An Engineered Thermostable Laccase with Great Ability to Decolorize and Detoxify Malachite Green
Laccases can catalyze the remediation of hazardous synthetic dyes in an eco-friendly manner, and thermostable laccases are advantageous to treat high-temperature dyeing wastewater. A novel laccase from <i>Geothermobacter hydrogeniphilus</i> (Ghlac) was cloned and expressed in <i>Es...
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2021
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oai:doaj.org-article:aa1b648a657643cabeaa6b07c0d097b32021-11-11T17:12:41ZAn Engineered Thermostable Laccase with Great Ability to Decolorize and Detoxify Malachite Green10.3390/ijms2221117551422-00671661-6596https://doaj.org/article/aa1b648a657643cabeaa6b07c0d097b32021-10-01T00:00:00Zhttps://www.mdpi.com/1422-0067/22/21/11755https://doaj.org/toc/1661-6596https://doaj.org/toc/1422-0067Laccases can catalyze the remediation of hazardous synthetic dyes in an eco-friendly manner, and thermostable laccases are advantageous to treat high-temperature dyeing wastewater. A novel laccase from <i>Geothermobacter hydrogeniphilus</i> (Ghlac) was cloned and expressed in <i>Escherichia coli</i>. Ghlac containing 263 residues was characterized as a functional laccase of the DUF152 family. By structural and biochemical analyses, the conserved residues H78, C119, and H136 were identified to bind with one copper atom to fulfill the laccase activity. In order to make it more suitable for industrial use, Ghlac variant Mut2 with enhanced thermostability was designed. The half-lives of Mut2 at 50 °C and 60 °C were 80.6 h and 9.8 h, respectively. Mut2 was stable at pH values ranging from 4.0 to 8.0 and showed a high tolerance for organic solvents such as ethanol, acetone, and dimethyl sulfoxide. In addition, Mut2 decolorized approximately 100% of 100 mg/L of malachite green dye in 3 h at 70 °C. Furthermore, Mut2 eliminated the toxicity of malachite green to bacteria and <i>Zea mays</i>. In summary, the thermostable laccase Ghlac Mut2 could effectively decolorize and detoxify malachite green at high temperatures, showing great potential to remediate the dyeing wastewater.Guotao MaoKai WangFangyuan WangHao LiHongsen ZhangHui XieZhimin WangFengqin WangAndong SongMDPI AGarticlethermostable laccasemalachite greendecolorizationdetoxificationBiology (General)QH301-705.5ChemistryQD1-999ENInternational Journal of Molecular Sciences, Vol 22, Iss 11755, p 11755 (2021) |
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DOAJ |
| collection |
DOAJ |
| language |
EN |
| topic |
thermostable laccase malachite green decolorization detoxification Biology (General) QH301-705.5 Chemistry QD1-999 |
| spellingShingle |
thermostable laccase malachite green decolorization detoxification Biology (General) QH301-705.5 Chemistry QD1-999 Guotao Mao Kai Wang Fangyuan Wang Hao Li Hongsen Zhang Hui Xie Zhimin Wang Fengqin Wang Andong Song An Engineered Thermostable Laccase with Great Ability to Decolorize and Detoxify Malachite Green |
| description |
Laccases can catalyze the remediation of hazardous synthetic dyes in an eco-friendly manner, and thermostable laccases are advantageous to treat high-temperature dyeing wastewater. A novel laccase from <i>Geothermobacter hydrogeniphilus</i> (Ghlac) was cloned and expressed in <i>Escherichia coli</i>. Ghlac containing 263 residues was characterized as a functional laccase of the DUF152 family. By structural and biochemical analyses, the conserved residues H78, C119, and H136 were identified to bind with one copper atom to fulfill the laccase activity. In order to make it more suitable for industrial use, Ghlac variant Mut2 with enhanced thermostability was designed. The half-lives of Mut2 at 50 °C and 60 °C were 80.6 h and 9.8 h, respectively. Mut2 was stable at pH values ranging from 4.0 to 8.0 and showed a high tolerance for organic solvents such as ethanol, acetone, and dimethyl sulfoxide. In addition, Mut2 decolorized approximately 100% of 100 mg/L of malachite green dye in 3 h at 70 °C. Furthermore, Mut2 eliminated the toxicity of malachite green to bacteria and <i>Zea mays</i>. In summary, the thermostable laccase Ghlac Mut2 could effectively decolorize and detoxify malachite green at high temperatures, showing great potential to remediate the dyeing wastewater. |
| format |
article |
| author |
Guotao Mao Kai Wang Fangyuan Wang Hao Li Hongsen Zhang Hui Xie Zhimin Wang Fengqin Wang Andong Song |
| author_facet |
Guotao Mao Kai Wang Fangyuan Wang Hao Li Hongsen Zhang Hui Xie Zhimin Wang Fengqin Wang Andong Song |
| author_sort |
Guotao Mao |
| title |
An Engineered Thermostable Laccase with Great Ability to Decolorize and Detoxify Malachite Green |
| title_short |
An Engineered Thermostable Laccase with Great Ability to Decolorize and Detoxify Malachite Green |
| title_full |
An Engineered Thermostable Laccase with Great Ability to Decolorize and Detoxify Malachite Green |
| title_fullStr |
An Engineered Thermostable Laccase with Great Ability to Decolorize and Detoxify Malachite Green |
| title_full_unstemmed |
An Engineered Thermostable Laccase with Great Ability to Decolorize and Detoxify Malachite Green |
| title_sort |
engineered thermostable laccase with great ability to decolorize and detoxify malachite green |
| publisher |
MDPI AG |
| publishDate |
2021 |
| url |
https://doaj.org/article/aa1b648a657643cabeaa6b07c0d097b3 |
| work_keys_str_mv |
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