Simultaneous Detection of VEGF and CEA by Time-Resolved Chemiluminescence Enzyme-Linked Aptamer Assay
Jin Man,1 Jiajia Dong,2 Yilin Wang,1 Leiliang He,1 Songcheng Yu,1 Fei Yu,1 Jia Wang,1 Yongmei Tian,1 Lie Liu,1 Runping Han,2 Hongchao Guo,1 Yongjun Wu,1,3 Lingbo Qu2,4 1College of Public Health, Zhengzhou University, Zhengzhou, Henan 450001, People’s Republic of China; 2College of Chemistr...
Guardado en:
| Autores principales: | , , , , , , , , , , , , |
|---|---|
| Formato: | article |
| Lenguaje: | EN |
| Publicado: |
Dove Medical Press
2020
|
| Materias: | |
| Acceso en línea: | https://doaj.org/article/aa2657fc03474906acee758925fcf083 |
| Etiquetas: |
Agregar Etiqueta
Sin Etiquetas, Sea el primero en etiquetar este registro!
|
| id |
oai:doaj.org-article:aa2657fc03474906acee758925fcf083 |
|---|---|
| record_format |
dspace |
| spelling |
oai:doaj.org-article:aa2657fc03474906acee758925fcf0832021-12-02T14:00:26ZSimultaneous Detection of VEGF and CEA by Time-Resolved Chemiluminescence Enzyme-Linked Aptamer Assay1178-2013https://doaj.org/article/aa2657fc03474906acee758925fcf0832020-12-01T00:00:00Zhttps://www.dovepress.com/simultaneous-detection-of-vegf-and-cea-by-time-resolved-chemiluminesce-peer-reviewed-article-IJNhttps://doaj.org/toc/1178-2013Jin Man,1 Jiajia Dong,2 Yilin Wang,1 Leiliang He,1 Songcheng Yu,1 Fei Yu,1 Jia Wang,1 Yongmei Tian,1 Lie Liu,1 Runping Han,2 Hongchao Guo,1 Yongjun Wu,1,3 Lingbo Qu2,4 1College of Public Health, Zhengzhou University, Zhengzhou, Henan 450001, People’s Republic of China; 2College of Chemistry, Zhengzhou University, Zhengzhou, Henan 450001, People’s Republic of China; 3The Key Laboratory of Nanomedicine and Health Inspection of Zhengzhou, Zhengzhou, Henan 450001, People’s Republic of China; 4Henan Joint International Research Laboratory of Green Construction of Functional Molecules and Their Bioanalytical Applications, Zhengzhou, Henan 450001, People’s Republic of ChinaCorrespondence: Yongjun WuCollege of Public Health, Zhengzhou University, No.100 Science Avenue, Zhengzhou City 450001, People’s Republic of ChinaTel/Fax +86-371-67781450Email wuyongjun135@126.comLingbo QuCollege of Chemistry, Zhengzhou University, No. 100 Science Avenue, Zhengzhou City 450001, People’s Republic of ChinaTel/Fax +86-371-67781450Email qulingbo@zzu.edu.cnBackground: As two important tumor markers, vascular endothelial growth factor (VEGF) and carcinoembryonic antigen (CEA) have a great value for clinical application in the early diagnosis of cancer. Due to the complex composition of biological samples, the results from combined detection of CEA and VEGF are often taken as a comprehensive indicator in order to make an accurate judgment on a disease. However, most of the current methods can only be used to detect the content of one biomarker. Therefore, it is necessary to explore a simple, rapid, low-cost, and highly sensitive method for the simultaneous detection of CEA and VEGF.Methods: Based on specific aptamers and magnetic separation, a time-resolved chemiluminescence enzyme-linked aptamer assay was developed for the simultaneous detections of CEA and VEGF in serum samples.Results: Under the optimal conditions, the linear range of the calibration curve for VEGF was from 0.5 to 80 ng mL− 1, and the limit of detection was 0.1 ng mL− 1. The linear range of the calibration curve for CEA was 0.5 to 160 ng mL− 1, and the limit of detection was 0.1 ng mL− 1. The established method was applied to detect VEGF and CEA in serum samples. The results were consistent with those of commercial kits.Conclusion: The method has high sensitivity and can quickly obtain accurate results, which could greatly improve the measurement efficiency, reduce the cost, and also reduce the volume of sample consumed. It can be seen that the method established in this study has important application value and broad application prospect in clinical diagnosis.Keywords: chemiluminescence assay, simultaneous detection, vascular endothelial growth factor, time-resolved, carcinoembryonic antigenMan JDong JWang YHe LYu SYu FWang JTian YLiu LHan RGuo HWu YQu LDove Medical Pressarticlechemiluminescence assaysimultaneous detectionvascular endothelial growth factortime-resolvedcarcinoembryonic antigenMedicine (General)R5-920ENInternational Journal of Nanomedicine, Vol Volume 15, Pp 9975-9985 (2020) |
| institution |
DOAJ |
| collection |
DOAJ |
| language |
EN |
| topic |
chemiluminescence assay simultaneous detection vascular endothelial growth factor time-resolved carcinoembryonic antigen Medicine (General) R5-920 |
| spellingShingle |
chemiluminescence assay simultaneous detection vascular endothelial growth factor time-resolved carcinoembryonic antigen Medicine (General) R5-920 Man J Dong J Wang Y He L Yu S Yu F Wang J Tian Y Liu L Han R Guo H Wu Y Qu L Simultaneous Detection of VEGF and CEA by Time-Resolved Chemiluminescence Enzyme-Linked Aptamer Assay |
| description |
Jin Man,1 Jiajia Dong,2 Yilin Wang,1 Leiliang He,1 Songcheng Yu,1 Fei Yu,1 Jia Wang,1 Yongmei Tian,1 Lie Liu,1 Runping Han,2 Hongchao Guo,1 Yongjun Wu,1,3 Lingbo Qu2,4 1College of Public Health, Zhengzhou University, Zhengzhou, Henan 450001, People’s Republic of China; 2College of Chemistry, Zhengzhou University, Zhengzhou, Henan 450001, People’s Republic of China; 3The Key Laboratory of Nanomedicine and Health Inspection of Zhengzhou, Zhengzhou, Henan 450001, People’s Republic of China; 4Henan Joint International Research Laboratory of Green Construction of Functional Molecules and Their Bioanalytical Applications, Zhengzhou, Henan 450001, People’s Republic of ChinaCorrespondence: Yongjun WuCollege of Public Health, Zhengzhou University, No.100 Science Avenue, Zhengzhou City 450001, People’s Republic of ChinaTel/Fax +86-371-67781450Email wuyongjun135@126.comLingbo QuCollege of Chemistry, Zhengzhou University, No. 100 Science Avenue, Zhengzhou City 450001, People’s Republic of ChinaTel/Fax +86-371-67781450Email qulingbo@zzu.edu.cnBackground: As two important tumor markers, vascular endothelial growth factor (VEGF) and carcinoembryonic antigen (CEA) have a great value for clinical application in the early diagnosis of cancer. Due to the complex composition of biological samples, the results from combined detection of CEA and VEGF are often taken as a comprehensive indicator in order to make an accurate judgment on a disease. However, most of the current methods can only be used to detect the content of one biomarker. Therefore, it is necessary to explore a simple, rapid, low-cost, and highly sensitive method for the simultaneous detection of CEA and VEGF.Methods: Based on specific aptamers and magnetic separation, a time-resolved chemiluminescence enzyme-linked aptamer assay was developed for the simultaneous detections of CEA and VEGF in serum samples.Results: Under the optimal conditions, the linear range of the calibration curve for VEGF was from 0.5 to 80 ng mL− 1, and the limit of detection was 0.1 ng mL− 1. The linear range of the calibration curve for CEA was 0.5 to 160 ng mL− 1, and the limit of detection was 0.1 ng mL− 1. The established method was applied to detect VEGF and CEA in serum samples. The results were consistent with those of commercial kits.Conclusion: The method has high sensitivity and can quickly obtain accurate results, which could greatly improve the measurement efficiency, reduce the cost, and also reduce the volume of sample consumed. It can be seen that the method established in this study has important application value and broad application prospect in clinical diagnosis.Keywords: chemiluminescence assay, simultaneous detection, vascular endothelial growth factor, time-resolved, carcinoembryonic antigen |
| format |
article |
| author |
Man J Dong J Wang Y He L Yu S Yu F Wang J Tian Y Liu L Han R Guo H Wu Y Qu L |
| author_facet |
Man J Dong J Wang Y He L Yu S Yu F Wang J Tian Y Liu L Han R Guo H Wu Y Qu L |
| author_sort |
Man J |
| title |
Simultaneous Detection of VEGF and CEA by Time-Resolved Chemiluminescence Enzyme-Linked Aptamer Assay |
| title_short |
Simultaneous Detection of VEGF and CEA by Time-Resolved Chemiluminescence Enzyme-Linked Aptamer Assay |
| title_full |
Simultaneous Detection of VEGF and CEA by Time-Resolved Chemiluminescence Enzyme-Linked Aptamer Assay |
| title_fullStr |
Simultaneous Detection of VEGF and CEA by Time-Resolved Chemiluminescence Enzyme-Linked Aptamer Assay |
| title_full_unstemmed |
Simultaneous Detection of VEGF and CEA by Time-Resolved Chemiluminescence Enzyme-Linked Aptamer Assay |
| title_sort |
simultaneous detection of vegf and cea by time-resolved chemiluminescence enzyme-linked aptamer assay |
| publisher |
Dove Medical Press |
| publishDate |
2020 |
| url |
https://doaj.org/article/aa2657fc03474906acee758925fcf083 |
| work_keys_str_mv |
AT manj simultaneousdetectionofvegfandceabytimeresolvedchemiluminescenceenzymelinkedaptamerassay AT dongj simultaneousdetectionofvegfandceabytimeresolvedchemiluminescenceenzymelinkedaptamerassay AT wangy simultaneousdetectionofvegfandceabytimeresolvedchemiluminescenceenzymelinkedaptamerassay AT hel simultaneousdetectionofvegfandceabytimeresolvedchemiluminescenceenzymelinkedaptamerassay AT yus simultaneousdetectionofvegfandceabytimeresolvedchemiluminescenceenzymelinkedaptamerassay AT yuf simultaneousdetectionofvegfandceabytimeresolvedchemiluminescenceenzymelinkedaptamerassay AT wangj simultaneousdetectionofvegfandceabytimeresolvedchemiluminescenceenzymelinkedaptamerassay AT tiany simultaneousdetectionofvegfandceabytimeresolvedchemiluminescenceenzymelinkedaptamerassay AT liul simultaneousdetectionofvegfandceabytimeresolvedchemiluminescenceenzymelinkedaptamerassay AT hanr simultaneousdetectionofvegfandceabytimeresolvedchemiluminescenceenzymelinkedaptamerassay AT guoh simultaneousdetectionofvegfandceabytimeresolvedchemiluminescenceenzymelinkedaptamerassay AT wuy simultaneousdetectionofvegfandceabytimeresolvedchemiluminescenceenzymelinkedaptamerassay AT qul simultaneousdetectionofvegfandceabytimeresolvedchemiluminescenceenzymelinkedaptamerassay |
| _version_ |
1718392219929411584 |