A miniature dialysis-culture device allows high-density human-induced pluripotent stem cells expansion from growth factor accumulation
Although human pluripotent stem cell culture is routine, daily media changes, high cost of growth factors required in pluripotent cell media, and reproducibility of differentiation are an obstacle for the mass production of hPSCs. Here, Torizal et al. describe a cost-effective dialysis-culture syste...
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Nature Portfolio
2021
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oai:doaj.org-article:a9aeb1e539894f8bb934595d323399c72021-11-21T12:08:35ZA miniature dialysis-culture device allows high-density human-induced pluripotent stem cells expansion from growth factor accumulation10.1038/s42003-021-02848-x2399-3642https://doaj.org/article/a9aeb1e539894f8bb934595d323399c72021-11-01T00:00:00Zhttps://doi.org/10.1038/s42003-021-02848-xhttps://doaj.org/toc/2399-3642Although human pluripotent stem cell culture is routine, daily media changes, high cost of growth factors required in pluripotent cell media, and reproducibility of differentiation are an obstacle for the mass production of hPSCs. Here, Torizal et al. describe a cost-effective dialysis-culture system for human pluripotent stem cells (hPSCs) at a high density.Fuad Gandhi TorizalQiao You LauMasato IbukiYoshikazu KawaiMasato HorikawaMasataka MinamiTatsuo MichiueIkki HoriguchiMasaki NishikawaYasuyuki SakaiNature PortfolioarticleBiology (General)QH301-705.5ENCommunications Biology, Vol 4, Iss 1, Pp 1-13 (2021) |
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Biology (General) QH301-705.5 |
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Biology (General) QH301-705.5 Fuad Gandhi Torizal Qiao You Lau Masato Ibuki Yoshikazu Kawai Masato Horikawa Masataka Minami Tatsuo Michiue Ikki Horiguchi Masaki Nishikawa Yasuyuki Sakai A miniature dialysis-culture device allows high-density human-induced pluripotent stem cells expansion from growth factor accumulation |
description |
Although human pluripotent stem cell culture is routine, daily media changes, high cost of growth factors required in pluripotent cell media, and reproducibility of differentiation are an obstacle for the mass production of hPSCs. Here, Torizal et al. describe a cost-effective dialysis-culture system for human pluripotent stem cells (hPSCs) at a high density. |
format |
article |
author |
Fuad Gandhi Torizal Qiao You Lau Masato Ibuki Yoshikazu Kawai Masato Horikawa Masataka Minami Tatsuo Michiue Ikki Horiguchi Masaki Nishikawa Yasuyuki Sakai |
author_facet |
Fuad Gandhi Torizal Qiao You Lau Masato Ibuki Yoshikazu Kawai Masato Horikawa Masataka Minami Tatsuo Michiue Ikki Horiguchi Masaki Nishikawa Yasuyuki Sakai |
author_sort |
Fuad Gandhi Torizal |
title |
A miniature dialysis-culture device allows high-density human-induced pluripotent stem cells expansion from growth factor accumulation |
title_short |
A miniature dialysis-culture device allows high-density human-induced pluripotent stem cells expansion from growth factor accumulation |
title_full |
A miniature dialysis-culture device allows high-density human-induced pluripotent stem cells expansion from growth factor accumulation |
title_fullStr |
A miniature dialysis-culture device allows high-density human-induced pluripotent stem cells expansion from growth factor accumulation |
title_full_unstemmed |
A miniature dialysis-culture device allows high-density human-induced pluripotent stem cells expansion from growth factor accumulation |
title_sort |
miniature dialysis-culture device allows high-density human-induced pluripotent stem cells expansion from growth factor accumulation |
publisher |
Nature Portfolio |
publishDate |
2021 |
url |
https://doaj.org/article/a9aeb1e539894f8bb934595d323399c7 |
work_keys_str_mv |
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