Increased regulatory B cells are involved in immune evasion in patients with gastric cancer

Abstract Accumulating evidence has indicated that immune regulatory cells are involved in the establishment of tumoral immune evasion. However, the role of regulatory B cells (Bregs) in this remains unclear. Here, we identified a role for Bregs in immune evasion in gastric cancer (GC) patients. The...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Yuki Murakami, Hiroaki Saito, Shota Shimizu, Yusuke Kono, Yuji Shishido, Kozo Miyatani, Tomoyuki Matsunaga, Yoji Fukumoto, Keigo Ashida, Tomohiko Sakabe, Yuji Nakayama, Yoshiyuki Fujiwara
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2019
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/aa15a0864ffe4b4f925729f8092258a8
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:aa15a0864ffe4b4f925729f8092258a8
record_format	dspace
spelling	oai:doaj.org-article:aa15a0864ffe4b4f925729f8092258a82021-12-02T15:07:54ZIncreased regulatory B cells are involved in immune evasion in patients with gastric cancer10.1038/s41598-019-49581-42045-2322https://doaj.org/article/aa15a0864ffe4b4f925729f8092258a82019-09-01T00:00:00Zhttps://doi.org/10.1038/s41598-019-49581-4https://doaj.org/toc/2045-2322Abstract Accumulating evidence has indicated that immune regulatory cells are involved in the establishment of tumoral immune evasion. However, the role of regulatory B cells (Bregs) in this remains unclear. Here, we identified a role for Bregs in immune evasion in gastric cancer (GC) patients. The frequency of peripheral Bregs was significantly higher in GC patients than in healthy controls (P = 0.0023). Moreover, the frequency of CD19+CD24hiCD27+ B cells in GC tissue was significantly higher than in peripheral blood and healthy gastric tissue. Carboxyfluorescein succinimidyl ester labeling revealed that CD19+CD24hiCD27+ B cells could suppress the proliferation of autologous CD4+ T cells. Moreover, CD19+CD24hiCD27+ B cells inhibited the production of interferon-gamma by CD4+ T cells. Double staining immunohistochemistry of interleukin-10 and CD19 revealed 5-year overall survival rates of 65.4% and 13.3% in BregLow and BregHigh groups, respectively (P < 0.0001). Multivariate analysis indicated that the frequency of Bregs was an independent prognostic indicator in GC patients. Taken together, our results show the existence of Bregs in GC tissue, and indicate that they are significantly correlated with the prognosis of GC patients.Yuki MurakamiHiroaki SaitoShota ShimizuYusuke KonoYuji ShishidoKozo MiyataniTomoyuki MatsunagaYoji FukumotoKeigo AshidaTomohiko SakabeYuji NakayamaYoshiyuki FujiwaraNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 9, Iss 1, Pp 1-9 (2019)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Yuki Murakami Hiroaki Saito Shota Shimizu Yusuke Kono Yuji Shishido Kozo Miyatani Tomoyuki Matsunaga Yoji Fukumoto Keigo Ashida Tomohiko Sakabe Yuji Nakayama Yoshiyuki Fujiwara Increased regulatory B cells are involved in immune evasion in patients with gastric cancer
description	Abstract Accumulating evidence has indicated that immune regulatory cells are involved in the establishment of tumoral immune evasion. However, the role of regulatory B cells (Bregs) in this remains unclear. Here, we identified a role for Bregs in immune evasion in gastric cancer (GC) patients. The frequency of peripheral Bregs was significantly higher in GC patients than in healthy controls (P = 0.0023). Moreover, the frequency of CD19+CD24hiCD27+ B cells in GC tissue was significantly higher than in peripheral blood and healthy gastric tissue. Carboxyfluorescein succinimidyl ester labeling revealed that CD19+CD24hiCD27+ B cells could suppress the proliferation of autologous CD4+ T cells. Moreover, CD19+CD24hiCD27+ B cells inhibited the production of interferon-gamma by CD4+ T cells. Double staining immunohistochemistry of interleukin-10 and CD19 revealed 5-year overall survival rates of 65.4% and 13.3% in BregLow and BregHigh groups, respectively (P < 0.0001). Multivariate analysis indicated that the frequency of Bregs was an independent prognostic indicator in GC patients. Taken together, our results show the existence of Bregs in GC tissue, and indicate that they are significantly correlated with the prognosis of GC patients.
format	article
author	Yuki Murakami Hiroaki Saito Shota Shimizu Yusuke Kono Yuji Shishido Kozo Miyatani Tomoyuki Matsunaga Yoji Fukumoto Keigo Ashida Tomohiko Sakabe Yuji Nakayama Yoshiyuki Fujiwara
author_facet	Yuki Murakami Hiroaki Saito Shota Shimizu Yusuke Kono Yuji Shishido Kozo Miyatani Tomoyuki Matsunaga Yoji Fukumoto Keigo Ashida Tomohiko Sakabe Yuji Nakayama Yoshiyuki Fujiwara
author_sort	Yuki Murakami
title	Increased regulatory B cells are involved in immune evasion in patients with gastric cancer
title_short	Increased regulatory B cells are involved in immune evasion in patients with gastric cancer
title_full	Increased regulatory B cells are involved in immune evasion in patients with gastric cancer
title_fullStr	Increased regulatory B cells are involved in immune evasion in patients with gastric cancer
title_full_unstemmed	Increased regulatory B cells are involved in immune evasion in patients with gastric cancer
title_sort	increased regulatory b cells are involved in immune evasion in patients with gastric cancer
publisher	Nature Portfolio
publishDate	2019
url	https://doaj.org/article/aa15a0864ffe4b4f925729f8092258a8
work_keys_str_mv	AT yukimurakami increasedregulatorybcellsareinvolvedinimmuneevasioninpatientswithgastriccancer AT hiroakisaito increasedregulatorybcellsareinvolvedinimmuneevasioninpatientswithgastriccancer AT shotashimizu increasedregulatorybcellsareinvolvedinimmuneevasioninpatientswithgastriccancer AT yusukekono increasedregulatorybcellsareinvolvedinimmuneevasioninpatientswithgastriccancer AT yujishishido increasedregulatorybcellsareinvolvedinimmuneevasioninpatientswithgastriccancer AT kozomiyatani increasedregulatorybcellsareinvolvedinimmuneevasioninpatientswithgastriccancer AT tomoyukimatsunaga increasedregulatorybcellsareinvolvedinimmuneevasioninpatientswithgastriccancer AT yojifukumoto increasedregulatorybcellsareinvolvedinimmuneevasioninpatientswithgastriccancer AT keigoashida increasedregulatorybcellsareinvolvedinimmuneevasioninpatientswithgastriccancer AT tomohikosakabe increasedregulatorybcellsareinvolvedinimmuneevasioninpatientswithgastriccancer AT yujinakayama increasedregulatorybcellsareinvolvedinimmuneevasioninpatientswithgastriccancer AT yoshiyukifujiwara increasedregulatorybcellsareinvolvedinimmuneevasioninpatientswithgastriccancer
_version_	1718388338622201856

Increased regulatory B cells are involved in immune evasion in patients with gastric cancer

Ejemplares similares