Injectable Phosphorescence-based Oxygen Biosensors Identify Post Ischemic Reactive Hyperoxia

Abstract Novel injectable biosensors were used to measure interstitial oxygenation before, during, and after transient ischemia. It is well known that reactive hyperemia occurs following a period of ischemia. However, increased blood flow does not necessarily mean increased oxygen tension in the tis...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Jennifer S. Chien, Mahmoud Mohammed, Hysem Eldik, Mohamed M. Ibrahim, Jeremy Martinez, Scott P. Nichols, Natalie Wisniewski, Bruce Klitzman
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/a9a679ae66c54e738d01d9e3926cb8b6
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:a9a679ae66c54e738d01d9e3926cb8b6
record_format	dspace
spelling	oai:doaj.org-article:a9a679ae66c54e738d01d9e3926cb8b62021-12-02T12:31:54ZInjectable Phosphorescence-based Oxygen Biosensors Identify Post Ischemic Reactive Hyperoxia10.1038/s41598-017-08490-02045-2322https://doaj.org/article/a9a679ae66c54e738d01d9e3926cb8b62017-08-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-08490-0https://doaj.org/toc/2045-2322Abstract Novel injectable biosensors were used to measure interstitial oxygenation before, during, and after transient ischemia. It is well known that reactive hyperemia occurs following a period of ischemia. However, increased blood flow does not necessarily mean increased oxygen tension in the tissue. Therefore, the purpose of this study was to test the hypothesis that tissue reactive hyperoxia occurs following release of hind-limb tourniquet occlusions. Rats were injected with bilateral hind-limb biosensors and were simultaneously subjected to a unilateral femoral vessel ligation. After approximately one and three months, the rats underwent a series of oxygenation challenges, including transient hind-limb tourniquet occlusion. Along with the biosensors, near infrared spectroscopy was used to measure percent oxyhemoglobin in capillaries and laser Doppler flowmetry was used to measure blood flow. Post-occlusion reactive hyperemia was observed. It was accompanied by tissue reactive hyperoxia, affirming that the post-occlusion oxygen supply must have exceeded the expected increased oxygen consumption. The measurement of the physiologic phenomenon of reactive hyperoxia could prove clinically beneficial for both diagnosis and optimizing therapy.Jennifer S. ChienMahmoud MohammedHysem EldikMohamed M. IbrahimJeremy MartinezScott P. NicholsNatalie WisniewskiBruce KlitzmanNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-10 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Jennifer S. Chien Mahmoud Mohammed Hysem Eldik Mohamed M. Ibrahim Jeremy Martinez Scott P. Nichols Natalie Wisniewski Bruce Klitzman Injectable Phosphorescence-based Oxygen Biosensors Identify Post Ischemic Reactive Hyperoxia
description	Abstract Novel injectable biosensors were used to measure interstitial oxygenation before, during, and after transient ischemia. It is well known that reactive hyperemia occurs following a period of ischemia. However, increased blood flow does not necessarily mean increased oxygen tension in the tissue. Therefore, the purpose of this study was to test the hypothesis that tissue reactive hyperoxia occurs following release of hind-limb tourniquet occlusions. Rats were injected with bilateral hind-limb biosensors and were simultaneously subjected to a unilateral femoral vessel ligation. After approximately one and three months, the rats underwent a series of oxygenation challenges, including transient hind-limb tourniquet occlusion. Along with the biosensors, near infrared spectroscopy was used to measure percent oxyhemoglobin in capillaries and laser Doppler flowmetry was used to measure blood flow. Post-occlusion reactive hyperemia was observed. It was accompanied by tissue reactive hyperoxia, affirming that the post-occlusion oxygen supply must have exceeded the expected increased oxygen consumption. The measurement of the physiologic phenomenon of reactive hyperoxia could prove clinically beneficial for both diagnosis and optimizing therapy.
format	article
author	Jennifer S. Chien Mahmoud Mohammed Hysem Eldik Mohamed M. Ibrahim Jeremy Martinez Scott P. Nichols Natalie Wisniewski Bruce Klitzman
author_facet	Jennifer S. Chien Mahmoud Mohammed Hysem Eldik Mohamed M. Ibrahim Jeremy Martinez Scott P. Nichols Natalie Wisniewski Bruce Klitzman
author_sort	Jennifer S. Chien
title	Injectable Phosphorescence-based Oxygen Biosensors Identify Post Ischemic Reactive Hyperoxia
title_short	Injectable Phosphorescence-based Oxygen Biosensors Identify Post Ischemic Reactive Hyperoxia
title_full	Injectable Phosphorescence-based Oxygen Biosensors Identify Post Ischemic Reactive Hyperoxia
title_fullStr	Injectable Phosphorescence-based Oxygen Biosensors Identify Post Ischemic Reactive Hyperoxia
title_full_unstemmed	Injectable Phosphorescence-based Oxygen Biosensors Identify Post Ischemic Reactive Hyperoxia
title_sort	injectable phosphorescence-based oxygen biosensors identify post ischemic reactive hyperoxia
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/a9a679ae66c54e738d01d9e3926cb8b6
work_keys_str_mv	AT jenniferschien injectablephosphorescencebasedoxygenbiosensorsidentifypostischemicreactivehyperoxia AT mahmoudmohammed injectablephosphorescencebasedoxygenbiosensorsidentifypostischemicreactivehyperoxia AT hysemeldik injectablephosphorescencebasedoxygenbiosensorsidentifypostischemicreactivehyperoxia AT mohamedmibrahim injectablephosphorescencebasedoxygenbiosensorsidentifypostischemicreactivehyperoxia AT jeremymartinez injectablephosphorescencebasedoxygenbiosensorsidentifypostischemicreactivehyperoxia AT scottpnichols injectablephosphorescencebasedoxygenbiosensorsidentifypostischemicreactivehyperoxia AT nataliewisniewski injectablephosphorescencebasedoxygenbiosensorsidentifypostischemicreactivehyperoxia AT bruceklitzman injectablephosphorescencebasedoxygenbiosensorsidentifypostischemicreactivehyperoxia
_version_	1718394233960792064

Injectable Phosphorescence-based Oxygen Biosensors Identify Post Ischemic Reactive Hyperoxia

Ejemplares similares