Modulating the Biomechanical Properties of Engineered Connective Tissues by Chitosan-Coated Multiwall Carbon Nanotubes

Naim Kittana, 1 Mohyeddin Assali, 2 Wolfram-Hubertus Zimmermann, 3, 4 Norman Liaw, 3, 4 Gabriela Leao Santos, 3, 4 Abdul Rehman, 3, 4 Susanne Lutz 3, 4 1Department of Biomedical Sciences, Faculty of Medicine & Health Sciences, An-Najah National University, Nablus, Palestine; 2Department of...

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Autores principales:	Kittana N, Assali M, Zimmermann WH, Liaw N, Santos GL, Rehman A, Lutz S
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2021
Materias:	engineered connective tissue multiwall carbon nanotubes chitosan mechanical properties collagen-based tissue scaffold Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/a9c7e6ee0cec46b6bd6391ff93494454
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spelling	oai:doaj.org-article:a9c7e6ee0cec46b6bd6391ff934944542021-12-02T13:57:11ZModulating the Biomechanical Properties of Engineered Connective Tissues by Chitosan-Coated Multiwall Carbon Nanotubes1178-2013https://doaj.org/article/a9c7e6ee0cec46b6bd6391ff934944542021-02-01T00:00:00Zhttps://www.dovepress.com/modulating-the-biomechanical-properties-of-engineered-connective-tissu-peer-reviewed-article-IJNhttps://doaj.org/toc/1178-2013Naim Kittana, 1 Mohyeddin Assali, 2 Wolfram-Hubertus Zimmermann, 3, 4 Norman Liaw, 3, 4 Gabriela Leao Santos, 3, 4 Abdul Rehman, 3, 4 Susanne Lutz 3, 4 1Department of Biomedical Sciences, Faculty of Medicine & Health Sciences, An-Najah National University, Nablus, Palestine; 2Department of Pharmacy, Faculty of Medicine & Health Sciences, An-Najah National University, Nablus, Palestine; 3Institute of Pharmacology and Toxicology, University Medical Center Göttingen, Göttingen, Germany; 4DZHK (German Center for Cardiovascular Research) Partner Site Göttingen, Göttingen, GermanyCorrespondence: Naim KittanaDepartment of Biomedical Sciences, Faculty of Medicine and Health Sciences, An Najah National University, Akademia Street, Nablus, PalestineTel +970 (9) 2345113Fax +970 (9) 2345982Email naim.kittana@najah.eduBackground: Under certain conditions, the physiological repair of connective tissues might fail to restore the original structure and function. Optimized engineered connective tissues (ECTs) with biophysical properties adapted to the target tissue could be used as a substitution therapy. This study aimed to investigate the effect of ECT enforcement by a complex of multiwall carbon nanotubes with chitosan (C-MWCNT) to meet in vivo demands.Materials and Methods: ECTs were constructed from human foreskin fibroblasts (HFF-1) in collagen type I and enriched with the three different percentages 0.025, 0.05 and 0.1% of C-MWCNT. Characterization of the physical properties was performed by biomechanical studies using unidirectional strain.Results: Supplementation with 0.025% C-MWCNT moderately increased the tissue stiffness, reflected by Young’s modulus, compared to tissues without C-MWCNT. Supplementation of ECTs with 0.1% C-MWCNT reduced tissue contraction and increased the elasticity and the extensibility, reflected by the yield point and ultimate strain, respectively. Consequently, the ECTs with 0.1% C-MWCNT showed a higher resilience and toughness as control tissues. Fluorescence tissue imaging demonstrated the longitudinal alignment of all cells independent of the condition.Conclusion: Supplementation with C-MWCNT can enhance the biophysical properties of ECTs, which could be advantageous for applications in connective tissue repair.Keywords: engineered connective tissue, multiwall carbon nanotubes, chitosan, mechanical properties, collagen-based tissue scaffoldKittana NAssali MZimmermann WHLiaw NSantos GLRehman ALutz SDove Medical Pressarticleengineered connective tissuemultiwall carbon nanotubeschitosanmechanical propertiescollagen-based tissue scaffoldMedicine (General)R5-920ENInternational Journal of Nanomedicine, Vol Volume 16, Pp 989-1000 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	engineered connective tissue multiwall carbon nanotubes chitosan mechanical properties collagen-based tissue scaffold Medicine (General) R5-920
spellingShingle	engineered connective tissue multiwall carbon nanotubes chitosan mechanical properties collagen-based tissue scaffold Medicine (General) R5-920 Kittana N Assali M Zimmermann WH Liaw N Santos GL Rehman A Lutz S Modulating the Biomechanical Properties of Engineered Connective Tissues by Chitosan-Coated Multiwall Carbon Nanotubes
description	Naim Kittana, 1 Mohyeddin Assali, 2 Wolfram-Hubertus Zimmermann, 3, 4 Norman Liaw, 3, 4 Gabriela Leao Santos, 3, 4 Abdul Rehman, 3, 4 Susanne Lutz 3, 4 1Department of Biomedical Sciences, Faculty of Medicine & Health Sciences, An-Najah National University, Nablus, Palestine; 2Department of Pharmacy, Faculty of Medicine & Health Sciences, An-Najah National University, Nablus, Palestine; 3Institute of Pharmacology and Toxicology, University Medical Center Göttingen, Göttingen, Germany; 4DZHK (German Center for Cardiovascular Research) Partner Site Göttingen, Göttingen, GermanyCorrespondence: Naim KittanaDepartment of Biomedical Sciences, Faculty of Medicine and Health Sciences, An Najah National University, Akademia Street, Nablus, PalestineTel +970 (9) 2345113Fax +970 (9) 2345982Email naim.kittana@najah.eduBackground: Under certain conditions, the physiological repair of connective tissues might fail to restore the original structure and function. Optimized engineered connective tissues (ECTs) with biophysical properties adapted to the target tissue could be used as a substitution therapy. This study aimed to investigate the effect of ECT enforcement by a complex of multiwall carbon nanotubes with chitosan (C-MWCNT) to meet in vivo demands.Materials and Methods: ECTs were constructed from human foreskin fibroblasts (HFF-1) in collagen type I and enriched with the three different percentages 0.025, 0.05 and 0.1% of C-MWCNT. Characterization of the physical properties was performed by biomechanical studies using unidirectional strain.Results: Supplementation with 0.025% C-MWCNT moderately increased the tissue stiffness, reflected by Young’s modulus, compared to tissues without C-MWCNT. Supplementation of ECTs with 0.1% C-MWCNT reduced tissue contraction and increased the elasticity and the extensibility, reflected by the yield point and ultimate strain, respectively. Consequently, the ECTs with 0.1% C-MWCNT showed a higher resilience and toughness as control tissues. Fluorescence tissue imaging demonstrated the longitudinal alignment of all cells independent of the condition.Conclusion: Supplementation with C-MWCNT can enhance the biophysical properties of ECTs, which could be advantageous for applications in connective tissue repair.Keywords: engineered connective tissue, multiwall carbon nanotubes, chitosan, mechanical properties, collagen-based tissue scaffold
format	article
author	Kittana N Assali M Zimmermann WH Liaw N Santos GL Rehman A Lutz S
author_facet	Kittana N Assali M Zimmermann WH Liaw N Santos GL Rehman A Lutz S
author_sort	Kittana N
title	Modulating the Biomechanical Properties of Engineered Connective Tissues by Chitosan-Coated Multiwall Carbon Nanotubes
title_short	Modulating the Biomechanical Properties of Engineered Connective Tissues by Chitosan-Coated Multiwall Carbon Nanotubes
title_full	Modulating the Biomechanical Properties of Engineered Connective Tissues by Chitosan-Coated Multiwall Carbon Nanotubes
title_fullStr	Modulating the Biomechanical Properties of Engineered Connective Tissues by Chitosan-Coated Multiwall Carbon Nanotubes
title_full_unstemmed	Modulating the Biomechanical Properties of Engineered Connective Tissues by Chitosan-Coated Multiwall Carbon Nanotubes
title_sort	modulating the biomechanical properties of engineered connective tissues by chitosan-coated multiwall carbon nanotubes
publisher	Dove Medical Press
publishDate	2021
url	https://doaj.org/article/a9c7e6ee0cec46b6bd6391ff93494454
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Modulating the Biomechanical Properties of Engineered Connective Tissues by Chitosan-Coated Multiwall Carbon Nanotubes

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