Identification of Borrelia species after creation of an in-house MALDI-TOF MS database.

Lyme borreliosis (LB) is a multisystemic disease caused by Borrelia burgdorferi sensu lato (sl) complex transmitted to humans by Ixodes ticks. B. burgdorferi sl complex, currently comprising at least 19 genospecies, includes the main pathogenic species responsible for human disease in Europe: B. bur...

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Autores principales:	Adriana Calderaro, Chiara Gorrini, Giovanna Piccolo, Sara Montecchini, Mirko Buttrini, Sabina Rossi, Maddalena Piergianni, Maria Cristina Arcangeletti, Flora De Conto, Carlo Chezzi, Maria Cristina Medici
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2014
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/aa061fce4bc848dcbfc6842be31a8763
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spelling	oai:doaj.org-article:aa061fce4bc848dcbfc6842be31a87632021-11-18T08:32:43ZIdentification of Borrelia species after creation of an in-house MALDI-TOF MS database.1932-620310.1371/journal.pone.0088895https://doaj.org/article/aa061fce4bc848dcbfc6842be31a87632014-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/24533160/?tool=EBIhttps://doaj.org/toc/1932-6203Lyme borreliosis (LB) is a multisystemic disease caused by Borrelia burgdorferi sensu lato (sl) complex transmitted to humans by Ixodes ticks. B. burgdorferi sl complex, currently comprising at least 19 genospecies, includes the main pathogenic species responsible for human disease in Europe: B. burgdorferi sensu stricto (ss), B. afzelii, and B. garinii. In this study, for the first time, MALDI-TOF MS was applied to Borrelia spp., supplementing the existing database, limited to the species B. burgdorferi ss, B . spielmanii and B. garinii, with the species B. afzelii, in order to enable the identification of all the species potentially implicated in LB in Europe. Moreover, we supplemented the database also with B. hermsii, which is the primary cause of tick-borne relapsing fever in western North America, B. japonica, circulating in Asia, and another reference strain of B. burgdorferi ss (B31 strain). The dendrogram obtained by analyzing the protein profiles of the different Borrelia species reflected Borrelia taxonomy, showing that all the species included in the Borrelia sl complex clustered in a unique branch, while Borrelia hermsii clustered separately. In conclusion, in this study MALDI-TOF MS proved a useful tool suitable for identification of Borrelia spp. both for diagnostic purpose and epidemiological surveillance.Adriana CalderaroChiara GorriniGiovanna PiccoloSara MontecchiniMirko ButtriniSabina RossiMaddalena PiergianniMaria Cristina ArcangelettiFlora De ContoCarlo ChezziMaria Cristina MediciPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 9, Iss 2, p e88895 (2014)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Adriana Calderaro Chiara Gorrini Giovanna Piccolo Sara Montecchini Mirko Buttrini Sabina Rossi Maddalena Piergianni Maria Cristina Arcangeletti Flora De Conto Carlo Chezzi Maria Cristina Medici Identification of Borrelia species after creation of an in-house MALDI-TOF MS database.
description	Lyme borreliosis (LB) is a multisystemic disease caused by Borrelia burgdorferi sensu lato (sl) complex transmitted to humans by Ixodes ticks. B. burgdorferi sl complex, currently comprising at least 19 genospecies, includes the main pathogenic species responsible for human disease in Europe: B. burgdorferi sensu stricto (ss), B. afzelii, and B. garinii. In this study, for the first time, MALDI-TOF MS was applied to Borrelia spp., supplementing the existing database, limited to the species B. burgdorferi ss, B . spielmanii and B. garinii, with the species B. afzelii, in order to enable the identification of all the species potentially implicated in LB in Europe. Moreover, we supplemented the database also with B. hermsii, which is the primary cause of tick-borne relapsing fever in western North America, B. japonica, circulating in Asia, and another reference strain of B. burgdorferi ss (B31 strain). The dendrogram obtained by analyzing the protein profiles of the different Borrelia species reflected Borrelia taxonomy, showing that all the species included in the Borrelia sl complex clustered in a unique branch, while Borrelia hermsii clustered separately. In conclusion, in this study MALDI-TOF MS proved a useful tool suitable for identification of Borrelia spp. both for diagnostic purpose and epidemiological surveillance.
format	article
author	Adriana Calderaro Chiara Gorrini Giovanna Piccolo Sara Montecchini Mirko Buttrini Sabina Rossi Maddalena Piergianni Maria Cristina Arcangeletti Flora De Conto Carlo Chezzi Maria Cristina Medici
author_facet	Adriana Calderaro Chiara Gorrini Giovanna Piccolo Sara Montecchini Mirko Buttrini Sabina Rossi Maddalena Piergianni Maria Cristina Arcangeletti Flora De Conto Carlo Chezzi Maria Cristina Medici
author_sort	Adriana Calderaro
title	Identification of Borrelia species after creation of an in-house MALDI-TOF MS database.
title_short	Identification of Borrelia species after creation of an in-house MALDI-TOF MS database.
title_full	Identification of Borrelia species after creation of an in-house MALDI-TOF MS database.
title_fullStr	Identification of Borrelia species after creation of an in-house MALDI-TOF MS database.
title_full_unstemmed	Identification of Borrelia species after creation of an in-house MALDI-TOF MS database.
title_sort	identification of borrelia species after creation of an in-house maldi-tof ms database.
publisher	Public Library of Science (PLoS)
publishDate	2014
url	https://doaj.org/article/aa061fce4bc848dcbfc6842be31a8763
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Identification of Borrelia species after creation of an in-house MALDI-TOF MS database.

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