CRISPR-Based Genetic Switches and Other Complex Circuits: Research and Application

CRISPR-based enzymes have offered a unique capability to the design of genetic switches, with advantages in designability, modularity and orthogonality. CRISPR-based genetic switches operate on multiple levels of life, including transcription and translation. In both prokaryotic and eukaryotic cells...

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Auteurs principaux:	Pei Du, Chunbo Lou, Xuejin Zhao, Qihui Wang, Xiangyu Ji, Weijia Wei
Format:	article
Langue:	EN
Publié:	MDPI AG 2021
Sujets:	CRISPR genetic switch transcription translation genetic circuit Science Q
Accès en ligne:	https://doaj.org/article/aa22db21823b49df9016c2c992666094
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id	oai:doaj.org-article:aa22db21823b49df9016c2c992666094
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spelling	oai:doaj.org-article:aa22db21823b49df9016c2c9926660942021-11-25T18:11:37ZCRISPR-Based Genetic Switches and Other Complex Circuits: Research and Application10.3390/life111112552075-1729https://doaj.org/article/aa22db21823b49df9016c2c9926660942021-11-01T00:00:00Zhttps://www.mdpi.com/2075-1729/11/11/1255https://doaj.org/toc/2075-1729CRISPR-based enzymes have offered a unique capability to the design of genetic switches, with advantages in designability, modularity and orthogonality. CRISPR-based genetic switches operate on multiple levels of life, including transcription and translation. In both prokaryotic and eukaryotic cells, deactivated CRISPR endonuclease and endoribonuclease have served in genetic switches for activating or repressing gene expression, at both transcriptional and translational levels. With these genetic switches, more complex circuits have been assembled to achieve sophisticated functions including inducible switches, non-linear response and logical biocomputation. As more CRISPR enzymes continue to be excavated, CRISPR-based genetic switches will be used in a much wider range of applications.Pei DuChunbo LouXuejin ZhaoQihui WangXiangyu JiWeijia WeiMDPI AGarticleCRISPRgenetic switchtranscriptiontranslationgenetic circuitScienceQENLife, Vol 11, Iss 1255, p 1255 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	CRISPR genetic switch transcription translation genetic circuit Science Q
spellingShingle	CRISPR genetic switch transcription translation genetic circuit Science Q Pei Du Chunbo Lou Xuejin Zhao Qihui Wang Xiangyu Ji Weijia Wei CRISPR-Based Genetic Switches and Other Complex Circuits: Research and Application
description	CRISPR-based enzymes have offered a unique capability to the design of genetic switches, with advantages in designability, modularity and orthogonality. CRISPR-based genetic switches operate on multiple levels of life, including transcription and translation. In both prokaryotic and eukaryotic cells, deactivated CRISPR endonuclease and endoribonuclease have served in genetic switches for activating or repressing gene expression, at both transcriptional and translational levels. With these genetic switches, more complex circuits have been assembled to achieve sophisticated functions including inducible switches, non-linear response and logical biocomputation. As more CRISPR enzymes continue to be excavated, CRISPR-based genetic switches will be used in a much wider range of applications.
format	article
author	Pei Du Chunbo Lou Xuejin Zhao Qihui Wang Xiangyu Ji Weijia Wei
author_facet	Pei Du Chunbo Lou Xuejin Zhao Qihui Wang Xiangyu Ji Weijia Wei
author_sort	Pei Du
title	CRISPR-Based Genetic Switches and Other Complex Circuits: Research and Application
title_short	CRISPR-Based Genetic Switches and Other Complex Circuits: Research and Application
title_full	CRISPR-Based Genetic Switches and Other Complex Circuits: Research and Application
title_fullStr	CRISPR-Based Genetic Switches and Other Complex Circuits: Research and Application
title_full_unstemmed	CRISPR-Based Genetic Switches and Other Complex Circuits: Research and Application
title_sort	crispr-based genetic switches and other complex circuits: research and application
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/aa22db21823b49df9016c2c992666094
work_keys_str_mv	AT peidu crisprbasedgeneticswitchesandothercomplexcircuitsresearchandapplication AT chunbolou crisprbasedgeneticswitchesandothercomplexcircuitsresearchandapplication AT xuejinzhao crisprbasedgeneticswitchesandothercomplexcircuitsresearchandapplication AT qihuiwang crisprbasedgeneticswitchesandothercomplexcircuitsresearchandapplication AT xiangyuji crisprbasedgeneticswitchesandothercomplexcircuitsresearchandapplication AT weijiawei crisprbasedgeneticswitchesandothercomplexcircuitsresearchandapplication
_version_	1718411518006001664

CRISPR-Based Genetic Switches and Other Complex Circuits: Research and Application

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