Epstein-Barr virus-based plasmid enables inheritable transgene expression in mouse cerebral cortex.

Continuous development of the cerebral cortex from the prenatal to postnatal period depends on neurons and glial cells, both of which are generated from neural progenitor cells (NPCs). Owing to technical limitations regarding the transfer of genes into mouse brain, the mechanisms behind the long-ter...

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Autor principal:	Tomoko Satake
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/aa131ba3f5b6421b89a782a6407d6dc4
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spelling	oai:doaj.org-article:aa131ba3f5b6421b89a782a6407d6dc42021-12-02T20:13:56ZEpstein-Barr virus-based plasmid enables inheritable transgene expression in mouse cerebral cortex.1932-620310.1371/journal.pone.0258026https://doaj.org/article/aa131ba3f5b6421b89a782a6407d6dc42021-01-01T00:00:00Zhttps://doi.org/10.1371/journal.pone.0258026https://doaj.org/toc/1932-6203Continuous development of the cerebral cortex from the prenatal to postnatal period depends on neurons and glial cells, both of which are generated from neural progenitor cells (NPCs). Owing to technical limitations regarding the transfer of genes into mouse brain, the mechanisms behind the long-term development of the cerebral cortex have not been well studied. Plasmid transfection into NPCs in embryonic mouse brains by in utero electroporation (IUE) is a widely used technique aimed at expressing transgenes in NPCs and their recent progeny neurons. Because the plasmids in NPCs are attenuated with each cell division, the transgene is not expressed in their descendants, including glial cells. The present study shows that an Epstein-Barr virus-based plasmid (EB-oriP plasmid) is helpful for studying long-term cerebral cortex development. The use of the EB-oriP plasmid for IUE allowed transgene expression even in the descendant progeny cells of adult mouse brains. Combining the EB-oriP plasmid with the shRNA expression cassette allowed examination of the genes of interest in the continuous development of the cerebral cortex. Furthermore, preferential transgene expression was achieved in combination with cell type-specific promoter-driven transgene expression. Meanwhile, introducing the EB-oriP plasmid twice into the same individual embryos during separate embryonic development stages suggested heterogeneity of NPCs. In summary, IUE using the EB-oriP plasmid is a novel option to study the long-term development of the cerebral cortex in mice.Tomoko SatakePublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 16, Iss 9, p e0258026 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Tomoko Satake Epstein-Barr virus-based plasmid enables inheritable transgene expression in mouse cerebral cortex.
description	Continuous development of the cerebral cortex from the prenatal to postnatal period depends on neurons and glial cells, both of which are generated from neural progenitor cells (NPCs). Owing to technical limitations regarding the transfer of genes into mouse brain, the mechanisms behind the long-term development of the cerebral cortex have not been well studied. Plasmid transfection into NPCs in embryonic mouse brains by in utero electroporation (IUE) is a widely used technique aimed at expressing transgenes in NPCs and their recent progeny neurons. Because the plasmids in NPCs are attenuated with each cell division, the transgene is not expressed in their descendants, including glial cells. The present study shows that an Epstein-Barr virus-based plasmid (EB-oriP plasmid) is helpful for studying long-term cerebral cortex development. The use of the EB-oriP plasmid for IUE allowed transgene expression even in the descendant progeny cells of adult mouse brains. Combining the EB-oriP plasmid with the shRNA expression cassette allowed examination of the genes of interest in the continuous development of the cerebral cortex. Furthermore, preferential transgene expression was achieved in combination with cell type-specific promoter-driven transgene expression. Meanwhile, introducing the EB-oriP plasmid twice into the same individual embryos during separate embryonic development stages suggested heterogeneity of NPCs. In summary, IUE using the EB-oriP plasmid is a novel option to study the long-term development of the cerebral cortex in mice.
format	article
author	Tomoko Satake
author_facet	Tomoko Satake
author_sort	Tomoko Satake
title	Epstein-Barr virus-based plasmid enables inheritable transgene expression in mouse cerebral cortex.
title_short	Epstein-Barr virus-based plasmid enables inheritable transgene expression in mouse cerebral cortex.
title_full	Epstein-Barr virus-based plasmid enables inheritable transgene expression in mouse cerebral cortex.
title_fullStr	Epstein-Barr virus-based plasmid enables inheritable transgene expression in mouse cerebral cortex.
title_full_unstemmed	Epstein-Barr virus-based plasmid enables inheritable transgene expression in mouse cerebral cortex.
title_sort	epstein-barr virus-based plasmid enables inheritable transgene expression in mouse cerebral cortex.
publisher	Public Library of Science (PLoS)
publishDate	2021
url	https://doaj.org/article/aa131ba3f5b6421b89a782a6407d6dc4
work_keys_str_mv	AT tomokosatake epsteinbarrvirusbasedplasmidenablesinheritabletransgeneexpressioninmousecerebralcortex
_version_	1718374727601356800

Epstein-Barr virus-based plasmid enables inheritable transgene expression in mouse cerebral cortex.

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