Optical tweezers as a new biomedical tool to measure zeta potential of stored red blood cells.
During storage, red blood cells (RBCs) for transfusion purposes suffer progressive deterioration. Sialylated glycoproteins of the RBC membrane are responsible for a negatively charged surface which creates a repulsive electrical zeta potential. These charges help prevent the interaction between RBCs...
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2012
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oai:doaj.org-article:a9aec6ac834642059cdecc0a469979032021-11-18T07:27:16ZOptical tweezers as a new biomedical tool to measure zeta potential of stored red blood cells.1932-620310.1371/journal.pone.0031778https://doaj.org/article/a9aec6ac834642059cdecc0a469979032012-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/22363729/pdf/?tool=EBIhttps://doaj.org/toc/1932-6203During storage, red blood cells (RBCs) for transfusion purposes suffer progressive deterioration. Sialylated glycoproteins of the RBC membrane are responsible for a negatively charged surface which creates a repulsive electrical zeta potential. These charges help prevent the interaction between RBCs and other cells, and especially among each RBCs. Reports in the literature have stated that RBCs sialylated glycoproteins can be sensitive to enzymes released by leukocyte degranulation. Thus, the aim of this study was, by using an optical tweezers as a biomedical tool, to measure the zeta potential in standard RBCs units and in leukocyte reduced RBC units (collected in CPD-SAGM) during storage. Optical tweezers is a sensitive tool that uses light for measuring cell biophysical properties which are important for clinical and research purposes. This is the first study to analyze RBCs membrane charges during storage. In addition, we herein also measured the elasticity of RBCs also collected in CPD-SAGM. In conclusion, the zeta potential decreased 42% and cells were 134% less deformable at the end of storage. The zeta potential from leukodepleted units had a similar profile when compared to units stored without leukoreduction, indicating that leukocyte lyses were not responsible for the zeta potential decay. Flow cytometry measurements of reactive oxygen species suggested that this decay is due to membrane oxidative damages. These results show that measurements of zeta potentials provide new insights about RBCs storage lesion for transfusion purposes.Diego C N SilvaCauêh N JovinoCarlos A L SilvaHeloise P FernandesMilton M FilhoSheyla C LucenaAna Maria D N CostaCarlos L CesarMaria L Barjas-CastroBeate S SantosAdriana FontesPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 7, Iss 2, p e31778 (2012) |
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Medicine R Science Q Diego C N Silva Cauêh N Jovino Carlos A L Silva Heloise P Fernandes Milton M Filho Sheyla C Lucena Ana Maria D N Costa Carlos L Cesar Maria L Barjas-Castro Beate S Santos Adriana Fontes Optical tweezers as a new biomedical tool to measure zeta potential of stored red blood cells. |
| description |
During storage, red blood cells (RBCs) for transfusion purposes suffer progressive deterioration. Sialylated glycoproteins of the RBC membrane are responsible for a negatively charged surface which creates a repulsive electrical zeta potential. These charges help prevent the interaction between RBCs and other cells, and especially among each RBCs. Reports in the literature have stated that RBCs sialylated glycoproteins can be sensitive to enzymes released by leukocyte degranulation. Thus, the aim of this study was, by using an optical tweezers as a biomedical tool, to measure the zeta potential in standard RBCs units and in leukocyte reduced RBC units (collected in CPD-SAGM) during storage. Optical tweezers is a sensitive tool that uses light for measuring cell biophysical properties which are important for clinical and research purposes. This is the first study to analyze RBCs membrane charges during storage. In addition, we herein also measured the elasticity of RBCs also collected in CPD-SAGM. In conclusion, the zeta potential decreased 42% and cells were 134% less deformable at the end of storage. The zeta potential from leukodepleted units had a similar profile when compared to units stored without leukoreduction, indicating that leukocyte lyses were not responsible for the zeta potential decay. Flow cytometry measurements of reactive oxygen species suggested that this decay is due to membrane oxidative damages. These results show that measurements of zeta potentials provide new insights about RBCs storage lesion for transfusion purposes. |
| format |
article |
| author |
Diego C N Silva Cauêh N Jovino Carlos A L Silva Heloise P Fernandes Milton M Filho Sheyla C Lucena Ana Maria D N Costa Carlos L Cesar Maria L Barjas-Castro Beate S Santos Adriana Fontes |
| author_facet |
Diego C N Silva Cauêh N Jovino Carlos A L Silva Heloise P Fernandes Milton M Filho Sheyla C Lucena Ana Maria D N Costa Carlos L Cesar Maria L Barjas-Castro Beate S Santos Adriana Fontes |
| author_sort |
Diego C N Silva |
| title |
Optical tweezers as a new biomedical tool to measure zeta potential of stored red blood cells. |
| title_short |
Optical tweezers as a new biomedical tool to measure zeta potential of stored red blood cells. |
| title_full |
Optical tweezers as a new biomedical tool to measure zeta potential of stored red blood cells. |
| title_fullStr |
Optical tweezers as a new biomedical tool to measure zeta potential of stored red blood cells. |
| title_full_unstemmed |
Optical tweezers as a new biomedical tool to measure zeta potential of stored red blood cells. |
| title_sort |
optical tweezers as a new biomedical tool to measure zeta potential of stored red blood cells. |
| publisher |
Public Library of Science (PLoS) |
| publishDate |
2012 |
| url |
https://doaj.org/article/a9aec6ac834642059cdecc0a46997903 |
| work_keys_str_mv |
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