CRISPR-Cpf1 assisted genome editing of Corynebacterium glutamicum
Corynebacterium glutamicum is an important industrial microbe, however it has proven difficult to genetically engineer using Cas9 from Streptococcus pyogenes. Here the authors report effective genome engineering of the bacterium using Cpf1 from Francisella novicida.
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Nature Portfolio
2017
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oai:doaj.org-article:a9a9e34e503a4764a901ee70b6240d592021-12-02T14:42:05ZCRISPR-Cpf1 assisted genome editing of Corynebacterium glutamicum10.1038/ncomms151792041-1723https://doaj.org/article/a9a9e34e503a4764a901ee70b6240d592017-05-01T00:00:00Zhttps://doi.org/10.1038/ncomms15179https://doaj.org/toc/2041-1723Corynebacterium glutamicum is an important industrial microbe, however it has proven difficult to genetically engineer using Cas9 from Streptococcus pyogenes. Here the authors report effective genome engineering of the bacterium using Cpf1 from Francisella novicida.Yu JiangFenghui QianJunjie YangYingmiao LiuFeng DongChongmao XuBingbing SunBiao ChenXiaoshu XuYan LiRenxiao WangSheng YangNature PortfolioarticleScienceQENNature Communications, Vol 8, Iss 1, Pp 1-11 (2017) |
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DOAJ |
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DOAJ |
| language |
EN |
| topic |
Science Q |
| spellingShingle |
Science Q Yu Jiang Fenghui Qian Junjie Yang Yingmiao Liu Feng Dong Chongmao Xu Bingbing Sun Biao Chen Xiaoshu Xu Yan Li Renxiao Wang Sheng Yang CRISPR-Cpf1 assisted genome editing of Corynebacterium glutamicum |
| description |
Corynebacterium glutamicum is an important industrial microbe, however it has proven difficult to genetically engineer using Cas9 from Streptococcus pyogenes. Here the authors report effective genome engineering of the bacterium using Cpf1 from Francisella novicida. |
| format |
article |
| author |
Yu Jiang Fenghui Qian Junjie Yang Yingmiao Liu Feng Dong Chongmao Xu Bingbing Sun Biao Chen Xiaoshu Xu Yan Li Renxiao Wang Sheng Yang |
| author_facet |
Yu Jiang Fenghui Qian Junjie Yang Yingmiao Liu Feng Dong Chongmao Xu Bingbing Sun Biao Chen Xiaoshu Xu Yan Li Renxiao Wang Sheng Yang |
| author_sort |
Yu Jiang |
| title |
CRISPR-Cpf1 assisted genome editing of Corynebacterium glutamicum |
| title_short |
CRISPR-Cpf1 assisted genome editing of Corynebacterium glutamicum |
| title_full |
CRISPR-Cpf1 assisted genome editing of Corynebacterium glutamicum |
| title_fullStr |
CRISPR-Cpf1 assisted genome editing of Corynebacterium glutamicum |
| title_full_unstemmed |
CRISPR-Cpf1 assisted genome editing of Corynebacterium glutamicum |
| title_sort |
crispr-cpf1 assisted genome editing of corynebacterium glutamicum |
| publisher |
Nature Portfolio |
| publishDate |
2017 |
| url |
https://doaj.org/article/a9a9e34e503a4764a901ee70b6240d59 |
| work_keys_str_mv |
AT yujiang crisprcpf1assistedgenomeeditingofcorynebacteriumglutamicum AT fenghuiqian crisprcpf1assistedgenomeeditingofcorynebacteriumglutamicum AT junjieyang crisprcpf1assistedgenomeeditingofcorynebacteriumglutamicum AT yingmiaoliu crisprcpf1assistedgenomeeditingofcorynebacteriumglutamicum AT fengdong crisprcpf1assistedgenomeeditingofcorynebacteriumglutamicum AT chongmaoxu crisprcpf1assistedgenomeeditingofcorynebacteriumglutamicum AT bingbingsun crisprcpf1assistedgenomeeditingofcorynebacteriumglutamicum AT biaochen crisprcpf1assistedgenomeeditingofcorynebacteriumglutamicum AT xiaoshuxu crisprcpf1assistedgenomeeditingofcorynebacteriumglutamicum AT yanli crisprcpf1assistedgenomeeditingofcorynebacteriumglutamicum AT renxiaowang crisprcpf1assistedgenomeeditingofcorynebacteriumglutamicum AT shengyang crisprcpf1assistedgenomeeditingofcorynebacteriumglutamicum |
| _version_ |
1718389828714758144 |