Helminth infection induces non-functional sensitization to house dust mites.

<h4>Background</h4>IgE characterizes the humoral response of allergic sensitization but less is known about what modulates its function and why some patients present clinical symptoms for a given IgE level and others do not. An IgE response also occurs during helminth diseases, independe...

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Autores principales:	Virginie Doyen, Carine Truyens, Hoa Nhu Thi, Hiep Tran Thi Mong, Thanh Le Chi, Frederic De Blay, Phuong Thi Ngoe Huynh, Olivier Michel, Francis Corazza
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/a98f594732ec4e229fe3f05cfb6b5e39
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spelling	oai:doaj.org-article:a98f594732ec4e229fe3f05cfb6b5e392021-12-02T20:09:48ZHelminth infection induces non-functional sensitization to house dust mites.1932-620310.1371/journal.pone.0253887https://doaj.org/article/a98f594732ec4e229fe3f05cfb6b5e392021-01-01T00:00:00Zhttps://doi.org/10.1371/journal.pone.0253887https://doaj.org/toc/1932-6203<h4>Background</h4>IgE characterizes the humoral response of allergic sensitization but less is known about what modulates its function and why some patients present clinical symptoms for a given IgE level and others do not. An IgE response also occurs during helminth diseases, independently of allergic symptoms. This response could be a model of non-functional IgE.<h4>Objective</h4>To study the IgE response against environmental allergens induced during natural helminth infection.<h4>Methods</h4>In 28 non allergic subjects from the periphery of Ho Chi Minh city with (H+, n = 18) and without helminth infection (H-, n = 10), we measured IgE and IgG4 against several components of Dermatophagoïdes pteronyssinus (Dpt) and Ascaris (a marker of immunization against nematodes), and determined the IgE component sensitization profile using microarray ISAC biochips. The functional ability of IgE to induce degranulation of cultured mast cells was evaluated in the presence of Dpt.<h4>Results</h4>Non allergic H+ subjects exhibited higher levels of IgE against Dpt compared to H- subjects. Dpt IgE were not functional in vitro and did not recognize usual Dpt major allergens. IgE recognized other component allergens that belong to different protein families, and most were glycosylated. Depletion of IgE recognizing carbohydrate cross-reactive determinant (CCD) did not induce a reduction in Dpt IgE. The Dpt IgG4 were not significantly different.<h4>Conclusion</h4>Helminth infections induced IgE against allergens such as Dpt and molecular components that belong to different sources as well as against CCD (such as β-1,2-xylose and/or ⍺-1,3-fucose substituted N-glycans). Dpt IgE were not able to induce degranulation of mast cells and were not explained by sensitization to usual major allergens or N-glycans.Virginie DoyenCarine TruyensHoa Nhu ThiHiep Tran Thi MongThanh Le ChiFrederic De BlayPhuong Thi Ngoe HuynhOlivier MichelFrancis CorazzaPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 16, Iss 7, p e0253887 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Virginie Doyen Carine Truyens Hoa Nhu Thi Hiep Tran Thi Mong Thanh Le Chi Frederic De Blay Phuong Thi Ngoe Huynh Olivier Michel Francis Corazza Helminth infection induces non-functional sensitization to house dust mites.
description	<h4>Background</h4>IgE characterizes the humoral response of allergic sensitization but less is known about what modulates its function and why some patients present clinical symptoms for a given IgE level and others do not. An IgE response also occurs during helminth diseases, independently of allergic symptoms. This response could be a model of non-functional IgE.<h4>Objective</h4>To study the IgE response against environmental allergens induced during natural helminth infection.<h4>Methods</h4>In 28 non allergic subjects from the periphery of Ho Chi Minh city with (H+, n = 18) and without helminth infection (H-, n = 10), we measured IgE and IgG4 against several components of Dermatophagoïdes pteronyssinus (Dpt) and Ascaris (a marker of immunization against nematodes), and determined the IgE component sensitization profile using microarray ISAC biochips. The functional ability of IgE to induce degranulation of cultured mast cells was evaluated in the presence of Dpt.<h4>Results</h4>Non allergic H+ subjects exhibited higher levels of IgE against Dpt compared to H- subjects. Dpt IgE were not functional in vitro and did not recognize usual Dpt major allergens. IgE recognized other component allergens that belong to different protein families, and most were glycosylated. Depletion of IgE recognizing carbohydrate cross-reactive determinant (CCD) did not induce a reduction in Dpt IgE. The Dpt IgG4 were not significantly different.<h4>Conclusion</h4>Helminth infections induced IgE against allergens such as Dpt and molecular components that belong to different sources as well as against CCD (such as β-1,2-xylose and/or ⍺-1,3-fucose substituted N-glycans). Dpt IgE were not able to induce degranulation of mast cells and were not explained by sensitization to usual major allergens or N-glycans.
format	article
author	Virginie Doyen Carine Truyens Hoa Nhu Thi Hiep Tran Thi Mong Thanh Le Chi Frederic De Blay Phuong Thi Ngoe Huynh Olivier Michel Francis Corazza
author_facet	Virginie Doyen Carine Truyens Hoa Nhu Thi Hiep Tran Thi Mong Thanh Le Chi Frederic De Blay Phuong Thi Ngoe Huynh Olivier Michel Francis Corazza
author_sort	Virginie Doyen
title	Helminth infection induces non-functional sensitization to house dust mites.
title_short	Helminth infection induces non-functional sensitization to house dust mites.
title_full	Helminth infection induces non-functional sensitization to house dust mites.
title_fullStr	Helminth infection induces non-functional sensitization to house dust mites.
title_full_unstemmed	Helminth infection induces non-functional sensitization to house dust mites.
title_sort	helminth infection induces non-functional sensitization to house dust mites.
publisher	Public Library of Science (PLoS)
publishDate	2021
url	https://doaj.org/article/a98f594732ec4e229fe3f05cfb6b5e39
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Helminth infection induces non-functional sensitization to house dust mites.

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