The effect of curculigo orchioides (Xianmao) on kidney energy metabolism and the related mechanism in rats based on metabolomics

Abstract The Chinese materia medica Xianmao (XM) is widely used in Chinese clinics and the traditional Chinese medicine diets. Although XM is often used to study its kidney‐yang effect, the research on its effect on kidney energy metabolism and its mechanism is still relatively lacking. In this stud...

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Autores principales:	Limei Chen, Baohua Qu, Hui Wang, Hongning Liu, Yongmei Guan, Juanqing Zhou, Jiaqi Zhang
Formato:	article
Lenguaje:	EN
Publicado:	Wiley 2021
Materias:	kidney energy metabolism metabolomics RT‐PCR Xianmao Nutrition. Foods and food supply TX341-641
Acceso en línea:	https://doaj.org/article/aa1af61f25d04702a904ab3360dfd8c1
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spelling	oai:doaj.org-article:aa1af61f25d04702a904ab3360dfd8c12021-11-04T13:06:43ZThe effect of curculigo orchioides (Xianmao) on kidney energy metabolism and the related mechanism in rats based on metabolomics2048-717710.1002/fsn3.2573https://doaj.org/article/aa1af61f25d04702a904ab3360dfd8c12021-11-01T00:00:00Zhttps://doi.org/10.1002/fsn3.2573https://doaj.org/toc/2048-7177Abstract The Chinese materia medica Xianmao (XM) is widely used in Chinese clinics and the traditional Chinese medicine diets. Although XM is often used to study its kidney‐yang effect, the research on its effect on kidney energy metabolism and its mechanism is still relatively lacking. In this study, rats were given different doses of XM water extract for 4 weeks. Biochemical method was used to detect the content of serum biochemical indexes of liver and kidney function and blood lipid indicators, and HE staining method was used to observe the histopathological of liver and kidney in rats. The kidney Na+‐K+‐ATPase, Ca2+‐Mg2+‐ATPase, SDH (succinate dehydrogenase) enzyme activity, and the content of ATP in rats were measured. Metabolomics technology was used to analyze the potential biomarkers related to the effects of XM on kidney energy metabolism, and then, the metabolic pathways were analyzed. RT‐PCR was used to detect the expression of Ampk, Sirt1, Ppar‐α, and Pgc‐1α mRNA in kidney of rats. The results showed, compared with the blank control group, there was no significant effect on liver and kidney function in XMH, XMM, and XML groups. These significantly increased the kidney Na+‐K+‐ATPase, Ca2+‐Mg2+‐ATPase, SDH enzyme activity, and ATP content in XMH, XMM, and XML groups. Mitochondrial metabolic rate was inhibited in XMH group, but it was significantly increased in XMM and XML groups. The number of mitochondria was increased in XMH, XMM, and XML groups. Overall, these effects may be mediated by TCA cycle metabolism, butanoate metabolism, propanoate metabolism, alanine, aspartate, and glutamate metabolism, retinol metabolism, purine metabolism, pentose phosphate metabolism, aminoacyl‐tRNA biosynthesis, valine, leucine, and isoleucine biosynthesis, and degradation metabolism pathways, as well as by increasing expression of upstream genes Ampk, Sirt1, Ppar‐α, and Pgc‐1α mRNA.Limei ChenBaohua QuHui WangHongning LiuYongmei GuanJuanqing ZhouJiaqi ZhangWileyarticlekidney energy metabolismmetabolomicsRT‐PCRXianmaoNutrition. Foods and food supplyTX341-641ENFood Science & Nutrition, Vol 9, Iss 11, Pp 6194-6212 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	kidney energy metabolism metabolomics RT‐PCR Xianmao Nutrition. Foods and food supply TX341-641
spellingShingle	kidney energy metabolism metabolomics RT‐PCR Xianmao Nutrition. Foods and food supply TX341-641 Limei Chen Baohua Qu Hui Wang Hongning Liu Yongmei Guan Juanqing Zhou Jiaqi Zhang The effect of curculigo orchioides (Xianmao) on kidney energy metabolism and the related mechanism in rats based on metabolomics
description	Abstract The Chinese materia medica Xianmao (XM) is widely used in Chinese clinics and the traditional Chinese medicine diets. Although XM is often used to study its kidney‐yang effect, the research on its effect on kidney energy metabolism and its mechanism is still relatively lacking. In this study, rats were given different doses of XM water extract for 4 weeks. Biochemical method was used to detect the content of serum biochemical indexes of liver and kidney function and blood lipid indicators, and HE staining method was used to observe the histopathological of liver and kidney in rats. The kidney Na+‐K+‐ATPase, Ca2+‐Mg2+‐ATPase, SDH (succinate dehydrogenase) enzyme activity, and the content of ATP in rats were measured. Metabolomics technology was used to analyze the potential biomarkers related to the effects of XM on kidney energy metabolism, and then, the metabolic pathways were analyzed. RT‐PCR was used to detect the expression of Ampk, Sirt1, Ppar‐α, and Pgc‐1α mRNA in kidney of rats. The results showed, compared with the blank control group, there was no significant effect on liver and kidney function in XMH, XMM, and XML groups. These significantly increased the kidney Na+‐K+‐ATPase, Ca2+‐Mg2+‐ATPase, SDH enzyme activity, and ATP content in XMH, XMM, and XML groups. Mitochondrial metabolic rate was inhibited in XMH group, but it was significantly increased in XMM and XML groups. The number of mitochondria was increased in XMH, XMM, and XML groups. Overall, these effects may be mediated by TCA cycle metabolism, butanoate metabolism, propanoate metabolism, alanine, aspartate, and glutamate metabolism, retinol metabolism, purine metabolism, pentose phosphate metabolism, aminoacyl‐tRNA biosynthesis, valine, leucine, and isoleucine biosynthesis, and degradation metabolism pathways, as well as by increasing expression of upstream genes Ampk, Sirt1, Ppar‐α, and Pgc‐1α mRNA.
format	article
author	Limei Chen Baohua Qu Hui Wang Hongning Liu Yongmei Guan Juanqing Zhou Jiaqi Zhang
author_facet	Limei Chen Baohua Qu Hui Wang Hongning Liu Yongmei Guan Juanqing Zhou Jiaqi Zhang
author_sort	Limei Chen
title	The effect of curculigo orchioides (Xianmao) on kidney energy metabolism and the related mechanism in rats based on metabolomics
title_short	The effect of curculigo orchioides (Xianmao) on kidney energy metabolism and the related mechanism in rats based on metabolomics
title_full	The effect of curculigo orchioides (Xianmao) on kidney energy metabolism and the related mechanism in rats based on metabolomics
title_fullStr	The effect of curculigo orchioides (Xianmao) on kidney energy metabolism and the related mechanism in rats based on metabolomics
title_full_unstemmed	The effect of curculigo orchioides (Xianmao) on kidney energy metabolism and the related mechanism in rats based on metabolomics
title_sort	effect of curculigo orchioides (xianmao) on kidney energy metabolism and the related mechanism in rats based on metabolomics
publisher	Wiley
publishDate	2021
url	https://doaj.org/article/aa1af61f25d04702a904ab3360dfd8c1
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The effect of curculigo orchioides (Xianmao) on kidney energy metabolism and the related mechanism in rats based on metabolomics

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