Metagenomic study to evaluate functional capacity of a cyanobacterial bloom during oxidation

Pre-oxidation can be used against cyanobacteria at the water treatment plant intake to improve cell removal efficiency in down flow processes and reduce cyanotoxins concentrations. In this study, shotgun metagenomic sequencing was used to describe the functional capacity of a cyanobacterial bloom (a...

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Autores principales:	Saber Moradinejad, Hana Trigui, Juan Francisco Guerra Maldonado, B. Jesse Shapiro, Yves Terrat, Sébastien Sauvé, Nathalie Fortin, Arash Zamyadi, Sarah Dorner, Michèle Prévost
Formato:	article
Lenguaje:	EN
Publicado:	Elsevier 2021
Materias:	Cyanobacteria Oxidation Function Biomarker Stress Chemical engineering TP155-156
Acceso en línea:	https://doaj.org/article/a9942352854e4d3ab3503cd8a569b886
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spelling	oai:doaj.org-article:a9942352854e4d3ab3503cd8a569b8862021-11-18T04:52:48ZMetagenomic study to evaluate functional capacity of a cyanobacterial bloom during oxidation2666-821110.1016/j.ceja.2021.100151https://doaj.org/article/a9942352854e4d3ab3503cd8a569b8862021-11-01T00:00:00Zhttp://www.sciencedirect.com/science/article/pii/S2666821121000673https://doaj.org/toc/2666-8211Pre-oxidation can be used against cyanobacteria at the water treatment plant intake to improve cell removal efficiency in down flow processes and reduce cyanotoxins concentrations. In this study, shotgun metagenomic sequencing was used to describe the functional capacity of a cyanobacterial bloom (at Lake Champlain, southern Quebec, Canada) before and after pre-oxidation using Cl2, KMnO4 and H2O2. The bloom samples were associated with two functional profile assemblages: that of August 1st (onset of the bloom) characterized by enrichment of genes related to nutrient uptake and that of August 13th-29th (towards the end of the sampling) associated with competition for resources and repair such as Photosynthesis, Protein metabolism and DNA metabolism. Different functional profile responses to oxidation with Cl2, KMnO4 and H2O2 was also identified as two-time points during the bloom (at the August 1st, and August 29th). On August 1st, chlorinated samples showed a progressive shift in functional profile: starting by acquiring and sequestering nutrient sources (e.g. Iron acquisition, carbohydrates) at low chlorine exposure (CT, concentration X contact time) level, followed by showing a stronger tendency toward dormancy and sporulation genes at high CT. Our results showed that following high CT of H2O2, the relative abundance of the cyanobacterial biomarkers decreased, regardless of the dominant cyanobacterial genus. The toxicity of the bloom before and after oxidation samples was assessed by droplet digital PCR (ddPCR) to measure the mcyD gene. Our results showed significant positive correlation between the mcyD gene copies number and microcystin concentrations in the bloom samples (before the oxidation). However, such correlation was not observed after oxidation. These results suggest that ddPCR can only be used to evaluate bloom toxicity before oxidation.Saber MoradinejadHana TriguiJuan Francisco Guerra MaldonadoB. Jesse ShapiroYves TerratSébastien SauvéNathalie FortinArash ZamyadiSarah DornerMichèle PrévostElsevierarticleCyanobacteriaOxidationFunctionBiomarkerStressChemical engineeringTP155-156ENChemical Engineering Journal Advances, Vol 8, Iss , Pp 100151- (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Cyanobacteria Oxidation Function Biomarker Stress Chemical engineering TP155-156
spellingShingle	Cyanobacteria Oxidation Function Biomarker Stress Chemical engineering TP155-156 Saber Moradinejad Hana Trigui Juan Francisco Guerra Maldonado B. Jesse Shapiro Yves Terrat Sébastien Sauvé Nathalie Fortin Arash Zamyadi Sarah Dorner Michèle Prévost Metagenomic study to evaluate functional capacity of a cyanobacterial bloom during oxidation
description	Pre-oxidation can be used against cyanobacteria at the water treatment plant intake to improve cell removal efficiency in down flow processes and reduce cyanotoxins concentrations. In this study, shotgun metagenomic sequencing was used to describe the functional capacity of a cyanobacterial bloom (at Lake Champlain, southern Quebec, Canada) before and after pre-oxidation using Cl2, KMnO4 and H2O2. The bloom samples were associated with two functional profile assemblages: that of August 1st (onset of the bloom) characterized by enrichment of genes related to nutrient uptake and that of August 13th-29th (towards the end of the sampling) associated with competition for resources and repair such as Photosynthesis, Protein metabolism and DNA metabolism. Different functional profile responses to oxidation with Cl2, KMnO4 and H2O2 was also identified as two-time points during the bloom (at the August 1st, and August 29th). On August 1st, chlorinated samples showed a progressive shift in functional profile: starting by acquiring and sequestering nutrient sources (e.g. Iron acquisition, carbohydrates) at low chlorine exposure (CT, concentration X contact time) level, followed by showing a stronger tendency toward dormancy and sporulation genes at high CT. Our results showed that following high CT of H2O2, the relative abundance of the cyanobacterial biomarkers decreased, regardless of the dominant cyanobacterial genus. The toxicity of the bloom before and after oxidation samples was assessed by droplet digital PCR (ddPCR) to measure the mcyD gene. Our results showed significant positive correlation between the mcyD gene copies number and microcystin concentrations in the bloom samples (before the oxidation). However, such correlation was not observed after oxidation. These results suggest that ddPCR can only be used to evaluate bloom toxicity before oxidation.
format	article
author	Saber Moradinejad Hana Trigui Juan Francisco Guerra Maldonado B. Jesse Shapiro Yves Terrat Sébastien Sauvé Nathalie Fortin Arash Zamyadi Sarah Dorner Michèle Prévost
author_facet	Saber Moradinejad Hana Trigui Juan Francisco Guerra Maldonado B. Jesse Shapiro Yves Terrat Sébastien Sauvé Nathalie Fortin Arash Zamyadi Sarah Dorner Michèle Prévost
author_sort	Saber Moradinejad
title	Metagenomic study to evaluate functional capacity of a cyanobacterial bloom during oxidation
title_short	Metagenomic study to evaluate functional capacity of a cyanobacterial bloom during oxidation
title_full	Metagenomic study to evaluate functional capacity of a cyanobacterial bloom during oxidation
title_fullStr	Metagenomic study to evaluate functional capacity of a cyanobacterial bloom during oxidation
title_full_unstemmed	Metagenomic study to evaluate functional capacity of a cyanobacterial bloom during oxidation
title_sort	metagenomic study to evaluate functional capacity of a cyanobacterial bloom during oxidation
publisher	Elsevier
publishDate	2021
url	https://doaj.org/article/a9942352854e4d3ab3503cd8a569b886
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_version_	1718424998160367616

Metagenomic study to evaluate functional capacity of a cyanobacterial bloom during oxidation

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