Development and Evaluation of Alternative Methods to Identify the Three Most Common Serotypes of <i>Salmonella enterica</i> Causing Clinical Infections in Kazakhstan

In this study, we aimed to compare the performance of conventional PCR and real-time PCR assays as screening methods for identification of three frequent, clinically significant Salmonella serovars in Kazakhstan. We determined the diagnostic efficacy of three molecular methods for detection of <i...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Sabyrkhan M. Barmak, Yuriy A. Sinyavskiy, Aidar B. Berdygaliev, Turegeldy Sh. Sharmanov, Irina S. Savitskaya, Gulmira T. Sultankulova, Elena V. Zholdybayeva
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	<i>Salmonella enterica</i> <i>S.</i> Typhimurium <i>S.</i> Enteritidis <i>S.</i> Virchow PCR real-time PCR Biology (General) QH301-705.5
Acceso en línea:	https://doaj.org/article/a9dd2c478df3461fad412cecc80ff95e
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:a9dd2c478df3461fad412cecc80ff95e
record_format	dspace
spelling	oai:doaj.org-article:a9dd2c478df3461fad412cecc80ff95e2021-11-25T18:25:05ZDevelopment and Evaluation of Alternative Methods to Identify the Three Most Common Serotypes of <i>Salmonella enterica</i> Causing Clinical Infections in Kazakhstan10.3390/microorganisms91123192076-2607https://doaj.org/article/a9dd2c478df3461fad412cecc80ff95e2021-11-01T00:00:00Zhttps://www.mdpi.com/2076-2607/9/11/2319https://doaj.org/toc/2076-2607In this study, we aimed to compare the performance of conventional PCR and real-time PCR assays as screening methods for identification of three frequent, clinically significant Salmonella serovars in Kazakhstan. We determined the diagnostic efficacy of three molecular methods for detection of <i>S. enterica</i> subsp. enterica and typing <i>S.</i> Typhimurium, <i>S.</i> Enteritidis, and <i>S.</i> Virchow. A total of 137 clinical samples and 883 food samples were obtained in Almaty in 2018–2019. All tests showed high analytical specificity for detecting <i>S. enterica</i> and its corresponding serovariants (100%). The sensitivity of real-time PCR for each of the tested targets was 1–10 microbial cells and in conventional PCR 10–100 microbial cells. The trials with conventional PCR and real-time PCR had a diagnostic efficacy (DE) of 100% and 99.71%, respectively. The DE of real-time PCR and conventional PCR for detecting <i>S.</i> Enteritidis and <i>S.</i> Typhimurium was 99.90%, while the DE of conventional PCR and real-time PCR for detecting <i>S.</i> Virchow was 99.31% and 99.80%, respectively. The RAPD-PCR analysis of the genomic DNA of <i>Salmonella enterica</i> showed the genetic kinship of <i>S.</i> Enteritidis isolates, and the genetic heterogeneity of <i>S.</i> Typhimurium and <i>S.</i> Virchow isolates. Thus, the developed methods can be considered as alternatives to classical serotyping using antisera.Sabyrkhan M. BarmakYuriy A. SinyavskiyAidar B. BerdygalievTuregeldy Sh. SharmanovIrina S. SavitskayaGulmira T. SultankulovaElena V. ZholdybayevaMDPI AGarticle<i>Salmonella enterica</i><i>S.</i> Typhimurium<i>S.</i> Enteritidis<i>S.</i> VirchowPCRreal-time PCRBiology (General)QH301-705.5ENMicroorganisms, Vol 9, Iss 2319, p 2319 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	<i>Salmonella enterica</i> <i>S.</i> Typhimurium <i>S.</i> Enteritidis <i>S.</i> Virchow PCR real-time PCR Biology (General) QH301-705.5
spellingShingle	<i>Salmonella enterica</i> <i>S.</i> Typhimurium <i>S.</i> Enteritidis <i>S.</i> Virchow PCR real-time PCR Biology (General) QH301-705.5 Sabyrkhan M. Barmak Yuriy A. Sinyavskiy Aidar B. Berdygaliev Turegeldy Sh. Sharmanov Irina S. Savitskaya Gulmira T. Sultankulova Elena V. Zholdybayeva Development and Evaluation of Alternative Methods to Identify the Three Most Common Serotypes of <i>Salmonella enterica</i> Causing Clinical Infections in Kazakhstan
description	In this study, we aimed to compare the performance of conventional PCR and real-time PCR assays as screening methods for identification of three frequent, clinically significant Salmonella serovars in Kazakhstan. We determined the diagnostic efficacy of three molecular methods for detection of <i>S. enterica</i> subsp. enterica and typing <i>S.</i> Typhimurium, <i>S.</i> Enteritidis, and <i>S.</i> Virchow. A total of 137 clinical samples and 883 food samples were obtained in Almaty in 2018–2019. All tests showed high analytical specificity for detecting <i>S. enterica</i> and its corresponding serovariants (100%). The sensitivity of real-time PCR for each of the tested targets was 1–10 microbial cells and in conventional PCR 10–100 microbial cells. The trials with conventional PCR and real-time PCR had a diagnostic efficacy (DE) of 100% and 99.71%, respectively. The DE of real-time PCR and conventional PCR for detecting <i>S.</i> Enteritidis and <i>S.</i> Typhimurium was 99.90%, while the DE of conventional PCR and real-time PCR for detecting <i>S.</i> Virchow was 99.31% and 99.80%, respectively. The RAPD-PCR analysis of the genomic DNA of <i>Salmonella enterica</i> showed the genetic kinship of <i>S.</i> Enteritidis isolates, and the genetic heterogeneity of <i>S.</i> Typhimurium and <i>S.</i> Virchow isolates. Thus, the developed methods can be considered as alternatives to classical serotyping using antisera.
format	article
author	Sabyrkhan M. Barmak Yuriy A. Sinyavskiy Aidar B. Berdygaliev Turegeldy Sh. Sharmanov Irina S. Savitskaya Gulmira T. Sultankulova Elena V. Zholdybayeva
author_facet	Sabyrkhan M. Barmak Yuriy A. Sinyavskiy Aidar B. Berdygaliev Turegeldy Sh. Sharmanov Irina S. Savitskaya Gulmira T. Sultankulova Elena V. Zholdybayeva
author_sort	Sabyrkhan M. Barmak
title	Development and Evaluation of Alternative Methods to Identify the Three Most Common Serotypes of <i>Salmonella enterica</i> Causing Clinical Infections in Kazakhstan
title_short	Development and Evaluation of Alternative Methods to Identify the Three Most Common Serotypes of <i>Salmonella enterica</i> Causing Clinical Infections in Kazakhstan
title_full	Development and Evaluation of Alternative Methods to Identify the Three Most Common Serotypes of <i>Salmonella enterica</i> Causing Clinical Infections in Kazakhstan
title_fullStr	Development and Evaluation of Alternative Methods to Identify the Three Most Common Serotypes of <i>Salmonella enterica</i> Causing Clinical Infections in Kazakhstan
title_full_unstemmed	Development and Evaluation of Alternative Methods to Identify the Three Most Common Serotypes of <i>Salmonella enterica</i> Causing Clinical Infections in Kazakhstan
title_sort	development and evaluation of alternative methods to identify the three most common serotypes of <i>salmonella enterica</i> causing clinical infections in kazakhstan
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/a9dd2c478df3461fad412cecc80ff95e
work_keys_str_mv	AT sabyrkhanmbarmak developmentandevaluationofalternativemethodstoidentifythethreemostcommonserotypesofisalmonellaentericaicausingclinicalinfectionsinkazakhstan AT yuriyasinyavskiy developmentandevaluationofalternativemethodstoidentifythethreemostcommonserotypesofisalmonellaentericaicausingclinicalinfectionsinkazakhstan AT aidarbberdygaliev developmentandevaluationofalternativemethodstoidentifythethreemostcommonserotypesofisalmonellaentericaicausingclinicalinfectionsinkazakhstan AT turegeldyshsharmanov developmentandevaluationofalternativemethodstoidentifythethreemostcommonserotypesofisalmonellaentericaicausingclinicalinfectionsinkazakhstan AT irinassavitskaya developmentandevaluationofalternativemethodstoidentifythethreemostcommonserotypesofisalmonellaentericaicausingclinicalinfectionsinkazakhstan AT gulmiratsultankulova developmentandevaluationofalternativemethodstoidentifythethreemostcommonserotypesofisalmonellaentericaicausingclinicalinfectionsinkazakhstan AT elenavzholdybayeva developmentandevaluationofalternativemethodstoidentifythethreemostcommonserotypesofisalmonellaentericaicausingclinicalinfectionsinkazakhstan
_version_	1718411215781232640

Development and Evaluation of Alternative Methods to Identify the Three Most Common Serotypes of <i>Salmonella enterica</i> Causing Clinical Infections in Kazakhstan

Ejemplares similares