Characterization of Marine Organism Extracellular Matrix-Anchored Extracellular Vesicles and Their Biological Effect on the Alleviation of Pro-Inflammatory Cytokines

Representative marine materials such as biopolymers and bioceramics contain bioactive properties and are applied in regenerative medicine and tissue engineering. The marine organism-derived extracellular matrix (ECM), which consists of structural and functional molecules, has been studied as a bioma...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Sung-Han Jo, Seon-Hwa Kim, Changsu Kim, Sang-Hyug Park
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	marine organism extracellular matrix extracellular vesicle sea cucumber anti-inflammation Biology (General) QH301-705.5
Acceso en línea:	https://doaj.org/article/a9f05b4191b640b5a924a07d95361669
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:a9f05b4191b640b5a924a07d95361669
record_format	dspace
spelling	oai:doaj.org-article:a9f05b4191b640b5a924a07d953616692021-11-25T18:12:39ZCharacterization of Marine Organism Extracellular Matrix-Anchored Extracellular Vesicles and Their Biological Effect on the Alleviation of Pro-Inflammatory Cytokines10.3390/md191105921660-3397https://doaj.org/article/a9f05b4191b640b5a924a07d953616692021-10-01T00:00:00Zhttps://www.mdpi.com/1660-3397/19/11/592https://doaj.org/toc/1660-3397Representative marine materials such as biopolymers and bioceramics contain bioactive properties and are applied in regenerative medicine and tissue engineering. The marine organism-derived extracellular matrix (ECM), which consists of structural and functional molecules, has been studied as a biomaterial. It has been used to reconstruct tissues and improve biological functions. However, research on marine-derived extracellular vesicles (EVs) among marine functional materials is limited. Recent studies on marine-derived EVs were limited to eco-system studies using bacteria-released EVs. We aimed to expand the range of representative marine organisms such as fish, crustaceans, and echinoderms; establish the extraction process; and study the bioactivity capability of marine EVs. Results confirmed that marine organism ECM-anchored EVs (mEVs) have a similar morphology and cargos to those of EVs in land animals. To investigate physiological effects, lipopolysaccharide (LPS)-infected macrophages were treated with EVs derived from sea cucumber, fish, and shrimp. A comparison of the expression levels of inflammatory cytokine genes revealed that all types of mEVs alleviated pro-inflammatory cytokines, although to different degrees. Among them, the sea cucumber-derived EVs showed the strongest suppression ability. This study showed that research on EVs derived from various types of marine animals can lead to the development of high value-added therapeutics from discarded marine wastes.Sung-Han JoSeon-Hwa KimChangsu KimSang-Hyug ParkMDPI AGarticlemarine organismextracellular matrixextracellular vesiclesea cucumberanti-inflammationBiology (General)QH301-705.5ENMarine Drugs, Vol 19, Iss 592, p 592 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	marine organism extracellular matrix extracellular vesicle sea cucumber anti-inflammation Biology (General) QH301-705.5
spellingShingle	marine organism extracellular matrix extracellular vesicle sea cucumber anti-inflammation Biology (General) QH301-705.5 Sung-Han Jo Seon-Hwa Kim Changsu Kim Sang-Hyug Park Characterization of Marine Organism Extracellular Matrix-Anchored Extracellular Vesicles and Their Biological Effect on the Alleviation of Pro-Inflammatory Cytokines
description	Representative marine materials such as biopolymers and bioceramics contain bioactive properties and are applied in regenerative medicine and tissue engineering. The marine organism-derived extracellular matrix (ECM), which consists of structural and functional molecules, has been studied as a biomaterial. It has been used to reconstruct tissues and improve biological functions. However, research on marine-derived extracellular vesicles (EVs) among marine functional materials is limited. Recent studies on marine-derived EVs were limited to eco-system studies using bacteria-released EVs. We aimed to expand the range of representative marine organisms such as fish, crustaceans, and echinoderms; establish the extraction process; and study the bioactivity capability of marine EVs. Results confirmed that marine organism ECM-anchored EVs (mEVs) have a similar morphology and cargos to those of EVs in land animals. To investigate physiological effects, lipopolysaccharide (LPS)-infected macrophages were treated with EVs derived from sea cucumber, fish, and shrimp. A comparison of the expression levels of inflammatory cytokine genes revealed that all types of mEVs alleviated pro-inflammatory cytokines, although to different degrees. Among them, the sea cucumber-derived EVs showed the strongest suppression ability. This study showed that research on EVs derived from various types of marine animals can lead to the development of high value-added therapeutics from discarded marine wastes.
format	article
author	Sung-Han Jo Seon-Hwa Kim Changsu Kim Sang-Hyug Park
author_facet	Sung-Han Jo Seon-Hwa Kim Changsu Kim Sang-Hyug Park
author_sort	Sung-Han Jo
title	Characterization of Marine Organism Extracellular Matrix-Anchored Extracellular Vesicles and Their Biological Effect on the Alleviation of Pro-Inflammatory Cytokines
title_short	Characterization of Marine Organism Extracellular Matrix-Anchored Extracellular Vesicles and Their Biological Effect on the Alleviation of Pro-Inflammatory Cytokines
title_full	Characterization of Marine Organism Extracellular Matrix-Anchored Extracellular Vesicles and Their Biological Effect on the Alleviation of Pro-Inflammatory Cytokines
title_fullStr	Characterization of Marine Organism Extracellular Matrix-Anchored Extracellular Vesicles and Their Biological Effect on the Alleviation of Pro-Inflammatory Cytokines
title_full_unstemmed	Characterization of Marine Organism Extracellular Matrix-Anchored Extracellular Vesicles and Their Biological Effect on the Alleviation of Pro-Inflammatory Cytokines
title_sort	characterization of marine organism extracellular matrix-anchored extracellular vesicles and their biological effect on the alleviation of pro-inflammatory cytokines
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/a9f05b4191b640b5a924a07d95361669
work_keys_str_mv	AT sunghanjo characterizationofmarineorganismextracellularmatrixanchoredextracellularvesiclesandtheirbiologicaleffectonthealleviationofproinflammatorycytokines AT seonhwakim characterizationofmarineorganismextracellularmatrixanchoredextracellularvesiclesandtheirbiologicaleffectonthealleviationofproinflammatorycytokines AT changsukim characterizationofmarineorganismextracellularmatrixanchoredextracellularvesiclesandtheirbiologicaleffectonthealleviationofproinflammatorycytokines AT sanghyugpark characterizationofmarineorganismextracellularmatrixanchoredextracellularvesiclesandtheirbiologicaleffectonthealleviationofproinflammatorycytokines
_version_	1718411517786849280

Characterization of Marine Organism Extracellular Matrix-Anchored Extracellular Vesicles and Their Biological Effect on the Alleviation of Pro-Inflammatory Cytokines

Ejemplares similares