Inactivation of Pol θ and C-NHEJ eliminates off-target integration of exogenous DNA

Random off-target integration events can impair precise gene targeting and poses a safety risk for gene therapy. Here the authors show that repression of polymerase θ and classical non-homologous recombination eliminates random integration.

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Autores principales:	Alex N. Zelensky, Joost Schimmel, Hanneke Kool, Roland Kanaar, Marcel Tijsterman
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Science Q
Acceso en línea:	https://doaj.org/article/a9da6d7f29cb4983892c270e8629d44c
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spelling	oai:doaj.org-article:a9da6d7f29cb4983892c270e8629d44c2021-12-02T13:24:33ZInactivation of Pol θ and C-NHEJ eliminates off-target integration of exogenous DNA10.1038/s41467-017-00124-32041-1723https://doaj.org/article/a9da6d7f29cb4983892c270e8629d44c2017-07-01T00:00:00Zhttps://doi.org/10.1038/s41467-017-00124-3https://doaj.org/toc/2041-1723Random off-target integration events can impair precise gene targeting and poses a safety risk for gene therapy. Here the authors show that repression of polymerase θ and classical non-homologous recombination eliminates random integration.Alex N. ZelenskyJoost SchimmelHanneke KoolRoland KanaarMarcel TijstermanNature PortfolioarticleScienceQENNature Communications, Vol 8, Iss 1, Pp 1-7 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Science Q
spellingShingle	Science Q Alex N. Zelensky Joost Schimmel Hanneke Kool Roland Kanaar Marcel Tijsterman Inactivation of Pol θ and C-NHEJ eliminates off-target integration of exogenous DNA
description	Random off-target integration events can impair precise gene targeting and poses a safety risk for gene therapy. Here the authors show that repression of polymerase θ and classical non-homologous recombination eliminates random integration.
format	article
author	Alex N. Zelensky Joost Schimmel Hanneke Kool Roland Kanaar Marcel Tijsterman
author_facet	Alex N. Zelensky Joost Schimmel Hanneke Kool Roland Kanaar Marcel Tijsterman
author_sort	Alex N. Zelensky
title	Inactivation of Pol θ and C-NHEJ eliminates off-target integration of exogenous DNA
title_short	Inactivation of Pol θ and C-NHEJ eliminates off-target integration of exogenous DNA
title_full	Inactivation of Pol θ and C-NHEJ eliminates off-target integration of exogenous DNA
title_fullStr	Inactivation of Pol θ and C-NHEJ eliminates off-target integration of exogenous DNA
title_full_unstemmed	Inactivation of Pol θ and C-NHEJ eliminates off-target integration of exogenous DNA
title_sort	inactivation of pol θ and c-nhej eliminates off-target integration of exogenous dna
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/a9da6d7f29cb4983892c270e8629d44c
work_keys_str_mv	AT alexnzelensky inactivationofpolthandcnhejeliminatesofftargetintegrationofexogenousdna AT joostschimmel inactivationofpolthandcnhejeliminatesofftargetintegrationofexogenousdna AT hannekekool inactivationofpolthandcnhejeliminatesofftargetintegrationofexogenousdna AT rolandkanaar inactivationofpolthandcnhejeliminatesofftargetintegrationofexogenousdna AT marceltijsterman inactivationofpolthandcnhejeliminatesofftargetintegrationofexogenousdna
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Inactivation of Pol θ and C-NHEJ eliminates off-target integration of exogenous DNA

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