ROS-Mediated Apoptosis and Autophagy in Ovarian Cancer Cells Treated with Peanut-Shaped Gold Nanoparticles

Ewelina Piktel,1 Ilona Ościłowska,2 Łukasz Suprewicz,1 Joanna Depciuch,3 Natalia Marcińczyk,4 Ewa Chabielska,4 Przemysław Wolak,5 Tomasz Wollny,6 Marianna Janion,5 Magdalena Parlinska-Wojtan,3 Robert Bucki1 1Department of Medical Microbiology and Nanobiomedical Engineering, Medical University of Bia...

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Autores principales:	Piktel E, Ościłowska I, Suprewicz Ł, Depciuch J, Marcińczyk N, Chabielska E, Wolak P, Wollny T, Janion M, Parlinska-Wojtan M, Bucki R
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2021
Materias:	gold nanoparticles ovarian cancer gold nanopeanuts anti-cancer therapy nanotechnology apoptosis autophagy Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/a9dff35f4f234179bec8235828591482
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spelling	oai:doaj.org-article:a9dff35f4f234179bec82358285914822021-12-02T16:00:18ZROS-Mediated Apoptosis and Autophagy in Ovarian Cancer Cells Treated with Peanut-Shaped Gold Nanoparticles1178-2013https://doaj.org/article/a9dff35f4f234179bec82358285914822021-03-01T00:00:00Zhttps://www.dovepress.com/ros-mediated-apoptosis-and-autophagy-in-ovarian-cancer-cells-treated-w-peer-reviewed-article-IJNhttps://doaj.org/toc/1178-2013Ewelina Piktel,1 Ilona Ościłowska,2 Łukasz Suprewicz,1 Joanna Depciuch,3 Natalia Marcińczyk,4 Ewa Chabielska,4 Przemysław Wolak,5 Tomasz Wollny,6 Marianna Janion,5 Magdalena Parlinska-Wojtan,3 Robert Bucki1 1Department of Medical Microbiology and Nanobiomedical Engineering, Medical University of Bialystok, Bialystok, 15-222, Poland; 2Department of Medicinal Chemistry, Medical University of Bialystok, Bialystok, 15-222, Poland; 3Institute of Nuclear Physics Polish Academy of Sciences, Krakow, PL-31342, Poland; 4Department of Biopharmacy, Medical University of Bialystok, Bialystok, 15-222, Poland; 5 Institute of Medical Sciences, Collegium Medicum, Jan Kochanowski University, Kielce, 25-317, Poland; 6Holy Cross Cancer Center in Kielce, Kielce, 25-734, PolandCorrespondence: Robert BuckiDepartment of Medical Microbiology and Nanobiomedical Engineering, Medical University of Bialystok, Mickiewicza 2C, Bialystok, 15-222, PolandTel + 48 85 748 5793Email buckirobert@gmail.comBackground: Even with considerable improvement in treatment of epithelial ovarian cancer achieved in recent years, an increasing chemotherapy resistance and disease 5-year relapse is recorded for a majority part of patients that encourages the search for better therapeutic options. Gold nanoparticles (Au NPs) due to plethora of unique physiochemical features are thoroughly tested as drug delivery, radiosensitizers, as well as photothermal and photodynamic therapy agents. Importantly, due to highly controlled synthesis, it is possible to obtain nanomaterials with directed size and shape.Methods: In this work, we developed novel elongated-type gold nanoparticles in the shape of nanopeanuts (AuP NPs) and investigated their cytotoxic potential against ovarian cancer cells SKOV-3 using colorimetric and fluorimetric methods, Western blot, flow cytometry, and fluorescence microscopy.Results: Peanut-shaped gold nanoparticles showed high anti-cancer activity in vitro against SKOV-3 cells at doses of 1– 5 ng/mL upon 72 hours treatment. We demonstrate that AuP NPs decrease the viability and proliferation capability of ovarian cancer cells by triggering cell apoptosis and autophagy, as evidenced by flow cytometry and Western blot analyses. The overproduction of reactive oxygen species (ROS) was noted to be a critical mediator of AuP NPs-mediated cell death.Conclusion: These data indicate that gold nanopeanuts might be developed as nanotherapeutics against ovarian cancer.Keywords: gold nanoparticles, ovarian cancer, gold nanopeanuts, anti-cancer therapy, nanotechnology, apoptosis, autophagyPiktel EOściłowska ISuprewicz ŁDepciuch JMarcińczyk NChabielska EWolak PWollny TJanion MParlinska-Wojtan MBucki RDove Medical Pressarticlegold nanoparticlesovarian cancergold nanopeanutsanti-cancer therapynanotechnologyapoptosisautophagyMedicine (General)R5-920ENInternational Journal of Nanomedicine, Vol Volume 16, Pp 1993-2011 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	gold nanoparticles ovarian cancer gold nanopeanuts anti-cancer therapy nanotechnology apoptosis autophagy Medicine (General) R5-920
spellingShingle	gold nanoparticles ovarian cancer gold nanopeanuts anti-cancer therapy nanotechnology apoptosis autophagy Medicine (General) R5-920 Piktel E Ościłowska I Suprewicz Ł Depciuch J Marcińczyk N Chabielska E Wolak P Wollny T Janion M Parlinska-Wojtan M Bucki R ROS-Mediated Apoptosis and Autophagy in Ovarian Cancer Cells Treated with Peanut-Shaped Gold Nanoparticles
description	Ewelina Piktel,1 Ilona Ościłowska,2 Łukasz Suprewicz,1 Joanna Depciuch,3 Natalia Marcińczyk,4 Ewa Chabielska,4 Przemysław Wolak,5 Tomasz Wollny,6 Marianna Janion,5 Magdalena Parlinska-Wojtan,3 Robert Bucki1 1Department of Medical Microbiology and Nanobiomedical Engineering, Medical University of Bialystok, Bialystok, 15-222, Poland; 2Department of Medicinal Chemistry, Medical University of Bialystok, Bialystok, 15-222, Poland; 3Institute of Nuclear Physics Polish Academy of Sciences, Krakow, PL-31342, Poland; 4Department of Biopharmacy, Medical University of Bialystok, Bialystok, 15-222, Poland; 5 Institute of Medical Sciences, Collegium Medicum, Jan Kochanowski University, Kielce, 25-317, Poland; 6Holy Cross Cancer Center in Kielce, Kielce, 25-734, PolandCorrespondence: Robert BuckiDepartment of Medical Microbiology and Nanobiomedical Engineering, Medical University of Bialystok, Mickiewicza 2C, Bialystok, 15-222, PolandTel + 48 85 748 5793Email buckirobert@gmail.comBackground: Even with considerable improvement in treatment of epithelial ovarian cancer achieved in recent years, an increasing chemotherapy resistance and disease 5-year relapse is recorded for a majority part of patients that encourages the search for better therapeutic options. Gold nanoparticles (Au NPs) due to plethora of unique physiochemical features are thoroughly tested as drug delivery, radiosensitizers, as well as photothermal and photodynamic therapy agents. Importantly, due to highly controlled synthesis, it is possible to obtain nanomaterials with directed size and shape.Methods: In this work, we developed novel elongated-type gold nanoparticles in the shape of nanopeanuts (AuP NPs) and investigated their cytotoxic potential against ovarian cancer cells SKOV-3 using colorimetric and fluorimetric methods, Western blot, flow cytometry, and fluorescence microscopy.Results: Peanut-shaped gold nanoparticles showed high anti-cancer activity in vitro against SKOV-3 cells at doses of 1– 5 ng/mL upon 72 hours treatment. We demonstrate that AuP NPs decrease the viability and proliferation capability of ovarian cancer cells by triggering cell apoptosis and autophagy, as evidenced by flow cytometry and Western blot analyses. The overproduction of reactive oxygen species (ROS) was noted to be a critical mediator of AuP NPs-mediated cell death.Conclusion: These data indicate that gold nanopeanuts might be developed as nanotherapeutics against ovarian cancer.Keywords: gold nanoparticles, ovarian cancer, gold nanopeanuts, anti-cancer therapy, nanotechnology, apoptosis, autophagy
format	article
author	Piktel E Ościłowska I Suprewicz Ł Depciuch J Marcińczyk N Chabielska E Wolak P Wollny T Janion M Parlinska-Wojtan M Bucki R
author_facet	Piktel E Ościłowska I Suprewicz Ł Depciuch J Marcińczyk N Chabielska E Wolak P Wollny T Janion M Parlinska-Wojtan M Bucki R
author_sort	Piktel E
title	ROS-Mediated Apoptosis and Autophagy in Ovarian Cancer Cells Treated with Peanut-Shaped Gold Nanoparticles
title_short	ROS-Mediated Apoptosis and Autophagy in Ovarian Cancer Cells Treated with Peanut-Shaped Gold Nanoparticles
title_full	ROS-Mediated Apoptosis and Autophagy in Ovarian Cancer Cells Treated with Peanut-Shaped Gold Nanoparticles
title_fullStr	ROS-Mediated Apoptosis and Autophagy in Ovarian Cancer Cells Treated with Peanut-Shaped Gold Nanoparticles
title_full_unstemmed	ROS-Mediated Apoptosis and Autophagy in Ovarian Cancer Cells Treated with Peanut-Shaped Gold Nanoparticles
title_sort	ros-mediated apoptosis and autophagy in ovarian cancer cells treated with peanut-shaped gold nanoparticles
publisher	Dove Medical Press
publishDate	2021
url	https://doaj.org/article/a9dff35f4f234179bec8235828591482
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ROS-Mediated Apoptosis and Autophagy in Ovarian Cancer Cells Treated with Peanut-Shaped Gold Nanoparticles

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