Molecular analysis and epidemiological typing of Vancomycin-resistant Enterococcus outbreak strains

Abstract Outbreaks of multidrug resistant bacteria including vancomycin-resistant enterococci (VRE) in healthcare institutions are increasing in Norway, despite a low level of resistance compared to other European countries. In this study, we describe epidemiological relatedness of vancomycin-resist...

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Autores principales:	Anbjørg Rangberg, Astri Lervik Larsen, Oliver Kacelnik, Hanne Skarpodde Sæther, Marthe Bjørland, Jetmund Ringstad, Christine Monceyron Jonassen
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2019
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/a9c3670e622c47e393961283e29dbab2
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spelling	oai:doaj.org-article:a9c3670e622c47e393961283e29dbab22021-12-02T16:08:17ZMolecular analysis and epidemiological typing of Vancomycin-resistant Enterococcus outbreak strains10.1038/s41598-019-48436-22045-2322https://doaj.org/article/a9c3670e622c47e393961283e29dbab22019-08-01T00:00:00Zhttps://doi.org/10.1038/s41598-019-48436-2https://doaj.org/toc/2045-2322Abstract Outbreaks of multidrug resistant bacteria including vancomycin-resistant enterococci (VRE) in healthcare institutions are increasing in Norway, despite a low level of resistance compared to other European countries. In this study, we describe epidemiological relatedness of vancomycin-resistant Enterococcus faecium isolated during an outbreak at a Norwegian hospital in 2012–2013. During the outbreak, 9454 fecal samples were screened for VRE by culture and/or PCR. Isolates from 86 patients carrying the vanA resistance gene were characterized using pulsed-field gel electrophoresis (PFGE), MALDI-TOF mass spectrometry and single nucleotide polymorphism typing. PFGE revealed two main clusters, the first comprised 56 isolates related to an initial outbreak strain, and the second comprised 21 isolates originating from a later introduced strain, together causing two partly overlapping outbreaks. Nine isolates, including the index case were not related to the two outbreak clusters. In conclusion, the epidemiological analyses show that the outbreak was discovered by coincidence, and that infection control measures were successful. All typing methods identified the two outbreak clusters, and the experiment congruence between the MALDI-TOF and the PFGE clustering was 63.2%, with a strong correlation (r = 72.4%). Despite lower resolution compared to PFGE, MALDI-TOF may provide an efficient mean for real-time monitoring spread of infection.Anbjørg RangbergAstri Lervik LarsenOliver KacelnikHanne Skarpodde SætherMarthe BjørlandJetmund RingstadChristine Monceyron JonassenNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 9, Iss 1, Pp 1-11 (2019)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Anbjørg Rangberg Astri Lervik Larsen Oliver Kacelnik Hanne Skarpodde Sæther Marthe Bjørland Jetmund Ringstad Christine Monceyron Jonassen Molecular analysis and epidemiological typing of Vancomycin-resistant Enterococcus outbreak strains
description	Abstract Outbreaks of multidrug resistant bacteria including vancomycin-resistant enterococci (VRE) in healthcare institutions are increasing in Norway, despite a low level of resistance compared to other European countries. In this study, we describe epidemiological relatedness of vancomycin-resistant Enterococcus faecium isolated during an outbreak at a Norwegian hospital in 2012–2013. During the outbreak, 9454 fecal samples were screened for VRE by culture and/or PCR. Isolates from 86 patients carrying the vanA resistance gene were characterized using pulsed-field gel electrophoresis (PFGE), MALDI-TOF mass spectrometry and single nucleotide polymorphism typing. PFGE revealed two main clusters, the first comprised 56 isolates related to an initial outbreak strain, and the second comprised 21 isolates originating from a later introduced strain, together causing two partly overlapping outbreaks. Nine isolates, including the index case were not related to the two outbreak clusters. In conclusion, the epidemiological analyses show that the outbreak was discovered by coincidence, and that infection control measures were successful. All typing methods identified the two outbreak clusters, and the experiment congruence between the MALDI-TOF and the PFGE clustering was 63.2%, with a strong correlation (r = 72.4%). Despite lower resolution compared to PFGE, MALDI-TOF may provide an efficient mean for real-time monitoring spread of infection.
format	article
author	Anbjørg Rangberg Astri Lervik Larsen Oliver Kacelnik Hanne Skarpodde Sæther Marthe Bjørland Jetmund Ringstad Christine Monceyron Jonassen
author_facet	Anbjørg Rangberg Astri Lervik Larsen Oliver Kacelnik Hanne Skarpodde Sæther Marthe Bjørland Jetmund Ringstad Christine Monceyron Jonassen
author_sort	Anbjørg Rangberg
title	Molecular analysis and epidemiological typing of Vancomycin-resistant Enterococcus outbreak strains
title_short	Molecular analysis and epidemiological typing of Vancomycin-resistant Enterococcus outbreak strains
title_full	Molecular analysis and epidemiological typing of Vancomycin-resistant Enterococcus outbreak strains
title_fullStr	Molecular analysis and epidemiological typing of Vancomycin-resistant Enterococcus outbreak strains
title_full_unstemmed	Molecular analysis and epidemiological typing of Vancomycin-resistant Enterococcus outbreak strains
title_sort	molecular analysis and epidemiological typing of vancomycin-resistant enterococcus outbreak strains
publisher	Nature Portfolio
publishDate	2019
url	https://doaj.org/article/a9c3670e622c47e393961283e29dbab2
work_keys_str_mv	AT anbjørgrangberg molecularanalysisandepidemiologicaltypingofvancomycinresistantenterococcusoutbreakstrains AT astrilerviklarsen molecularanalysisandepidemiologicaltypingofvancomycinresistantenterococcusoutbreakstrains AT oliverkacelnik molecularanalysisandepidemiologicaltypingofvancomycinresistantenterococcusoutbreakstrains AT hanneskarpoddesæther molecularanalysisandepidemiologicaltypingofvancomycinresistantenterococcusoutbreakstrains AT marthebjørland molecularanalysisandepidemiologicaltypingofvancomycinresistantenterococcusoutbreakstrains AT jetmundringstad molecularanalysisandepidemiologicaltypingofvancomycinresistantenterococcusoutbreakstrains AT christinemonceyronjonassen molecularanalysisandepidemiologicaltypingofvancomycinresistantenterococcusoutbreakstrains
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Molecular analysis and epidemiological typing of Vancomycin-resistant Enterococcus outbreak strains

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