Metagenomes from Coastal Marine Sediments Give Insights into the Ecological Role and Cellular Features of <italic toggle="yes">Loki</italic>- and <italic toggle="yes">Thorarchaeota</italic>

ABSTRACT The genomes of Asgard Archaea, a novel archaeal proposed superphylum, share an enriched repertoire of eukaryotic signature genes and thus promise to provide insights into early eukaryote evolution. However, the distribution, metabolisms, cellular structures, and ecology of the members withi...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Lokeshwaran Manoharan, Jessica A. Kozlowski, Robert W. Murdoch, Frank E. Löffler, Filipa L. Sousa, Christa Schleper
Formato:	article
Lenguaje:	EN
Publicado:	American Society for Microbiology 2019
Materias:	Archaea Lokiarchaeota Thorarchaeota ether lipids eukaryotic evolution reductive dehalogenase Microbiology QR1-502
Acceso en línea:	https://doaj.org/article/aa021bd56fb8450584934a50dc7f6ff2
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:aa021bd56fb8450584934a50dc7f6ff2
record_format	dspace
spelling	oai:doaj.org-article:aa021bd56fb8450584934a50dc7f6ff22021-11-15T15:59:42ZMetagenomes from Coastal Marine Sediments Give Insights into the Ecological Role and Cellular Features of <italic toggle="yes">Loki</italic>- and <italic toggle="yes">Thorarchaeota</italic>10.1128/mBio.02039-192150-7511https://doaj.org/article/aa021bd56fb8450584934a50dc7f6ff22019-10-01T00:00:00Zhttps://journals.asm.org/doi/10.1128/mBio.02039-19https://doaj.org/toc/2150-7511ABSTRACT The genomes of Asgard Archaea, a novel archaeal proposed superphylum, share an enriched repertoire of eukaryotic signature genes and thus promise to provide insights into early eukaryote evolution. However, the distribution, metabolisms, cellular structures, and ecology of the members within this superphylum are not well understood. Here we provide a meta-analysis of the environmental distribution of the Asgard archaea, based on available 16S rRNA gene sequences. Metagenome sequencing of samples from a salt-crusted lagoon on the Baja California Peninsula of Mexico allowed the assembly of a new Thorarchaeota and three Lokiarchaeota genomes. Comparative analyses of all known Lokiarchaeota and Thorarchaeota genomes revealed overlapping genome content, including central carbon metabolism. Members of both groups contained putative reductive dehalogenase genes, suggesting that these organisms might be able to metabolize halogenated organic compounds. Unlike the first report on Lokiarchaeota, we identified genes encoding glycerol-1-phosphate dehydrogenase in all Loki- and Thorarchaeota genomes, suggesting that these organisms are able to synthesize bona fide archaeal lipids with their characteristic glycerol stereochemistry. IMPORTANCE Microorganisms of the superphylum Asgard Archaea are considered to be the closest living prokaryotic relatives of eukaryotes (including plants and animals) and thus promise to give insights into the early evolution of more complex life forms. However, very little is known about their biology as none of the organisms has yet been cultivated in the laboratory. Here we report on the ecological distribution of Asgard Archaea and on four newly sequenced genomes of the Lokiarchaeota and Thorarchaeota lineages that give insight into possible metabolic features that might eventually help to identify these enigmatic groups of archaea in the environment and to culture them.Lokeshwaran ManoharanJessica A. KozlowskiRobert W. MurdochFrank E. LöfflerFilipa L. SousaChrista SchleperAmerican Society for MicrobiologyarticleArchaeaLokiarchaeotaThorarchaeotaether lipidseukaryotic evolutionreductive dehalogenaseMicrobiologyQR1-502ENmBio, Vol 10, Iss 5 (2019)
institution	DOAJ
collection	DOAJ
language	EN
topic	Archaea Lokiarchaeota Thorarchaeota ether lipids eukaryotic evolution reductive dehalogenase Microbiology QR1-502
spellingShingle	Archaea Lokiarchaeota Thorarchaeota ether lipids eukaryotic evolution reductive dehalogenase Microbiology QR1-502 Lokeshwaran Manoharan Jessica A. Kozlowski Robert W. Murdoch Frank E. Löffler Filipa L. Sousa Christa Schleper Metagenomes from Coastal Marine Sediments Give Insights into the Ecological Role and Cellular Features of <italic toggle="yes">Loki</italic>- and <italic toggle="yes">Thorarchaeota</italic>
description	ABSTRACT The genomes of Asgard Archaea, a novel archaeal proposed superphylum, share an enriched repertoire of eukaryotic signature genes and thus promise to provide insights into early eukaryote evolution. However, the distribution, metabolisms, cellular structures, and ecology of the members within this superphylum are not well understood. Here we provide a meta-analysis of the environmental distribution of the Asgard archaea, based on available 16S rRNA gene sequences. Metagenome sequencing of samples from a salt-crusted lagoon on the Baja California Peninsula of Mexico allowed the assembly of a new Thorarchaeota and three Lokiarchaeota genomes. Comparative analyses of all known Lokiarchaeota and Thorarchaeota genomes revealed overlapping genome content, including central carbon metabolism. Members of both groups contained putative reductive dehalogenase genes, suggesting that these organisms might be able to metabolize halogenated organic compounds. Unlike the first report on Lokiarchaeota, we identified genes encoding glycerol-1-phosphate dehydrogenase in all Loki- and Thorarchaeota genomes, suggesting that these organisms are able to synthesize bona fide archaeal lipids with their characteristic glycerol stereochemistry. IMPORTANCE Microorganisms of the superphylum Asgard Archaea are considered to be the closest living prokaryotic relatives of eukaryotes (including plants and animals) and thus promise to give insights into the early evolution of more complex life forms. However, very little is known about their biology as none of the organisms has yet been cultivated in the laboratory. Here we report on the ecological distribution of Asgard Archaea and on four newly sequenced genomes of the Lokiarchaeota and Thorarchaeota lineages that give insight into possible metabolic features that might eventually help to identify these enigmatic groups of archaea in the environment and to culture them.
format	article
author	Lokeshwaran Manoharan Jessica A. Kozlowski Robert W. Murdoch Frank E. Löffler Filipa L. Sousa Christa Schleper
author_facet	Lokeshwaran Manoharan Jessica A. Kozlowski Robert W. Murdoch Frank E. Löffler Filipa L. Sousa Christa Schleper
author_sort	Lokeshwaran Manoharan
title	Metagenomes from Coastal Marine Sediments Give Insights into the Ecological Role and Cellular Features of <italic toggle="yes">Loki</italic>- and <italic toggle="yes">Thorarchaeota</italic>
title_short	Metagenomes from Coastal Marine Sediments Give Insights into the Ecological Role and Cellular Features of <italic toggle="yes">Loki</italic>- and <italic toggle="yes">Thorarchaeota</italic>
title_full	Metagenomes from Coastal Marine Sediments Give Insights into the Ecological Role and Cellular Features of <italic toggle="yes">Loki</italic>- and <italic toggle="yes">Thorarchaeota</italic>
title_fullStr	Metagenomes from Coastal Marine Sediments Give Insights into the Ecological Role and Cellular Features of <italic toggle="yes">Loki</italic>- and <italic toggle="yes">Thorarchaeota</italic>
title_full_unstemmed	Metagenomes from Coastal Marine Sediments Give Insights into the Ecological Role and Cellular Features of <italic toggle="yes">Loki</italic>- and <italic toggle="yes">Thorarchaeota</italic>
title_sort	metagenomes from coastal marine sediments give insights into the ecological role and cellular features of <italic toggle="yes">loki</italic>- and <italic toggle="yes">thorarchaeota</italic>
publisher	American Society for Microbiology
publishDate	2019
url	https://doaj.org/article/aa021bd56fb8450584934a50dc7f6ff2
work_keys_str_mv	AT lokeshwaranmanoharan metagenomesfromcoastalmarinesedimentsgiveinsightsintotheecologicalroleandcellularfeaturesofitalictoggleyeslokiitalicanditalictoggleyesthorarchaeotaitalic AT jessicaakozlowski metagenomesfromcoastalmarinesedimentsgiveinsightsintotheecologicalroleandcellularfeaturesofitalictoggleyeslokiitalicanditalictoggleyesthorarchaeotaitalic AT robertwmurdoch metagenomesfromcoastalmarinesedimentsgiveinsightsintotheecologicalroleandcellularfeaturesofitalictoggleyeslokiitalicanditalictoggleyesthorarchaeotaitalic AT frankeloffler metagenomesfromcoastalmarinesedimentsgiveinsightsintotheecologicalroleandcellularfeaturesofitalictoggleyeslokiitalicanditalictoggleyesthorarchaeotaitalic AT filipalsousa metagenomesfromcoastalmarinesedimentsgiveinsightsintotheecologicalroleandcellularfeaturesofitalictoggleyeslokiitalicanditalictoggleyesthorarchaeotaitalic AT christaschleper metagenomesfromcoastalmarinesedimentsgiveinsightsintotheecologicalroleandcellularfeaturesofitalictoggleyeslokiitalicanditalictoggleyesthorarchaeotaitalic
_version_	1718426988083937280

Metagenomes from Coastal Marine Sediments Give Insights into the Ecological Role and Cellular Features of <italic toggle="yes">Loki</italic>- and <italic toggle="yes">Thorarchaeota</italic>

Ejemplares similares