Molecular analysis of Taro and Bali cattle using cytochrome oxidase subunit I (COI) in Indonesia

Abstract. Susari NNW, Suastika P, Agustina KK. 2021. Molecular analysis of Taro and Bali cattle using cytochrome oxidase subunit I (COI) in Indonesia. Biodiversitas 22: 165-172. Cytochrome oxidase subunit I (COI) is one of the molecular markers often used as a differentiator with many advantages in...

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Autores principales:	Ni Nyoman Werdi Susari, I Putu Suastika, Kadek Karang Agustina
Formato:	article
Lenguaje:	EN
Publicado:	MBI & UNS Solo 2020
Materias:	bali cattle, cytochrome oxidase subunit i, mega 6, pcr, phylogenetic, taro cattle Biology (General) QH301-705.5
Acceso en línea:	https://doaj.org/article/a9d0b1d3422645a1983647a550078e40
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spelling	oai:doaj.org-article:a9d0b1d3422645a1983647a550078e402021-11-22T00:53:57ZMolecular analysis of Taro and Bali cattle using cytochrome oxidase subunit I (COI) in Indonesia1412-033X2085-472210.13057/biodiv/d220122https://doaj.org/article/a9d0b1d3422645a1983647a550078e402020-12-01T00:00:00Zhttps://smujo.id/biodiv/article/view/6948https://doaj.org/toc/1412-033Xhttps://doaj.org/toc/2085-4722Abstract. Susari NNW, Suastika P, Agustina KK. 2021. Molecular analysis of Taro and Bali cattle using cytochrome oxidase subunit I (COI) in Indonesia. Biodiversitas 22: 165-172. Cytochrome oxidase subunit I (COI) is one of the molecular markers often used as a differentiator with many advantages in phylogeny analysis, and it thus rarely undergoes substitution. This research was conducted to characterize the genetics of Taro and Bali (Bos javanicus) cattle. Blood from the animals was collected from the jugularis vein and amplified by PCR. The target area was COI with a primer that was successfully amplified, namely the forward BICOIF (5'-TTC-TCAACCAACCATAAAGATATTGG-3') and the reverse BICOIR fragment (5'-TAG-ACTTCGGGGTGTCCAAAGAATCA-3'). The PCR products' sequencing was carried out by phylogeny analysis using MEGA 6 software. The amplicon value that succeeded in electrophoresis was 710bp, while six polymorphic sites were obtained at base positions of 1, 300, 379, 675, 676, and 679. The haplotypes (Hap) obtained were 4, with a genetic distance that ranged from 0.000-0.001. The nitrogenous bases of the amino acid composition from the samples showed no significant difference. The phylogenetic tree (Tamura-Nei) classified the cattle into two clades with a genetic distance of 0.0005.Ni Nyoman Werdi SusariI Putu SuastikaKadek Karang AgustinaMBI & UNS Soloarticlebali cattle, cytochrome oxidase subunit i, mega 6, pcr, phylogenetic, taro cattleBiology (General)QH301-705.5ENBiodiversitas, Vol 22, Iss 1 (2020)
institution	DOAJ
collection	DOAJ
language	EN
topic	bali cattle, cytochrome oxidase subunit i, mega 6, pcr, phylogenetic, taro cattle Biology (General) QH301-705.5
spellingShingle	bali cattle, cytochrome oxidase subunit i, mega 6, pcr, phylogenetic, taro cattle Biology (General) QH301-705.5 Ni Nyoman Werdi Susari I Putu Suastika Kadek Karang Agustina Molecular analysis of Taro and Bali cattle using cytochrome oxidase subunit I (COI) in Indonesia
description	Abstract. Susari NNW, Suastika P, Agustina KK. 2021. Molecular analysis of Taro and Bali cattle using cytochrome oxidase subunit I (COI) in Indonesia. Biodiversitas 22: 165-172. Cytochrome oxidase subunit I (COI) is one of the molecular markers often used as a differentiator with many advantages in phylogeny analysis, and it thus rarely undergoes substitution. This research was conducted to characterize the genetics of Taro and Bali (Bos javanicus) cattle. Blood from the animals was collected from the jugularis vein and amplified by PCR. The target area was COI with a primer that was successfully amplified, namely the forward BICOIF (5'-TTC-TCAACCAACCATAAAGATATTGG-3') and the reverse BICOIR fragment (5'-TAG-ACTTCGGGGTGTCCAAAGAATCA-3'). The PCR products' sequencing was carried out by phylogeny analysis using MEGA 6 software. The amplicon value that succeeded in electrophoresis was 710bp, while six polymorphic sites were obtained at base positions of 1, 300, 379, 675, 676, and 679. The haplotypes (Hap) obtained were 4, with a genetic distance that ranged from 0.000-0.001. The nitrogenous bases of the amino acid composition from the samples showed no significant difference. The phylogenetic tree (Tamura-Nei) classified the cattle into two clades with a genetic distance of 0.0005.
format	article
author	Ni Nyoman Werdi Susari I Putu Suastika Kadek Karang Agustina
author_facet	Ni Nyoman Werdi Susari I Putu Suastika Kadek Karang Agustina
author_sort	Ni Nyoman Werdi Susari
title	Molecular analysis of Taro and Bali cattle using cytochrome oxidase subunit I (COI) in Indonesia
title_short	Molecular analysis of Taro and Bali cattle using cytochrome oxidase subunit I (COI) in Indonesia
title_full	Molecular analysis of Taro and Bali cattle using cytochrome oxidase subunit I (COI) in Indonesia
title_fullStr	Molecular analysis of Taro and Bali cattle using cytochrome oxidase subunit I (COI) in Indonesia
title_full_unstemmed	Molecular analysis of Taro and Bali cattle using cytochrome oxidase subunit I (COI) in Indonesia
title_sort	molecular analysis of taro and bali cattle using cytochrome oxidase subunit i (coi) in indonesia
publisher	MBI & UNS Solo
publishDate	2020
url	https://doaj.org/article/a9d0b1d3422645a1983647a550078e40
work_keys_str_mv	AT ninyomanwerdisusari molecularanalysisoftaroandbalicattleusingcytochromeoxidasesubuniticoiinindonesia AT iputusuastika molecularanalysisoftaroandbalicattleusingcytochromeoxidasesubuniticoiinindonesia AT kadekkarangagustina molecularanalysisoftaroandbalicattleusingcytochromeoxidasesubuniticoiinindonesia
_version_	1718418478938980352

Molecular analysis of Taro and Bali cattle using cytochrome oxidase subunit I (COI) in Indonesia

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