A miniature dialysis-culture device allows high-density human-induced pluripotent stem cells expansion from growth factor accumulation

Although human pluripotent stem cell culture is routine, daily media changes, high cost of growth factors required in pluripotent cell media, and reproducibility of differentiation are an obstacle for the mass production of hPSCs. Here, Torizal et al. describe a cost-effective dialysis-culture syste...

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Autores principales: Fuad Gandhi Torizal, Qiao You Lau, Masato Ibuki, Yoshikazu Kawai, Masato Horikawa, Masataka Minami, Tatsuo Michiue, Ikki Horiguchi, Masaki Nishikawa, Yasuyuki Sakai
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Lenguaje:EN
Publicado: Nature Portfolio 2021
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Acceso en línea:https://doaj.org/article/a9aeb1e539894f8bb934595d323399c7
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spelling oai:doaj.org-article:a9aeb1e539894f8bb934595d323399c72021-11-21T12:08:35ZA miniature dialysis-culture device allows high-density human-induced pluripotent stem cells expansion from growth factor accumulation10.1038/s42003-021-02848-x2399-3642https://doaj.org/article/a9aeb1e539894f8bb934595d323399c72021-11-01T00:00:00Zhttps://doi.org/10.1038/s42003-021-02848-xhttps://doaj.org/toc/2399-3642Although human pluripotent stem cell culture is routine, daily media changes, high cost of growth factors required in pluripotent cell media, and reproducibility of differentiation are an obstacle for the mass production of hPSCs. Here, Torizal et al. describe a cost-effective dialysis-culture system for human pluripotent stem cells (hPSCs) at a high density.Fuad Gandhi TorizalQiao You LauMasato IbukiYoshikazu KawaiMasato HorikawaMasataka MinamiTatsuo MichiueIkki HoriguchiMasaki NishikawaYasuyuki SakaiNature PortfolioarticleBiology (General)QH301-705.5ENCommunications Biology, Vol 4, Iss 1, Pp 1-13 (2021)
institution DOAJ
collection DOAJ
language EN
topic Biology (General)
QH301-705.5
spellingShingle Biology (General)
QH301-705.5
Fuad Gandhi Torizal
Qiao You Lau
Masato Ibuki
Yoshikazu Kawai
Masato Horikawa
Masataka Minami
Tatsuo Michiue
Ikki Horiguchi
Masaki Nishikawa
Yasuyuki Sakai
A miniature dialysis-culture device allows high-density human-induced pluripotent stem cells expansion from growth factor accumulation
description Although human pluripotent stem cell culture is routine, daily media changes, high cost of growth factors required in pluripotent cell media, and reproducibility of differentiation are an obstacle for the mass production of hPSCs. Here, Torizal et al. describe a cost-effective dialysis-culture system for human pluripotent stem cells (hPSCs) at a high density.
format article
author Fuad Gandhi Torizal
Qiao You Lau
Masato Ibuki
Yoshikazu Kawai
Masato Horikawa
Masataka Minami
Tatsuo Michiue
Ikki Horiguchi
Masaki Nishikawa
Yasuyuki Sakai
author_facet Fuad Gandhi Torizal
Qiao You Lau
Masato Ibuki
Yoshikazu Kawai
Masato Horikawa
Masataka Minami
Tatsuo Michiue
Ikki Horiguchi
Masaki Nishikawa
Yasuyuki Sakai
author_sort Fuad Gandhi Torizal
title A miniature dialysis-culture device allows high-density human-induced pluripotent stem cells expansion from growth factor accumulation
title_short A miniature dialysis-culture device allows high-density human-induced pluripotent stem cells expansion from growth factor accumulation
title_full A miniature dialysis-culture device allows high-density human-induced pluripotent stem cells expansion from growth factor accumulation
title_fullStr A miniature dialysis-culture device allows high-density human-induced pluripotent stem cells expansion from growth factor accumulation
title_full_unstemmed A miniature dialysis-culture device allows high-density human-induced pluripotent stem cells expansion from growth factor accumulation
title_sort miniature dialysis-culture device allows high-density human-induced pluripotent stem cells expansion from growth factor accumulation
publisher Nature Portfolio
publishDate 2021
url https://doaj.org/article/a9aeb1e539894f8bb934595d323399c7
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