A total closed chest sheep model of cardiogenic shock by percutaneous intracoronary ethanol injection
Abstract To develop a reproducible and stable closed chest model of ischemic cardiogenic shock in sheep, with high survival rate and potential insight into human pathology. We established a protocol for multi-step myocardial alcoholisation of the left anterior descending coronary artery by percutane...
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Nature Portfolio
2020
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oai:doaj.org-article:a9ab62cd62b94e569590e9cc481308fc2021-12-02T16:26:21ZA total closed chest sheep model of cardiogenic shock by percutaneous intracoronary ethanol injection10.1038/s41598-020-68571-52045-2322https://doaj.org/article/a9ab62cd62b94e569590e9cc481308fc2020-07-01T00:00:00Zhttps://doi.org/10.1038/s41598-020-68571-5https://doaj.org/toc/2045-2322Abstract To develop a reproducible and stable closed chest model of ischemic cardiogenic shock in sheep, with high survival rate and potential insight into human pathology. We established a protocol for multi-step myocardial alcoholisation of the left anterior descending coronary artery by percutaneous ethanol injection. A thorough hemodynamic assessment was obtained by invasive and non-invasive monitoring devices. Repeated blood samples were obtained to determine haemoglobin and alcohol concentration, electrolytes, blood gas parameters and cardiac troponin I. After sacrifice, tissue was excised for quantification of infarction and histology. Cardiogenic shock was characterized by a significant decrease in mean arterial pressure (− 33%), cardiac output (− 29%), dP/dt max (− 28%), carotid blood flow (− 22%), left ventricular fractional shortening (− 28%), and left ventricle end-systolic pressure–volume relationship (− 51%). Lactate and cardiac troponin I levels increased from 1.4 ± 0.2 to 4.9 ± 0.7 mmol/L (p = 0.001) and from 0.05 ± 0.02 to 14.74 ± 2.59 µg/L (p = 0.001), respectively. All haemodynamic changes were stable over a three-hour period with a 71% survival rate. The necrotic volume (n = 5) represented 24.0 ± 1.9% of total ventricular mass. No sham exhibited any variation under general anaesthesia. We described and characterized, for the first time, a stable, reproducible sheep model of cardiogenic shock obtained by percutaneous intracoronary ethanol administration.Mario RienzoJulien ImbaultYounes El BoustaniAntoine BeurtonCarolina Carlos SampedranoPhilippe PasdoisMathieu PernotOlivier BernusMichel HaïssaguerreThierry CouffinhalAlexandre OuattaraNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 10, Iss 1, Pp 1-13 (2020) |
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Medicine R Science Q Mario Rienzo Julien Imbault Younes El Boustani Antoine Beurton Carolina Carlos Sampedrano Philippe Pasdois Mathieu Pernot Olivier Bernus Michel Haïssaguerre Thierry Couffinhal Alexandre Ouattara A total closed chest sheep model of cardiogenic shock by percutaneous intracoronary ethanol injection |
| description |
Abstract To develop a reproducible and stable closed chest model of ischemic cardiogenic shock in sheep, with high survival rate and potential insight into human pathology. We established a protocol for multi-step myocardial alcoholisation of the left anterior descending coronary artery by percutaneous ethanol injection. A thorough hemodynamic assessment was obtained by invasive and non-invasive monitoring devices. Repeated blood samples were obtained to determine haemoglobin and alcohol concentration, electrolytes, blood gas parameters and cardiac troponin I. After sacrifice, tissue was excised for quantification of infarction and histology. Cardiogenic shock was characterized by a significant decrease in mean arterial pressure (− 33%), cardiac output (− 29%), dP/dt max (− 28%), carotid blood flow (− 22%), left ventricular fractional shortening (− 28%), and left ventricle end-systolic pressure–volume relationship (− 51%). Lactate and cardiac troponin I levels increased from 1.4 ± 0.2 to 4.9 ± 0.7 mmol/L (p = 0.001) and from 0.05 ± 0.02 to 14.74 ± 2.59 µg/L (p = 0.001), respectively. All haemodynamic changes were stable over a three-hour period with a 71% survival rate. The necrotic volume (n = 5) represented 24.0 ± 1.9% of total ventricular mass. No sham exhibited any variation under general anaesthesia. We described and characterized, for the first time, a stable, reproducible sheep model of cardiogenic shock obtained by percutaneous intracoronary ethanol administration. |
| format |
article |
| author |
Mario Rienzo Julien Imbault Younes El Boustani Antoine Beurton Carolina Carlos Sampedrano Philippe Pasdois Mathieu Pernot Olivier Bernus Michel Haïssaguerre Thierry Couffinhal Alexandre Ouattara |
| author_facet |
Mario Rienzo Julien Imbault Younes El Boustani Antoine Beurton Carolina Carlos Sampedrano Philippe Pasdois Mathieu Pernot Olivier Bernus Michel Haïssaguerre Thierry Couffinhal Alexandre Ouattara |
| author_sort |
Mario Rienzo |
| title |
A total closed chest sheep model of cardiogenic shock by percutaneous intracoronary ethanol injection |
| title_short |
A total closed chest sheep model of cardiogenic shock by percutaneous intracoronary ethanol injection |
| title_full |
A total closed chest sheep model of cardiogenic shock by percutaneous intracoronary ethanol injection |
| title_fullStr |
A total closed chest sheep model of cardiogenic shock by percutaneous intracoronary ethanol injection |
| title_full_unstemmed |
A total closed chest sheep model of cardiogenic shock by percutaneous intracoronary ethanol injection |
| title_sort |
total closed chest sheep model of cardiogenic shock by percutaneous intracoronary ethanol injection |
| publisher |
Nature Portfolio |
| publishDate |
2020 |
| url |
https://doaj.org/article/a9ab62cd62b94e569590e9cc481308fc |
| work_keys_str_mv |
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