Identifying and characterizing SCRaMbLEd synthetic yeast using ReSCuES

The use of synthetic chromosomes and the recombinase-based SCRaMbLE system could enable rapid strain evolution through massive chromosome rearrangements. Here the authors present ReSCuES, which uses auxotrophic markers to rapidly identify yeast with rearrangements for strain engineering.

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Autores principales: Zhouqing Luo, Lihui Wang, Yun Wang, Weimin Zhang, Yakun Guo, Yue Shen, Linghuo Jiang, Qingyu Wu, Chong Zhang, Yizhi Cai, Junbiao Dai
Formato: article
Lenguaje:EN
Publicado: Nature Portfolio 2018
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Acceso en línea:https://doaj.org/article/a9907f577d24483dabdd7564338c8e60
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spelling oai:doaj.org-article:a9907f577d24483dabdd7564338c8e602021-12-02T14:39:08ZIdentifying and characterizing SCRaMbLEd synthetic yeast using ReSCuES10.1038/s41467-017-00806-y2041-1723https://doaj.org/article/a9907f577d24483dabdd7564338c8e602018-05-01T00:00:00Zhttps://doi.org/10.1038/s41467-017-00806-yhttps://doaj.org/toc/2041-1723The use of synthetic chromosomes and the recombinase-based SCRaMbLE system could enable rapid strain evolution through massive chromosome rearrangements. Here the authors present ReSCuES, which uses auxotrophic markers to rapidly identify yeast with rearrangements for strain engineering.Zhouqing LuoLihui WangYun WangWeimin ZhangYakun GuoYue ShenLinghuo JiangQingyu WuChong ZhangYizhi CaiJunbiao DaiNature PortfolioarticleScienceQENNature Communications, Vol 9, Iss 1, Pp 1-10 (2018)
institution DOAJ
collection DOAJ
language EN
topic Science
Q
spellingShingle Science
Q
Zhouqing Luo
Lihui Wang
Yun Wang
Weimin Zhang
Yakun Guo
Yue Shen
Linghuo Jiang
Qingyu Wu
Chong Zhang
Yizhi Cai
Junbiao Dai
Identifying and characterizing SCRaMbLEd synthetic yeast using ReSCuES
description The use of synthetic chromosomes and the recombinase-based SCRaMbLE system could enable rapid strain evolution through massive chromosome rearrangements. Here the authors present ReSCuES, which uses auxotrophic markers to rapidly identify yeast with rearrangements for strain engineering.
format article
author Zhouqing Luo
Lihui Wang
Yun Wang
Weimin Zhang
Yakun Guo
Yue Shen
Linghuo Jiang
Qingyu Wu
Chong Zhang
Yizhi Cai
Junbiao Dai
author_facet Zhouqing Luo
Lihui Wang
Yun Wang
Weimin Zhang
Yakun Guo
Yue Shen
Linghuo Jiang
Qingyu Wu
Chong Zhang
Yizhi Cai
Junbiao Dai
author_sort Zhouqing Luo
title Identifying and characterizing SCRaMbLEd synthetic yeast using ReSCuES
title_short Identifying and characterizing SCRaMbLEd synthetic yeast using ReSCuES
title_full Identifying and characterizing SCRaMbLEd synthetic yeast using ReSCuES
title_fullStr Identifying and characterizing SCRaMbLEd synthetic yeast using ReSCuES
title_full_unstemmed Identifying and characterizing SCRaMbLEd synthetic yeast using ReSCuES
title_sort identifying and characterizing scrambled synthetic yeast using rescues
publisher Nature Portfolio
publishDate 2018
url https://doaj.org/article/a9907f577d24483dabdd7564338c8e60
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