Transcriptional Changes in Pearl Millet Leaves under Heat Stress

High-temperature stress negatively affects the growth and development of plants, and therefore threatens global agricultural safety. Cultivating stress-tolerant plants is the current objective of plant breeding programs. Pearl millet is a multi-purpose plant, commonly used as a forage but also an im...

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Autores principales:	Dejun Huang, Min Sun, Ailing Zhang, Jishan Chen, Jian Zhang, Chuang Lin, Huan Zhang, Xiaowen Lu, Xiaoshan Wang, Haidong Yan, Jianan Tang, Linkai Huang
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	heat stress pearl millet transcriptome sequencing protein folding ROS scavenging enzyme flavonoid synthesis pathway Genetics QH426-470
Acceso en línea:	https://doaj.org/article/a993d7502382448c9f6879f12fb3d630
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spelling	oai:doaj.org-article:a993d7502382448c9f6879f12fb3d6302021-11-25T17:41:12ZTranscriptional Changes in Pearl Millet Leaves under Heat Stress10.3390/genes121117162073-4425https://doaj.org/article/a993d7502382448c9f6879f12fb3d6302021-10-01T00:00:00Zhttps://www.mdpi.com/2073-4425/12/11/1716https://doaj.org/toc/2073-4425High-temperature stress negatively affects the growth and development of plants, and therefore threatens global agricultural safety. Cultivating stress-tolerant plants is the current objective of plant breeding programs. Pearl millet is a multi-purpose plant, commonly used as a forage but also an important food staple. This crop is very heat-resistant and has a higher net assimilation rate than corn under high-temperature stress. However, the response of heat resistant pearl millet has so far not been studied at the transcriptional level. In this study, transcriptome sequencing of pearl millet leaves exposed to different lengths of heat treatment (1 h, 48 h and 96 h) was conducted in order to investigate the molecular mechanisms of the heat stress response and to identify key genes related to heat stress. The results showed that the amount of heat stress-induced DEGs in leaves differs with the length of exposure to high temperatures. The highest value of DEGs (8286) was observed for the group exposed to heat stress for 96 h, while the other two treatments showed lower DEGs values of 4659 DEGs after 1 h exposure and 3981 DEGs after 48 h exposure to heat stress. The DEGs were mainly synthesized in protein folding pathways under high-temperature stress after 1 h exposure. Moreover, a large number of genes encoding ROS scavenging enzymes were activated under heat stress for 1 h and 48 h treatments. The flavonoid synthesis pathway of pearl millet was enriched after heat stress for 96 h. This study analyzed the transcription dynamics under short to long-term heat stress to provide a theoretical basis for the heat resistance response of pearl millet.Dejun HuangMin SunAiling ZhangJishan ChenJian ZhangChuang LinHuan ZhangXiaowen LuXiaoshan WangHaidong YanJianan TangLinkai HuangMDPI AGarticleheat stresspearl millettranscriptome sequencingprotein foldingROS scavenging enzymeflavonoid synthesis pathwayGeneticsQH426-470ENGenes, Vol 12, Iss 1716, p 1716 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	heat stress pearl millet transcriptome sequencing protein folding ROS scavenging enzyme flavonoid synthesis pathway Genetics QH426-470
spellingShingle	heat stress pearl millet transcriptome sequencing protein folding ROS scavenging enzyme flavonoid synthesis pathway Genetics QH426-470 Dejun Huang Min Sun Ailing Zhang Jishan Chen Jian Zhang Chuang Lin Huan Zhang Xiaowen Lu Xiaoshan Wang Haidong Yan Jianan Tang Linkai Huang Transcriptional Changes in Pearl Millet Leaves under Heat Stress
description	High-temperature stress negatively affects the growth and development of plants, and therefore threatens global agricultural safety. Cultivating stress-tolerant plants is the current objective of plant breeding programs. Pearl millet is a multi-purpose plant, commonly used as a forage but also an important food staple. This crop is very heat-resistant and has a higher net assimilation rate than corn under high-temperature stress. However, the response of heat resistant pearl millet has so far not been studied at the transcriptional level. In this study, transcriptome sequencing of pearl millet leaves exposed to different lengths of heat treatment (1 h, 48 h and 96 h) was conducted in order to investigate the molecular mechanisms of the heat stress response and to identify key genes related to heat stress. The results showed that the amount of heat stress-induced DEGs in leaves differs with the length of exposure to high temperatures. The highest value of DEGs (8286) was observed for the group exposed to heat stress for 96 h, while the other two treatments showed lower DEGs values of 4659 DEGs after 1 h exposure and 3981 DEGs after 48 h exposure to heat stress. The DEGs were mainly synthesized in protein folding pathways under high-temperature stress after 1 h exposure. Moreover, a large number of genes encoding ROS scavenging enzymes were activated under heat stress for 1 h and 48 h treatments. The flavonoid synthesis pathway of pearl millet was enriched after heat stress for 96 h. This study analyzed the transcription dynamics under short to long-term heat stress to provide a theoretical basis for the heat resistance response of pearl millet.
format	article
author	Dejun Huang Min Sun Ailing Zhang Jishan Chen Jian Zhang Chuang Lin Huan Zhang Xiaowen Lu Xiaoshan Wang Haidong Yan Jianan Tang Linkai Huang
author_facet	Dejun Huang Min Sun Ailing Zhang Jishan Chen Jian Zhang Chuang Lin Huan Zhang Xiaowen Lu Xiaoshan Wang Haidong Yan Jianan Tang Linkai Huang
author_sort	Dejun Huang
title	Transcriptional Changes in Pearl Millet Leaves under Heat Stress
title_short	Transcriptional Changes in Pearl Millet Leaves under Heat Stress
title_full	Transcriptional Changes in Pearl Millet Leaves under Heat Stress
title_fullStr	Transcriptional Changes in Pearl Millet Leaves under Heat Stress
title_full_unstemmed	Transcriptional Changes in Pearl Millet Leaves under Heat Stress
title_sort	transcriptional changes in pearl millet leaves under heat stress
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/a993d7502382448c9f6879f12fb3d630
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Transcriptional Changes in Pearl Millet Leaves under Heat Stress

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