriplenegativebreastcancercells AT annamartengelbrecht theparacrineeffectsofadipocytesonlipidmetabolismindoxorubicintreatedtriplenegativebreastcancercells AT marivandevyver theparacrineeffectsofadipocytesonlipidmetabolismindoxorubicintreatedtriplenegativebreastcancercells AT pauljvanjaarsveld theparacrineeffectsofadipocytesonlipidmetabolismindoxorubicintreatedtriplenegativebreastcancercells AT theonell theparacrineeffectsofadipocytesonlipidmetabolismindoxorubicintreatedtriplenegativebreastcancercells AT ilzementoor paracrineeffectsofadipocytesonlipidmetabolismindoxorubicintreatedtriplenegativebreastcancercells AT annamartengelbrecht paracrineeffectsofadipocytesonlipidmetabolismindoxorubicintreatedtriplenegativebreastcancercells AT marivandevyver paracrineeffectsofadipocytesonlipidmetabolismindoxorubicintreatedtriplenegativebreastcancercells AT pauljvanjaarsveld paracrineeffectsofadipocytesonlipidmetabolismindoxorubicintreatedtriplenegativebreastcancercells AT theonell paracrineeffectsofadipocytesonlipidmetabolismindoxorubicintreatedtriplenegativebreastcancercells
_version_	1718409492229521408

The paracrine effects of adipocytes on lipid metabolism in doxorubicin-treated triple negative breast cancer cells

Ejemplares similares