Utilizing CRISPR-Cas in Tropical Crop Improvement: A Decision Process for Fitting Genome Engineering to Your Species

Adopting modern gene-editing technologies for trait improvement in agriculture requires important workflow developments, yet these developments are not often discussed. Using tropical crop systems as a case study, we describe a workflow broken down into discrete processes with specific steps and dec...

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Autores principales:	Kathleen A. Joo, Michael G. Muszynski, Michael B. Kantar, Ming-Li Wang, Xiaoling He, Angel R. Del Valle Echevarria
Formato:	article
Lenguaje:	EN
Publicado:	Frontiers Media S.A. 2021
Materias:	non-commodity science democratization orphan crop genome engineering CRISPR-cas tropical crop Genetics QH426-470
Acceso en línea:	https://doaj.org/article/a9c43363d0414af8abc0861b8c96c999
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spelling	oai:doaj.org-article:a9c43363d0414af8abc0861b8c96c9992021-11-12T06:00:33ZUtilizing CRISPR-Cas in Tropical Crop Improvement: A Decision Process for Fitting Genome Engineering to Your Species1664-802110.3389/fgene.2021.786140https://doaj.org/article/a9c43363d0414af8abc0861b8c96c9992021-11-01T00:00:00Zhttps://www.frontiersin.org/articles/10.3389/fgene.2021.786140/fullhttps://doaj.org/toc/1664-8021Adopting modern gene-editing technologies for trait improvement in agriculture requires important workflow developments, yet these developments are not often discussed. Using tropical crop systems as a case study, we describe a workflow broken down into discrete processes with specific steps and decision points that allow for the practical application of the CRISPR-Cas gene editing platform in a crop of interest. While we present the steps of developing genome-edited plants as sequential, in practice parts can be done in parallel, which are discussed in this perspective. The main processes include 1) understanding the genetic basis of the trait along with having the crop’s genome sequence, 2) testing and optimization of the editing reagents, development of efficient 3) tissue culture and 4) transformation methods, and 5) screening methods to identify edited events with commercial potential. Our goal in this perspective is to help any lab that wishes to implement this powerful, easy-to-use tool in their pipeline, thus aiming to democratize the technology.Kathleen A. JooMichael G. MuszynskiMichael B. KantarMing-Li WangXiaoling HeAngel R. Del Valle EchevarriaAngel R. Del Valle EchevarriaFrontiers Media S.A.articlenon-commodityscience democratizationorphan cropgenome engineeringCRISPR-castropical cropGeneticsQH426-470ENFrontiers in Genetics, Vol 12 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	non-commodity science democratization orphan crop genome engineering CRISPR-cas tropical crop Genetics QH426-470
spellingShingle	non-commodity science democratization orphan crop genome engineering CRISPR-cas tropical crop Genetics QH426-470 Kathleen A. Joo Michael G. Muszynski Michael B. Kantar Ming-Li Wang Xiaoling He Angel R. Del Valle Echevarria Angel R. Del Valle Echevarria Utilizing CRISPR-Cas in Tropical Crop Improvement: A Decision Process for Fitting Genome Engineering to Your Species
description	Adopting modern gene-editing technologies for trait improvement in agriculture requires important workflow developments, yet these developments are not often discussed. Using tropical crop systems as a case study, we describe a workflow broken down into discrete processes with specific steps and decision points that allow for the practical application of the CRISPR-Cas gene editing platform in a crop of interest. While we present the steps of developing genome-edited plants as sequential, in practice parts can be done in parallel, which are discussed in this perspective. The main processes include 1) understanding the genetic basis of the trait along with having the crop’s genome sequence, 2) testing and optimization of the editing reagents, development of efficient 3) tissue culture and 4) transformation methods, and 5) screening methods to identify edited events with commercial potential. Our goal in this perspective is to help any lab that wishes to implement this powerful, easy-to-use tool in their pipeline, thus aiming to democratize the technology.
format	article
author	Kathleen A. Joo Michael G. Muszynski Michael B. Kantar Ming-Li Wang Xiaoling He Angel R. Del Valle Echevarria Angel R. Del Valle Echevarria
author_facet	Kathleen A. Joo Michael G. Muszynski Michael B. Kantar Ming-Li Wang Xiaoling He Angel R. Del Valle Echevarria Angel R. Del Valle Echevarria
author_sort	Kathleen A. Joo
title	Utilizing CRISPR-Cas in Tropical Crop Improvement: A Decision Process for Fitting Genome Engineering to Your Species
title_short	Utilizing CRISPR-Cas in Tropical Crop Improvement: A Decision Process for Fitting Genome Engineering to Your Species
title_full	Utilizing CRISPR-Cas in Tropical Crop Improvement: A Decision Process for Fitting Genome Engineering to Your Species
title_fullStr	Utilizing CRISPR-Cas in Tropical Crop Improvement: A Decision Process for Fitting Genome Engineering to Your Species
title_full_unstemmed	Utilizing CRISPR-Cas in Tropical Crop Improvement: A Decision Process for Fitting Genome Engineering to Your Species
title_sort	utilizing crispr-cas in tropical crop improvement: a decision process for fitting genome engineering to your species
publisher	Frontiers Media S.A.
publishDate	2021
url	https://doaj.org/article/a9c43363d0414af8abc0861b8c96c999
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Utilizing CRISPR-Cas in Tropical Crop Improvement: A Decision Process for Fitting Genome Engineering to Your Species

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