Differential expression of miRNAs in a human developing neuronal cell line chronically infected with Zika virus

Zika virus (ZIKV) is a serious public health concern that may lead to neurological disorders in affected individuals. The virus can be transmitted from an infected mother to her fetus, via mosquitoes, or sexually. ZIKV infections are associated with increased risk for Guillain-Barré syndrome (GBS) a...

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Autores principales:	Omar Bagasra, Narges Sadat Shamabadi, Pratima Pandey, Abdelrahman Desoky, Ewen McLean
Formato:	article
Lenguaje:	EN
Publicado:	Taylor & Francis Group 2021
Materias:	chronic infection dysregulation human neuronal progenitor cell line microrna zika virus Medicine R
Acceso en línea:	https://doaj.org/article/aa0d9d402bbe439999d5b42b837d2cc2
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spelling	oai:doaj.org-article:aa0d9d402bbe439999d5b42b837d2cc22021-11-26T11:19:48ZDifferential expression of miRNAs in a human developing neuronal cell line chronically infected with Zika virus1993-28201819-635710.1080/19932820.2021.1909902https://doaj.org/article/aa0d9d402bbe439999d5b42b837d2cc22021-01-01T00:00:00Zhttp://dx.doi.org/10.1080/19932820.2021.1909902https://doaj.org/toc/1993-2820https://doaj.org/toc/1819-6357Zika virus (ZIKV) is a serious public health concern that may lead to neurological disorders in affected individuals. The virus can be transmitted from an infected mother to her fetus, via mosquitoes, or sexually. ZIKV infections are associated with increased risk for Guillain-Barré syndrome (GBS) and congenital microcephaly in newborns infected prenatally. Dysregulations of intracellular microRNAs (miRNAs) in infected neurons have been linked to different neurological diseases. To determine the potential role of miRNAs in ZIKV infection we developed a chronically infected neuroblastoma cell line and carried out differential expression analyses of miRNAs with reference to an uninfected neuroblastoma cell line. A total of 3192miRNAs were evaluated and 389 were found to be upregulated < 2-fold and 1291 were downregulated < 2-fold. In particular, we determined that hsa-mir-431-5p, hsa-mir-3687, hsa-mir-4655-5p, hsa-mir-6071, hsa-mir-762, hsa-mir-5787, and hsa-mir-6825-3p were significantly downregulated, ranging from -5711 to -660-fold whereas, has-mir-4315, hsa-mir-5681b, hsa-mir-6511a-3p, hsa-mir-1264, hsa-mir-4418, hsa-mir-4497, hsa-mir-4485-3p, hsa-mir-4715-3p, hsa-mir-4433-3p, hsa-mir-4708-3p, hsa-mir-1973 and hsa-mir-564 were upregulated, ranging from 20-0.8-fold. We carried out target gene alignment of these miRNAs with the ZIKV genome to predict the function of the differentially expressed miRNAs and their potential impact on ZIKV pathogenesis. These miRNAs might prove useful as novel diagnostic or therapeutic markers and targets for further research on ZIKV infection and neuronal injury resulting from ZIKV infectivity in developing fetal brain neurons.Omar BagasraNarges Sadat ShamabadiPratima PandeyAbdelrahman DesokyEwen McLeanTaylor & Francis Grouparticlechronic infectiondysregulationhuman neuronal progenitor cell linemicrornazika virusMedicineRENLibyan Journal of Medicine, Vol 16, Iss 1 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	chronic infection dysregulation human neuronal progenitor cell line microrna zika virus Medicine R
spellingShingle	chronic infection dysregulation human neuronal progenitor cell line microrna zika virus Medicine R Omar Bagasra Narges Sadat Shamabadi Pratima Pandey Abdelrahman Desoky Ewen McLean Differential expression of miRNAs in a human developing neuronal cell line chronically infected with Zika virus
description	Zika virus (ZIKV) is a serious public health concern that may lead to neurological disorders in affected individuals. The virus can be transmitted from an infected mother to her fetus, via mosquitoes, or sexually. ZIKV infections are associated with increased risk for Guillain-Barré syndrome (GBS) and congenital microcephaly in newborns infected prenatally. Dysregulations of intracellular microRNAs (miRNAs) in infected neurons have been linked to different neurological diseases. To determine the potential role of miRNAs in ZIKV infection we developed a chronically infected neuroblastoma cell line and carried out differential expression analyses of miRNAs with reference to an uninfected neuroblastoma cell line. A total of 3192miRNAs were evaluated and 389 were found to be upregulated < 2-fold and 1291 were downregulated < 2-fold. In particular, we determined that hsa-mir-431-5p, hsa-mir-3687, hsa-mir-4655-5p, hsa-mir-6071, hsa-mir-762, hsa-mir-5787, and hsa-mir-6825-3p were significantly downregulated, ranging from -5711 to -660-fold whereas, has-mir-4315, hsa-mir-5681b, hsa-mir-6511a-3p, hsa-mir-1264, hsa-mir-4418, hsa-mir-4497, hsa-mir-4485-3p, hsa-mir-4715-3p, hsa-mir-4433-3p, hsa-mir-4708-3p, hsa-mir-1973 and hsa-mir-564 were upregulated, ranging from 20-0.8-fold. We carried out target gene alignment of these miRNAs with the ZIKV genome to predict the function of the differentially expressed miRNAs and their potential impact on ZIKV pathogenesis. These miRNAs might prove useful as novel diagnostic or therapeutic markers and targets for further research on ZIKV infection and neuronal injury resulting from ZIKV infectivity in developing fetal brain neurons.
format	article
author	Omar Bagasra Narges Sadat Shamabadi Pratima Pandey Abdelrahman Desoky Ewen McLean
author_facet	Omar Bagasra Narges Sadat Shamabadi Pratima Pandey Abdelrahman Desoky Ewen McLean
author_sort	Omar Bagasra
title	Differential expression of miRNAs in a human developing neuronal cell line chronically infected with Zika virus
title_short	Differential expression of miRNAs in a human developing neuronal cell line chronically infected with Zika virus
title_full	Differential expression of miRNAs in a human developing neuronal cell line chronically infected with Zika virus
title_fullStr	Differential expression of miRNAs in a human developing neuronal cell line chronically infected with Zika virus
title_full_unstemmed	Differential expression of miRNAs in a human developing neuronal cell line chronically infected with Zika virus
title_sort	differential expression of mirnas in a human developing neuronal cell line chronically infected with zika virus
publisher	Taylor & Francis Group
publishDate	2021
url	https://doaj.org/article/aa0d9d402bbe439999d5b42b837d2cc2
work_keys_str_mv	AT omarbagasra differentialexpressionofmirnasinahumandevelopingneuronalcelllinechronicallyinfectedwithzikavirus AT nargessadatshamabadi differentialexpressionofmirnasinahumandevelopingneuronalcelllinechronicallyinfectedwithzikavirus AT pratimapandey differentialexpressionofmirnasinahumandevelopingneuronalcelllinechronicallyinfectedwithzikavirus AT abdelrahmandesoky differentialexpressionofmirnasinahumandevelopingneuronalcelllinechronicallyinfectedwithzikavirus AT ewenmclean differentialexpressionofmirnasinahumandevelopingneuronalcelllinechronicallyinfectedwithzikavirus
_version_	1718409492873347072

Differential expression of miRNAs in a human developing neuronal cell line chronically infected with Zika virus

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