Identifying and characterizing SCRaMbLEd synthetic yeast using ReSCuES

Identifying and characterizing SCRaMbLEd synthetic yeast using ReSCuES

The use of synthetic chromosomes and the recombinase-based SCRaMbLE system could enable rapid strain evolution through massive chromosome rearrangements. Here the authors present ReSCuES, which uses auxotrophic markers to rapidly identify yeast with rearrangements for strain engineering.

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Detalles Bibliográficos
Autores principales: Zhouqing Luo, Lihui Wang, Yun Wang, Weimin Zhang, Yakun Guo, Yue Shen, Linghuo Jiang, Qingyu Wu, Chong Zhang, Yizhi Cai, Junbiao Dai
Formato: article
Lenguaje:EN
Publicado: Nature Portfolio 2018
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Science
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Acceso en línea:https://doaj.org/article/a9907f577d24483dabdd7564338c8e60
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Sumario:The use of synthetic chromosomes and the recombinase-based SCRaMbLE system could enable rapid strain evolution through massive chromosome rearrangements. Here the authors present ReSCuES, which uses auxotrophic markers to rapidly identify yeast with rearrangements for strain engineering.

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