The combination of three-dimensional and rotary cell culture system promotes the proliferation and maintains the differentiation potential of rat BMSCs

The combination of three-dimensional and rotary cell culture system promotes the proliferation and maintains the differentiation potential of rat BMSCs

Abstract Bone marrow mesenchymal stem cells (BMSCs) are a good candidate for tissue engineering and clinical application. One of the challenges in its cell therapy is how to quickly obtain an adequate number of seed cells and meanwhile maintain suitable differentiation potential. In this study we co...

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Autores principales: Yilong Tang, Yan Xu, Zhifeng Xiao, Yannan Zhao, Jing Li, Sufang Han, Lei Chen, Bin Dai, Ling Wang, Bing Chen, Hong Wang
Formato: article
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Publicado: Nature Portfolio 2017
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Acceso en línea:https://doaj.org/article/a9ed7e2bb8c64343972da2e385806718
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spelling oai:doaj.org-article:a9ed7e2bb8c64343972da2e3858067182021-12-02T16:06:38ZThe combination of three-dimensional and rotary cell culture system promotes the proliferation and maintains the differentiation potential of rat BMSCs10.1038/s41598-017-00087-x2045-2322https://doaj.org/article/a9ed7e2bb8c64343972da2e3858067182017-03-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-00087-xhttps://doaj.org/toc/2045-2322Abstract Bone marrow mesenchymal stem cells (BMSCs) are a good candidate for tissue engineering and clinical application. One of the challenges in its cell therapy is how to quickly obtain an adequate number of seed cells and meanwhile maintain suitable differentiation potential. In this study we combined three-dimensional (3D) collagen porous scaffolds with rotary cell culture system (RCCS) (RCCS-3D) to create a stereoscopic dynamic environment for the amplification of rat BMSCs in vitro. The results revealed that this RCCS-3D system could enhance BMSCs’ proliferation and colony formation, as well as maintain the differentiation potential compared with conventional static two-dimensional (2D) and 3D cell culture conditions. In addition, high-throughput microarray analysis showed that gene expressions of RCCS-3D system displayed significant differences in cell proliferation and differentiation compared with static-2D conditions. Thus, RCCS-3D system could provide an effective means for BMSCs cell proliferation in vitro and meanwhile maintain differentiation potential in tissue engineering.Yilong TangYan XuZhifeng XiaoYannan ZhaoJing LiSufang HanLei ChenBin DaiLing WangBing ChenHong WangNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-15 (2017)
institution DOAJ
collection DOAJ
language EN
topic Medicine
R
Science
Q
spellingShingle Medicine
R
Science
Q
Yilong Tang
Yan Xu
Zhifeng Xiao
Yannan Zhao
Jing Li
Sufang Han
Lei Chen
Bin Dai
Ling Wang
Bing Chen
Hong Wang
The combination of three-dimensional and rotary cell culture system promotes the proliferation and maintains the differentiation potential of rat BMSCs
description Abstract Bone marrow mesenchymal stem cells (BMSCs) are a good candidate for tissue engineering and clinical application. One of the challenges in its cell therapy is how to quickly obtain an adequate number of seed cells and meanwhile maintain suitable differentiation potential. In this study we combined three-dimensional (3D) collagen porous scaffolds with rotary cell culture system (RCCS) (RCCS-3D) to create a stereoscopic dynamic environment for the amplification of rat BMSCs in vitro. The results revealed that this RCCS-3D system could enhance BMSCs’ proliferation and colony formation, as well as maintain the differentiation potential compared with conventional static two-dimensional (2D) and 3D cell culture conditions. In addition, high-throughput microarray analysis showed that gene expressions of RCCS-3D system displayed significant differences in cell proliferation and differentiation compared with static-2D conditions. Thus, RCCS-3D system could provide an effective means for BMSCs cell proliferation in vitro and meanwhile maintain differentiation potential in tissue engineering.
format article
author Yilong Tang
Yan Xu
Zhifeng Xiao
Yannan Zhao
Jing Li
Sufang Han
Lei Chen
Bin Dai
Ling Wang
Bing Chen
Hong Wang
author_facet Yilong Tang
Yan Xu
Zhifeng Xiao
Yannan Zhao
Jing Li
Sufang Han
Lei Chen
Bin Dai
Ling Wang
Bing Chen
Hong Wang
author_sort Yilong Tang
title The combination of three-dimensional and rotary cell culture system promotes the proliferation and maintains the differentiation potential of rat BMSCs
title_short The combination of three-dimensional and rotary cell culture system promotes the proliferation and maintains the differentiation potential of rat BMSCs
title_full The combination of three-dimensional and rotary cell culture system promotes the proliferation and maintains the differentiation potential of rat BMSCs
title_fullStr The combination of three-dimensional and rotary cell culture system promotes the proliferation and maintains the differentiation potential of rat BMSCs
title_full_unstemmed The combination of three-dimensional and rotary cell culture system promotes the proliferation and maintains the differentiation potential of rat BMSCs
title_sort combination of three-dimensional and rotary cell culture system promotes the proliferation and maintains the differentiation potential of rat bmscs
publisher Nature Portfolio
publishDate 2017
url https://doaj.org/article/a9ed7e2bb8c64343972da2e385806718
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