Pbp1, the yeast ortholog of human Ataxin-2, functions in the cell growth on non-fermentable carbon sources.

Pbp1, the yeast ortholog of human Ataxin-2, was originally isolated as a poly(A) binding protein (Pab1)-binding protein. Pbp1 regulates the Pan2-Pan3 deadenylase complex, thereby modulating the mRNA stability and translation efficiency. However, the physiological significance of Pbp1 remains unclear...

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Autores principales:	Dang Thi Tuong Vi, Shiori Fujii, Arvin Lapiz Valderrama, Ayaka Ito, Eri Matsuura, Ayaka Nishihata, Kaoru Irie, Yasuyuki Suda, Tomoaki Mizuno, Kenji Irie
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/a9e7ebd3158546eba954f8c4a4b50c60
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spelling	oai:doaj.org-article:a9e7ebd3158546eba954f8c4a4b50c602021-12-02T20:04:05ZPbp1, the yeast ortholog of human Ataxin-2, functions in the cell growth on non-fermentable carbon sources.1932-620310.1371/journal.pone.0251456https://doaj.org/article/a9e7ebd3158546eba954f8c4a4b50c602021-01-01T00:00:00Zhttps://doi.org/10.1371/journal.pone.0251456https://doaj.org/toc/1932-6203Pbp1, the yeast ortholog of human Ataxin-2, was originally isolated as a poly(A) binding protein (Pab1)-binding protein. Pbp1 regulates the Pan2-Pan3 deadenylase complex, thereby modulating the mRNA stability and translation efficiency. However, the physiological significance of Pbp1 remains unclear since a yeast strain harboring PBP1 deletion grows similarly to wild-type strain on normal glucose-containing medium. In this study, we found that Pbp1 has a role in cell growth on the medium containing non-fermentable carbon sources. While the pbp1Δ mutant showed a similar growth compared to the wild-type cell on a normal glucose-containing medium, the pbp1Δ mutant showed a slower growth on the medium containing glycerol and lactate. Microarray analyses revealed that expressions of the genes involved in gluconeogenesis, such as PCK1 and FBP1, and of the genes involved in mitochondrial function, such as COX10 and COX11, were decreased in the pbp1Δ mutant. Pbp1 regulated the expressions of PCK1 and FBP1 via their promoters, while the expressions of COX10 and COX11 were regulated by Pbp1, not through their promoters. The decreased expressions of COX10 and COX11 in the pbp1Δ mutant were recovered by the loss of Dcp1 decapping enzyme or Xrn1 5'-3'exonuclease. Our results suggest that Pbp1 regulates the expressions of the genes involved in gluconeogenesis and mitochondrial function through multiple mechanisms.Dang Thi Tuong ViShiori FujiiArvin Lapiz ValderramaAyaka ItoEri MatsuuraAyaka NishihataKaoru IrieYasuyuki SudaTomoaki MizunoKenji IriePublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 16, Iss 5, p e0251456 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Dang Thi Tuong Vi Shiori Fujii Arvin Lapiz Valderrama Ayaka Ito Eri Matsuura Ayaka Nishihata Kaoru Irie Yasuyuki Suda Tomoaki Mizuno Kenji Irie Pbp1, the yeast ortholog of human Ataxin-2, functions in the cell growth on non-fermentable carbon sources.
description	Pbp1, the yeast ortholog of human Ataxin-2, was originally isolated as a poly(A) binding protein (Pab1)-binding protein. Pbp1 regulates the Pan2-Pan3 deadenylase complex, thereby modulating the mRNA stability and translation efficiency. However, the physiological significance of Pbp1 remains unclear since a yeast strain harboring PBP1 deletion grows similarly to wild-type strain on normal glucose-containing medium. In this study, we found that Pbp1 has a role in cell growth on the medium containing non-fermentable carbon sources. While the pbp1Δ mutant showed a similar growth compared to the wild-type cell on a normal glucose-containing medium, the pbp1Δ mutant showed a slower growth on the medium containing glycerol and lactate. Microarray analyses revealed that expressions of the genes involved in gluconeogenesis, such as PCK1 and FBP1, and of the genes involved in mitochondrial function, such as COX10 and COX11, were decreased in the pbp1Δ mutant. Pbp1 regulated the expressions of PCK1 and FBP1 via their promoters, while the expressions of COX10 and COX11 were regulated by Pbp1, not through their promoters. The decreased expressions of COX10 and COX11 in the pbp1Δ mutant were recovered by the loss of Dcp1 decapping enzyme or Xrn1 5'-3'exonuclease. Our results suggest that Pbp1 regulates the expressions of the genes involved in gluconeogenesis and mitochondrial function through multiple mechanisms.
format	article
author	Dang Thi Tuong Vi Shiori Fujii Arvin Lapiz Valderrama Ayaka Ito Eri Matsuura Ayaka Nishihata Kaoru Irie Yasuyuki Suda Tomoaki Mizuno Kenji Irie
author_facet	Dang Thi Tuong Vi Shiori Fujii Arvin Lapiz Valderrama Ayaka Ito Eri Matsuura Ayaka Nishihata Kaoru Irie Yasuyuki Suda Tomoaki Mizuno Kenji Irie
author_sort	Dang Thi Tuong Vi
title	Pbp1, the yeast ortholog of human Ataxin-2, functions in the cell growth on non-fermentable carbon sources.
title_short	Pbp1, the yeast ortholog of human Ataxin-2, functions in the cell growth on non-fermentable carbon sources.
title_full	Pbp1, the yeast ortholog of human Ataxin-2, functions in the cell growth on non-fermentable carbon sources.
title_fullStr	Pbp1, the yeast ortholog of human Ataxin-2, functions in the cell growth on non-fermentable carbon sources.
title_full_unstemmed	Pbp1, the yeast ortholog of human Ataxin-2, functions in the cell growth on non-fermentable carbon sources.
title_sort	pbp1, the yeast ortholog of human ataxin-2, functions in the cell growth on non-fermentable carbon sources.
publisher	Public Library of Science (PLoS)
publishDate	2021
url	https://doaj.org/article/a9e7ebd3158546eba954f8c4a4b50c60
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Pbp1, the yeast ortholog of human Ataxin-2, functions in the cell growth on non-fermentable carbon sources.

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