Development and Evaluation of Alternative Methods to Identify the Three Most Common Serotypes of <i>Salmonella enterica</i> Causing Clinical Infections in Kazakhstan
In this study, we aimed to compare the performance of conventional PCR and real-time PCR assays as screening methods for identification of three frequent, clinically significant Salmonella serovars in Kazakhstan. We determined the diagnostic efficacy of three molecular methods for detection of <i...
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| Autores principales: | , , , , , , |
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| Formato: | article |
| Lenguaje: | EN |
| Publicado: |
MDPI AG
2021
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| Materias: | |
| Acceso en línea: | https://doaj.org/article/a9dd2c478df3461fad412cecc80ff95e |
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| Sumario: | In this study, we aimed to compare the performance of conventional PCR and real-time PCR assays as screening methods for identification of three frequent, clinically significant Salmonella serovars in Kazakhstan. We determined the diagnostic efficacy of three molecular methods for detection of <i>S. enterica</i> subsp. enterica and typing <i>S.</i> Typhimurium, <i>S.</i> Enteritidis, and <i>S.</i> Virchow. A total of 137 clinical samples and 883 food samples were obtained in Almaty in 2018–2019. All tests showed high analytical specificity for detecting <i>S. enterica</i> and its corresponding serovariants (100%). The sensitivity of real-time PCR for each of the tested targets was 1–10 microbial cells and in conventional PCR 10–100 microbial cells. The trials with conventional PCR and real-time PCR had a diagnostic efficacy (DE) of 100% and 99.71%, respectively. The DE of real-time PCR and conventional PCR for detecting <i>S.</i> Enteritidis and <i>S.</i> Typhimurium was 99.90%, while the DE of conventional PCR and real-time PCR for detecting <i>S.</i> Virchow was 99.31% and 99.80%, respectively. The RAPD-PCR analysis of the genomic DNA of <i>Salmonella enterica</i> showed the genetic kinship of <i>S.</i> Enteritidis isolates, and the genetic heterogeneity of <i>S.</i> Typhimurium and <i>S.</i> Virchow isolates. Thus, the developed methods can be considered as alternatives to classical serotyping using antisera. |
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