Human intelectin-1 (ITLN1) genetic variation and intestinal expression
Abstract Intelectins are ancient carbohydrate binding proteins, spanning chordate evolution and implicated in multiple human diseases. Previous GWAS have linked SNPs in ITLN1 (also known as omentin) with susceptibility to Crohn's disease (CD); however, analysis of possible functional significan...
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Nature Portfolio
2021
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oai:doaj.org-article:a9f7b8fb0d704bc08db78ccd4cacb4d32021-12-02T17:24:10ZHuman intelectin-1 (ITLN1) genetic variation and intestinal expression10.1038/s41598-021-92198-92045-2322https://doaj.org/article/a9f7b8fb0d704bc08db78ccd4cacb4d32021-06-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-92198-9https://doaj.org/toc/2045-2322Abstract Intelectins are ancient carbohydrate binding proteins, spanning chordate evolution and implicated in multiple human diseases. Previous GWAS have linked SNPs in ITLN1 (also known as omentin) with susceptibility to Crohn's disease (CD); however, analysis of possible functional significance of SNPs at this locus is lacking. Using the Ensembl database, pairwise linkage disequilibrium (LD) analyses indicated that several disease-associated SNPs at the ITLN1 locus, including SNPs in CD244 and Ly9, were in LD. The alleles comprising the risk haplotype are the major alleles in European (67%), but minor alleles in African superpopulations. Neither ITLN1 mRNA nor protein abundance in intestinal tissue, which we confirm as goblet-cell derived, was altered in the CD samples overall nor when samples were analyzed according to genotype. Moreover, the missense variant V109D does not influence ITLN1 glycan binding to the glycan β-D-galactofuranose or protein–protein oligomerization. Taken together, our data are an important step in defining the role(s) of the CD-risk haplotype by determining that risk is unlikely to be due to changes in ITLN1 carbohydrate recognition, protein oligomerization, or expression levels in intestinal mucosa. Our findings suggest that the relationship between the genomic data and disease arises from changes in CD244 or Ly9 biology, differences in ITLN1 expression in other tissues, or an alteration in ITLN1 interaction with other proteins.Eric B. NonneckePatricia A. CastilloAmanda E. DuganFaisal AlmalkiMark A. UnderwoodCarol A. De La MotteWeirong YuanWuyuan LuBo ShenMalin E. V. JohanssonLaura L. KiesslingEdward J. HolloxBo LönnerdalCharles L. BevinsNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-11 (2021) |
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Medicine R Science Q Eric B. Nonnecke Patricia A. Castillo Amanda E. Dugan Faisal Almalki Mark A. Underwood Carol A. De La Motte Weirong Yuan Wuyuan Lu Bo Shen Malin E. V. Johansson Laura L. Kiessling Edward J. Hollox Bo Lönnerdal Charles L. Bevins Human intelectin-1 (ITLN1) genetic variation and intestinal expression |
| description |
Abstract Intelectins are ancient carbohydrate binding proteins, spanning chordate evolution and implicated in multiple human diseases. Previous GWAS have linked SNPs in ITLN1 (also known as omentin) with susceptibility to Crohn's disease (CD); however, analysis of possible functional significance of SNPs at this locus is lacking. Using the Ensembl database, pairwise linkage disequilibrium (LD) analyses indicated that several disease-associated SNPs at the ITLN1 locus, including SNPs in CD244 and Ly9, were in LD. The alleles comprising the risk haplotype are the major alleles in European (67%), but minor alleles in African superpopulations. Neither ITLN1 mRNA nor protein abundance in intestinal tissue, which we confirm as goblet-cell derived, was altered in the CD samples overall nor when samples were analyzed according to genotype. Moreover, the missense variant V109D does not influence ITLN1 glycan binding to the glycan β-D-galactofuranose or protein–protein oligomerization. Taken together, our data are an important step in defining the role(s) of the CD-risk haplotype by determining that risk is unlikely to be due to changes in ITLN1 carbohydrate recognition, protein oligomerization, or expression levels in intestinal mucosa. Our findings suggest that the relationship between the genomic data and disease arises from changes in CD244 or Ly9 biology, differences in ITLN1 expression in other tissues, or an alteration in ITLN1 interaction with other proteins. |
| format |
article |
| author |
Eric B. Nonnecke Patricia A. Castillo Amanda E. Dugan Faisal Almalki Mark A. Underwood Carol A. De La Motte Weirong Yuan Wuyuan Lu Bo Shen Malin E. V. Johansson Laura L. Kiessling Edward J. Hollox Bo Lönnerdal Charles L. Bevins |
| author_facet |
Eric B. Nonnecke Patricia A. Castillo Amanda E. Dugan Faisal Almalki Mark A. Underwood Carol A. De La Motte Weirong Yuan Wuyuan Lu Bo Shen Malin E. V. Johansson Laura L. Kiessling Edward J. Hollox Bo Lönnerdal Charles L. Bevins |
| author_sort |
Eric B. Nonnecke |
| title |
Human intelectin-1 (ITLN1) genetic variation and intestinal expression |
| title_short |
Human intelectin-1 (ITLN1) genetic variation and intestinal expression |
| title_full |
Human intelectin-1 (ITLN1) genetic variation and intestinal expression |
| title_fullStr |
Human intelectin-1 (ITLN1) genetic variation and intestinal expression |
| title_full_unstemmed |
Human intelectin-1 (ITLN1) genetic variation and intestinal expression |
| title_sort |
human intelectin-1 (itln1) genetic variation and intestinal expression |
| publisher |
Nature Portfolio |
| publishDate |
2021 |
| url |
https://doaj.org/article/a9f7b8fb0d704bc08db78ccd4cacb4d3 |
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