Intranasal vaccination with a Newcastle disease virus-vectored vaccine protects hamsters from SARS-CoV-2 infection and disease

Summary: The pandemic severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is the cause of coronavirus disease 2019 (COVID-19). Worldwide efforts are being made to develop vaccines to mitigate this pandemic. We engineered two recombinant Newcastle disease virus (NDV) vectors expressing eithe...

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Autores principales:	Bryce M. Warner, Lisa A. Santry, Alexander Leacy, Mable Chan, Phuc H. Pham, Robert Vendramelli, Yanlong Pei, Nikesh Tailor, Emelissa Valcourt, Anders Leung, Shihua He, Bryan D. Griffin, Jonathan Audet, Marnie Willman, Kevin Tierney, Alixandra Albietz, Kathy L. Frost, Jacob G.E. Yates, Robert C. Mould, Lily Chan, Yeganeh Mehrani, Jason P. Knapp, Jessica A. Minott, Logan Banadyga, David Safronetz, Heidi Wood, Stephanie Booth, Pierre P. Major, Byram W. Bridle, Leonardo Susta, Darwyn Kobasa, Sarah K. Wootton
Formato:	article
Lenguaje:	EN
Publicado:	Elsevier 2021
Materias:	Immune respons Virology Science Q
Acceso en línea:	https://doaj.org/article/a9d6cd355eb7470ba0489696155667f5
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spelling	oai:doaj.org-article:a9d6cd355eb7470ba0489696155667f52021-11-20T05:08:24ZIntranasal vaccination with a Newcastle disease virus-vectored vaccine protects hamsters from SARS-CoV-2 infection and disease2589-004210.1016/j.isci.2021.103219https://doaj.org/article/a9d6cd355eb7470ba0489696155667f52021-11-01T00:00:00Zhttp://www.sciencedirect.com/science/article/pii/S2589004221011871https://doaj.org/toc/2589-0042Summary: The pandemic severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is the cause of coronavirus disease 2019 (COVID-19). Worldwide efforts are being made to develop vaccines to mitigate this pandemic. We engineered two recombinant Newcastle disease virus (NDV) vectors expressing either the full-length SARS-CoV-2 spike protein (NDV-FLS) or a version with a 19 amino acid deletion at the carboxy terminus (NDV-Δ19S). Hamsters receiving two doses (prime-boost) of NDV-FLS developed a robust SARS-CoV-2-neutralizing antibody response, with elimination of infectious virus in the lungs and minimal lung pathology at five days post-challenge. Single-dose vaccination with NDV-FLS significantly reduced SARS-CoV-2 replication in the lungs but only mildly decreased lung inflammation. NDV-Δ19S-treated hamsters had a moderate decrease in SARS-CoV-2 titers in lungs and presented with severe microscopic lesions, suggesting that truncation of the spike protein was a less effective strategy. In summary, NDV-vectored vaccines represent a viable option for protection against COVID-19.Bryce M. WarnerLisa A. SantryAlexander LeacyMable ChanPhuc H. PhamRobert VendramelliYanlong PeiNikesh TailorEmelissa ValcourtAnders LeungShihua HeBryan D. GriffinJonathan AudetMarnie WillmanKevin TierneyAlixandra AlbietzKathy L. FrostJacob G.E. YatesRobert C. MouldLily ChanYeganeh MehraniJason P. KnappJessica A. MinottLogan BanadygaDavid SafronetzHeidi WoodStephanie BoothPierre P. MajorByram W. BridleLeonardo SustaDarwyn KobasaSarah K. WoottonElsevierarticleImmune responsVirologyScienceQENiScience, Vol 24, Iss 11, Pp 103219- (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Immune respons Virology Science Q
spellingShingle	Immune respons Virology Science Q Bryce M. Warner Lisa A. Santry Alexander Leacy Mable Chan Phuc H. Pham Robert Vendramelli Yanlong Pei Nikesh Tailor Emelissa Valcourt Anders Leung Shihua He Bryan D. Griffin Jonathan Audet Marnie Willman Kevin Tierney Alixandra Albietz Kathy L. Frost Jacob G.E. Yates Robert C. Mould Lily Chan Yeganeh Mehrani Jason P. Knapp Jessica A. Minott Logan Banadyga David Safronetz Heidi Wood Stephanie Booth Pierre P. Major Byram W. Bridle Leonardo Susta Darwyn Kobasa Sarah K. Wootton Intranasal vaccination with a Newcastle disease virus-vectored vaccine protects hamsters from SARS-CoV-2 infection and disease
description	Summary: The pandemic severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is the cause of coronavirus disease 2019 (COVID-19). Worldwide efforts are being made to develop vaccines to mitigate this pandemic. We engineered two recombinant Newcastle disease virus (NDV) vectors expressing either the full-length SARS-CoV-2 spike protein (NDV-FLS) or a version with a 19 amino acid deletion at the carboxy terminus (NDV-Δ19S). Hamsters receiving two doses (prime-boost) of NDV-FLS developed a robust SARS-CoV-2-neutralizing antibody response, with elimination of infectious virus in the lungs and minimal lung pathology at five days post-challenge. Single-dose vaccination with NDV-FLS significantly reduced SARS-CoV-2 replication in the lungs but only mildly decreased lung inflammation. NDV-Δ19S-treated hamsters had a moderate decrease in SARS-CoV-2 titers in lungs and presented with severe microscopic lesions, suggesting that truncation of the spike protein was a less effective strategy. In summary, NDV-vectored vaccines represent a viable option for protection against COVID-19.
format	article
author	Bryce M. Warner Lisa A. Santry Alexander Leacy Mable Chan Phuc H. Pham Robert Vendramelli Yanlong Pei Nikesh Tailor Emelissa Valcourt Anders Leung Shihua He Bryan D. Griffin Jonathan Audet Marnie Willman Kevin Tierney Alixandra Albietz Kathy L. Frost Jacob G.E. Yates Robert C. Mould Lily Chan Yeganeh Mehrani Jason P. Knapp Jessica A. Minott Logan Banadyga David Safronetz Heidi Wood Stephanie Booth Pierre P. Major Byram W. Bridle Leonardo Susta Darwyn Kobasa Sarah K. Wootton
author_facet	Bryce M. Warner Lisa A. Santry Alexander Leacy Mable Chan Phuc H. Pham Robert Vendramelli Yanlong Pei Nikesh Tailor Emelissa Valcourt Anders Leung Shihua He Bryan D. Griffin Jonathan Audet Marnie Willman Kevin Tierney Alixandra Albietz Kathy L. Frost Jacob G.E. Yates Robert C. Mould Lily Chan Yeganeh Mehrani Jason P. Knapp Jessica A. Minott Logan Banadyga David Safronetz Heidi Wood Stephanie Booth Pierre P. Major Byram W. Bridle Leonardo Susta Darwyn Kobasa Sarah K. Wootton
author_sort	Bryce M. Warner
title	Intranasal vaccination with a Newcastle disease virus-vectored vaccine protects hamsters from SARS-CoV-2 infection and disease
title_short	Intranasal vaccination with a Newcastle disease virus-vectored vaccine protects hamsters from SARS-CoV-2 infection and disease
title_full	Intranasal vaccination with a Newcastle disease virus-vectored vaccine protects hamsters from SARS-CoV-2 infection and disease
title_fullStr	Intranasal vaccination with a Newcastle disease virus-vectored vaccine protects hamsters from SARS-CoV-2 infection and disease
title_full_unstemmed	Intranasal vaccination with a Newcastle disease virus-vectored vaccine protects hamsters from SARS-CoV-2 infection and disease
title_sort	intranasal vaccination with a newcastle disease virus-vectored vaccine protects hamsters from sars-cov-2 infection and disease
publisher	Elsevier
publishDate	2021
url	https://doaj.org/article/a9d6cd355eb7470ba0489696155667f5
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Intranasal vaccination with a Newcastle disease virus-vectored vaccine protects hamsters from SARS-CoV-2 infection and disease

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