Distinct pathways for zinc metabolism in the terrestrial slug Arion vulgaris

Abstract In most organisms, the concentration of free Zn2+ is controlled by metallothioneins (MTs). In contrast, no significant proportions of Zn2+ are bound to MTs in the slug, Arion vulgaris. Instead, this species possesses cytoplasmic low-molecular-weight Zn2+ (LMW Zn) binding compound that diver...

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Autores principales:	Martin Dvorak, Raimund Schnegg, Willy Salvenmoser, Òscar Palacios, Herbert Lindner, Oliver Zerbe, Armin Hansel, Markus Leiminger, Gerhard Steiner, Reinhard Dallinger, Reinhard Lackner
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2019
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/a9e6dc91279141f198376b3b970fe290
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spelling	oai:doaj.org-article:a9e6dc91279141f198376b3b970fe2902021-12-02T13:34:54ZDistinct pathways for zinc metabolism in the terrestrial slug Arion vulgaris10.1038/s41598-019-56577-72045-2322https://doaj.org/article/a9e6dc91279141f198376b3b970fe2902019-12-01T00:00:00Zhttps://doi.org/10.1038/s41598-019-56577-7https://doaj.org/toc/2045-2322Abstract In most organisms, the concentration of free Zn2+ is controlled by metallothioneins (MTs). In contrast, no significant proportions of Zn2+ are bound to MTs in the slug, Arion vulgaris. Instead, this species possesses cytoplasmic low-molecular-weight Zn2+ (LMW Zn) binding compound that divert these metal ions into pathways uncoupled from MT metabolism. Zn2+ is accumulated in the midgut gland calcium cells of Arion vulgaris, where they associate with a low-molecular-weight ligand with an apparent molecular mass of ~ 2,000 Da. Mass spectrometry of the semi-purified LMW Zn binding compound combining an electrospray ion source with a differential mobility analyser coupled to a time-of-flight mass spectrometer revealed the presence of four Zn2+-containing ion signals, which arise from disintegration of one higher MW complex resulting in an ion-mobility diameter of 1.62 nm and a molecular mass of 837 Da. We expect that the novel Zn2+ ion storage pathway may be shared by many other gastropods, and particularly species that possess Cd-selective MT isoforms or variants with only very low affinity to Zn2+.Martin DvorakRaimund SchneggWilly SalvenmoserÒscar PalaciosHerbert LindnerOliver ZerbeArmin HanselMarkus LeimingerGerhard SteinerReinhard DallingerReinhard LacknerNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 9, Iss 1, Pp 1-10 (2019)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Martin Dvorak Raimund Schnegg Willy Salvenmoser Òscar Palacios Herbert Lindner Oliver Zerbe Armin Hansel Markus Leiminger Gerhard Steiner Reinhard Dallinger Reinhard Lackner Distinct pathways for zinc metabolism in the terrestrial slug Arion vulgaris
description	Abstract In most organisms, the concentration of free Zn2+ is controlled by metallothioneins (MTs). In contrast, no significant proportions of Zn2+ are bound to MTs in the slug, Arion vulgaris. Instead, this species possesses cytoplasmic low-molecular-weight Zn2+ (LMW Zn) binding compound that divert these metal ions into pathways uncoupled from MT metabolism. Zn2+ is accumulated in the midgut gland calcium cells of Arion vulgaris, where they associate with a low-molecular-weight ligand with an apparent molecular mass of ~ 2,000 Da. Mass spectrometry of the semi-purified LMW Zn binding compound combining an electrospray ion source with a differential mobility analyser coupled to a time-of-flight mass spectrometer revealed the presence of four Zn2+-containing ion signals, which arise from disintegration of one higher MW complex resulting in an ion-mobility diameter of 1.62 nm and a molecular mass of 837 Da. We expect that the novel Zn2+ ion storage pathway may be shared by many other gastropods, and particularly species that possess Cd-selective MT isoforms or variants with only very low affinity to Zn2+.
format	article
author	Martin Dvorak Raimund Schnegg Willy Salvenmoser Òscar Palacios Herbert Lindner Oliver Zerbe Armin Hansel Markus Leiminger Gerhard Steiner Reinhard Dallinger Reinhard Lackner
author_facet	Martin Dvorak Raimund Schnegg Willy Salvenmoser Òscar Palacios Herbert Lindner Oliver Zerbe Armin Hansel Markus Leiminger Gerhard Steiner Reinhard Dallinger Reinhard Lackner
author_sort	Martin Dvorak
title	Distinct pathways for zinc metabolism in the terrestrial slug Arion vulgaris
title_short	Distinct pathways for zinc metabolism in the terrestrial slug Arion vulgaris
title_full	Distinct pathways for zinc metabolism in the terrestrial slug Arion vulgaris
title_fullStr	Distinct pathways for zinc metabolism in the terrestrial slug Arion vulgaris
title_full_unstemmed	Distinct pathways for zinc metabolism in the terrestrial slug Arion vulgaris
title_sort	distinct pathways for zinc metabolism in the terrestrial slug arion vulgaris
publisher	Nature Portfolio
publishDate	2019
url	https://doaj.org/article/a9e6dc91279141f198376b3b970fe290
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Distinct pathways for zinc metabolism in the terrestrial slug Arion vulgaris

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