Microencapsulated Isoniazid-Loaded Metal–Organic Frameworks for Pulmonary Administration of Antituberculosis Drugs

Tuberculosis (TB) is an infectious disease that causes a great number of deaths in the world (1.5 million people per year). This disease is currently treated by administering high doses of various oral anti-TB drugs for prolonged periods (up to 2 years). While this regimen is normally effective when...

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Autores principales:	Cristina Fernández-Paz, Estefanía Fernández-Paz, Pablo Salcedo-Abraira, Sara Rojas, Sheila Barrios-Esteban, Noemi Csaba, Patricia Horcajada, Carmen Remuñán-López
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	A549 cells isoniazid mannitol metal–organic frameworks microencapsulation pulmonary administration Organic chemistry QD241-441
Acceso en línea:	https://doaj.org/article/a9ca41a8dd55412b966565bcca81cf91
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spelling	oai:doaj.org-article:a9ca41a8dd55412b966565bcca81cf912021-11-11T18:25:41ZMicroencapsulated Isoniazid-Loaded Metal–Organic Frameworks for Pulmonary Administration of Antituberculosis Drugs10.3390/molecules262164081420-3049https://doaj.org/article/a9ca41a8dd55412b966565bcca81cf912021-10-01T00:00:00Zhttps://www.mdpi.com/1420-3049/26/21/6408https://doaj.org/toc/1420-3049Tuberculosis (TB) is an infectious disease that causes a great number of deaths in the world (1.5 million people per year). This disease is currently treated by administering high doses of various oral anti-TB drugs for prolonged periods (up to 2 years). While this regimen is normally effective when taken as prescribed, many people with TB experience difficulties in complying with their medication schedule. Furthermore, the oral administration of standard anti-TB drugs causes severe side effects and widespread resistances. Recently, we proposed an original platform for pulmonary TB treatment consisting of mannitol microspheres (Ma MS) containing iron (III) trimesate metal–organic framework (MOF) MIL-100 nanoparticles (NPs). In the present work, we loaded this system with the first-line anti-TB drug isoniazid (INH) and evaluated both the viability and safety of the drug vehicle components, as well as the cell internalization of the formulation in alveolar A549 cells. Results show that INH-loaded MOF (INH@MIL-100) NPs were efficiently microencapsulated in Ma MS, which displayed suitable aerodynamic characteristics for pulmonary administration and non-toxicity. MIL-100 and INH@MIL-100 NPs were efficiently internalized by A549 cells, mainly localized in the cytoplasm. In conclusion, the proposed micro-nanosystem is a good candidate for the pulmonary administration of anti-TB drugs.Cristina Fernández-PazEstefanía Fernández-PazPablo Salcedo-AbrairaSara RojasSheila Barrios-EstebanNoemi CsabaPatricia HorcajadaCarmen Remuñán-LópezMDPI AGarticleA549 cellsisoniazidmannitolmetal–organic frameworksmicroencapsulationpulmonary administrationOrganic chemistryQD241-441ENMolecules, Vol 26, Iss 6408, p 6408 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	A549 cells isoniazid mannitol metal–organic frameworks microencapsulation pulmonary administration Organic chemistry QD241-441
spellingShingle	A549 cells isoniazid mannitol metal–organic frameworks microencapsulation pulmonary administration Organic chemistry QD241-441 Cristina Fernández-Paz Estefanía Fernández-Paz Pablo Salcedo-Abraira Sara Rojas Sheila Barrios-Esteban Noemi Csaba Patricia Horcajada Carmen Remuñán-López Microencapsulated Isoniazid-Loaded Metal–Organic Frameworks for Pulmonary Administration of Antituberculosis Drugs
description	Tuberculosis (TB) is an infectious disease that causes a great number of deaths in the world (1.5 million people per year). This disease is currently treated by administering high doses of various oral anti-TB drugs for prolonged periods (up to 2 years). While this regimen is normally effective when taken as prescribed, many people with TB experience difficulties in complying with their medication schedule. Furthermore, the oral administration of standard anti-TB drugs causes severe side effects and widespread resistances. Recently, we proposed an original platform for pulmonary TB treatment consisting of mannitol microspheres (Ma MS) containing iron (III) trimesate metal–organic framework (MOF) MIL-100 nanoparticles (NPs). In the present work, we loaded this system with the first-line anti-TB drug isoniazid (INH) and evaluated both the viability and safety of the drug vehicle components, as well as the cell internalization of the formulation in alveolar A549 cells. Results show that INH-loaded MOF (INH@MIL-100) NPs were efficiently microencapsulated in Ma MS, which displayed suitable aerodynamic characteristics for pulmonary administration and non-toxicity. MIL-100 and INH@MIL-100 NPs were efficiently internalized by A549 cells, mainly localized in the cytoplasm. In conclusion, the proposed micro-nanosystem is a good candidate for the pulmonary administration of anti-TB drugs.
format	article
author	Cristina Fernández-Paz Estefanía Fernández-Paz Pablo Salcedo-Abraira Sara Rojas Sheila Barrios-Esteban Noemi Csaba Patricia Horcajada Carmen Remuñán-López
author_facet	Cristina Fernández-Paz Estefanía Fernández-Paz Pablo Salcedo-Abraira Sara Rojas Sheila Barrios-Esteban Noemi Csaba Patricia Horcajada Carmen Remuñán-López
author_sort	Cristina Fernández-Paz
title	Microencapsulated Isoniazid-Loaded Metal–Organic Frameworks for Pulmonary Administration of Antituberculosis Drugs
title_short	Microencapsulated Isoniazid-Loaded Metal–Organic Frameworks for Pulmonary Administration of Antituberculosis Drugs
title_full	Microencapsulated Isoniazid-Loaded Metal–Organic Frameworks for Pulmonary Administration of Antituberculosis Drugs
title_fullStr	Microencapsulated Isoniazid-Loaded Metal–Organic Frameworks for Pulmonary Administration of Antituberculosis Drugs
title_full_unstemmed	Microencapsulated Isoniazid-Loaded Metal–Organic Frameworks for Pulmonary Administration of Antituberculosis Drugs
title_sort	microencapsulated isoniazid-loaded metal–organic frameworks for pulmonary administration of antituberculosis drugs
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/a9ca41a8dd55412b966565bcca81cf91
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Microencapsulated Isoniazid-Loaded Metal–Organic Frameworks for Pulmonary Administration of Antituberculosis Drugs

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