Protein identification by 3D OrbiSIMS to facilitate in situ imaging and depth profiling

Label-free protein characterization at surfaces requires digestion or matrix application prior to mass spectrometry. Here, the authors report the assignment of undigested proteins at surfaces by de novo sequencing and apply the methodology to a protein monolayer biochip and for in situ depth profili...

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Autores principales:	Anna M. Kotowska, Gustavo F. Trindade, Paula M. Mendes, Philip M. Williams, Jonathan W. Aylott, Alexander G. Shard, Morgan R. Alexander, David J. Scurr
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2020
Materias:	Science Q
Acceso en línea:	https://doaj.org/article/a9b0c0983019431ea85e4e67217dcf3d
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spelling	oai:doaj.org-article:a9b0c0983019431ea85e4e67217dcf3d2021-12-02T14:41:25ZProtein identification by 3D OrbiSIMS to facilitate in situ imaging and depth profiling10.1038/s41467-020-19445-x2041-1723https://doaj.org/article/a9b0c0983019431ea85e4e67217dcf3d2020-11-01T00:00:00Zhttps://doi.org/10.1038/s41467-020-19445-xhttps://doaj.org/toc/2041-1723Label-free protein characterization at surfaces requires digestion or matrix application prior to mass spectrometry. Here, the authors report the assignment of undigested proteins at surfaces by de novo sequencing and apply the methodology to a protein monolayer biochip and for in situ depth profiling of proteins through human skin.Anna M. KotowskaGustavo F. TrindadePaula M. MendesPhilip M. WilliamsJonathan W. AylottAlexander G. ShardMorgan R. AlexanderDavid J. ScurrNature PortfolioarticleScienceQENNature Communications, Vol 11, Iss 1, Pp 1-8 (2020)
institution	DOAJ
collection	DOAJ
language	EN
topic	Science Q
spellingShingle	Science Q Anna M. Kotowska Gustavo F. Trindade Paula M. Mendes Philip M. Williams Jonathan W. Aylott Alexander G. Shard Morgan R. Alexander David J. Scurr Protein identification by 3D OrbiSIMS to facilitate in situ imaging and depth profiling
description	Label-free protein characterization at surfaces requires digestion or matrix application prior to mass spectrometry. Here, the authors report the assignment of undigested proteins at surfaces by de novo sequencing and apply the methodology to a protein monolayer biochip and for in situ depth profiling of proteins through human skin.
format	article
author	Anna M. Kotowska Gustavo F. Trindade Paula M. Mendes Philip M. Williams Jonathan W. Aylott Alexander G. Shard Morgan R. Alexander David J. Scurr
author_facet	Anna M. Kotowska Gustavo F. Trindade Paula M. Mendes Philip M. Williams Jonathan W. Aylott Alexander G. Shard Morgan R. Alexander David J. Scurr
author_sort	Anna M. Kotowska
title	Protein identification by 3D OrbiSIMS to facilitate in situ imaging and depth profiling
title_short	Protein identification by 3D OrbiSIMS to facilitate in situ imaging and depth profiling
title_full	Protein identification by 3D OrbiSIMS to facilitate in situ imaging and depth profiling
title_fullStr	Protein identification by 3D OrbiSIMS to facilitate in situ imaging and depth profiling
title_full_unstemmed	Protein identification by 3D OrbiSIMS to facilitate in situ imaging and depth profiling
title_sort	protein identification by 3d orbisims to facilitate in situ imaging and depth profiling
publisher	Nature Portfolio
publishDate	2020
url	https://doaj.org/article/a9b0c0983019431ea85e4e67217dcf3d
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Protein identification by 3D OrbiSIMS to facilitate in situ imaging and depth profiling

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