Effects of type I Diacylglycerol O-acyltransferase (DGAT1) genes on soybean (Glycine max L.) seed composition

Abstract Type I Diacylglycerol acyltransferase (DGAT1) catalyzes the final step of the biosynthesis process of triacylglycerol (TAG), the major storage lipids in plant seeds, through the esterification of diacylglycerol (DAG). To characterize the function of DGAT1 genes on the accumulation of oil an...

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Auteurs principaux:	Sepideh Torabi, Arjun Sukumaran, Sangeeta Dhaubhadel, Sarah E. Johnson, Peter LaFayette, Wayne A. Parrott, Istvan Rajcan, Milad Eskandari
Format:	article
Langue:	EN
Publié:	Nature Portfolio 2021
Sujets:	Medicine R Science Q
Accès en ligne:	https://doaj.org/article/aa183c751f494a6384b390d7c5dbea0e
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spelling	oai:doaj.org-article:aa183c751f494a6384b390d7c5dbea0e2021-12-02T10:47:55ZEffects of type I Diacylglycerol O-acyltransferase (DGAT1) genes on soybean (Glycine max L.) seed composition10.1038/s41598-021-82131-52045-2322https://doaj.org/article/aa183c751f494a6384b390d7c5dbea0e2021-01-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-82131-5https://doaj.org/toc/2045-2322Abstract Type I Diacylglycerol acyltransferase (DGAT1) catalyzes the final step of the biosynthesis process of triacylglycerol (TAG), the major storage lipids in plant seeds, through the esterification of diacylglycerol (DAG). To characterize the function of DGAT1 genes on the accumulation of oil and other seed composition traits in soybean, transgenic lines were generated via trans-acting siRNA technology, in which three DGAT1 genes (Glyma.13G106100, Glyma.09G065300, and Glyma.17G053300) were downregulated. The simultaneous downregulation of the three isoforms in transgenic lines was found to be associated with the reduction of seed oil concentrations by up to 18 mg/g (8.3%), which was correlated with increases in seed protein concentration up to 42 mg/g (11%). Additionally, the downregulations also influenced the fatty acid compositions in the seeds of transgenic lines through increasing the level of oleic acid, up to 121 mg/g (47.3%). The results of this study illustrate the importance of DGAT1 genes in determining the seed compositions in soybean through the development of new potential technology for manipulating seed quality in soybean to meet the demands for its various food and industrial applications.Sepideh TorabiArjun SukumaranSangeeta DhaubhadelSarah E. JohnsonPeter LaFayetteWayne A. ParrottIstvan RajcanMilad EskandariNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-14 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Sepideh Torabi Arjun Sukumaran Sangeeta Dhaubhadel Sarah E. Johnson Peter LaFayette Wayne A. Parrott Istvan Rajcan Milad Eskandari Effects of type I Diacylglycerol O-acyltransferase (DGAT1) genes on soybean (Glycine max L.) seed composition
description	Abstract Type I Diacylglycerol acyltransferase (DGAT1) catalyzes the final step of the biosynthesis process of triacylglycerol (TAG), the major storage lipids in plant seeds, through the esterification of diacylglycerol (DAG). To characterize the function of DGAT1 genes on the accumulation of oil and other seed composition traits in soybean, transgenic lines were generated via trans-acting siRNA technology, in which three DGAT1 genes (Glyma.13G106100, Glyma.09G065300, and Glyma.17G053300) were downregulated. The simultaneous downregulation of the three isoforms in transgenic lines was found to be associated with the reduction of seed oil concentrations by up to 18 mg/g (8.3%), which was correlated with increases in seed protein concentration up to 42 mg/g (11%). Additionally, the downregulations also influenced the fatty acid compositions in the seeds of transgenic lines through increasing the level of oleic acid, up to 121 mg/g (47.3%). The results of this study illustrate the importance of DGAT1 genes in determining the seed compositions in soybean through the development of new potential technology for manipulating seed quality in soybean to meet the demands for its various food and industrial applications.
format	article
author	Sepideh Torabi Arjun Sukumaran Sangeeta Dhaubhadel Sarah E. Johnson Peter LaFayette Wayne A. Parrott Istvan Rajcan Milad Eskandari
author_facet	Sepideh Torabi Arjun Sukumaran Sangeeta Dhaubhadel Sarah E. Johnson Peter LaFayette Wayne A. Parrott Istvan Rajcan Milad Eskandari
author_sort	Sepideh Torabi
title	Effects of type I Diacylglycerol O-acyltransferase (DGAT1) genes on soybean (Glycine max L.) seed composition
title_short	Effects of type I Diacylglycerol O-acyltransferase (DGAT1) genes on soybean (Glycine max L.) seed composition
title_full	Effects of type I Diacylglycerol O-acyltransferase (DGAT1) genes on soybean (Glycine max L.) seed composition
title_fullStr	Effects of type I Diacylglycerol O-acyltransferase (DGAT1) genes on soybean (Glycine max L.) seed composition
title_full_unstemmed	Effects of type I Diacylglycerol O-acyltransferase (DGAT1) genes on soybean (Glycine max L.) seed composition
title_sort	effects of type i diacylglycerol o-acyltransferase (dgat1) genes on soybean (glycine max l.) seed composition
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/aa183c751f494a6384b390d7c5dbea0e
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Effects of type I Diacylglycerol O-acyltransferase (DGAT1) genes on soybean (Glycine max L.) seed composition

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