Combined Effects of Proton Radiation and Simulated Microgravity on the Cell Viability and ALP Activity of Murine Osteoblast Cells

Proton radiation (PR) and microgravity (μG) are two key factors that impact living things in space. This study aimed to explore the combined effects of PR and simulated μG (SμG) on bone function. Mouse embryo osteoblast precursor cells (MC3T3-E1) were irradiated with proton beams and immediately tre...

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Autores principales:	Liqiu Ma, Fuquan Kong, Yihao Gong, Qiaojuan Wang, Jiancheng Liu, Li Sui
Formato:	article
Lenguaje:	EN
Publicado:	Frontiers Media S.A. 2021
Materias:	proton radiation microgravity bone function transcriptome combined effects Public aspects of medicine RA1-1270
Acceso en línea:	https://doaj.org/article/aa3ea4a2dea942fdb7d9075c92084afc
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spelling	oai:doaj.org-article:aa3ea4a2dea942fdb7d9075c92084afc2021-12-01T16:07:00ZCombined Effects of Proton Radiation and Simulated Microgravity on the Cell Viability and ALP Activity of Murine Osteoblast Cells2296-256510.3389/fpubh.2021.759236https://doaj.org/article/aa3ea4a2dea942fdb7d9075c92084afc2021-11-01T00:00:00Zhttps://www.frontiersin.org/articles/10.3389/fpubh.2021.759236/fullhttps://doaj.org/toc/2296-2565Proton radiation (PR) and microgravity (μG) are two key factors that impact living things in space. This study aimed to explore the combined effects of PR and simulated μG (SμG) on bone function. Mouse embryo osteoblast precursor cells (MC3T3-E1) were irradiated with proton beams and immediately treated with SμG for 2 days using a three-dimensional clinostat. All samples were subjected to cell viability, alkaline phosphatase (ALP) activity and transcriptome assays. The results showed that cell viability decreased with increasing doses of PR. The peak ALP activity after PR or SμG alone was lower than that obtained with the non-treatment control. No difference in cell viability or ALP activity was found between 1 Gy PR combined with SμG (PR-SμG) and PR alone. However, 4 Gy PR-SμG resulted in decreased cell viability and ALP activity compared with those obtained with PR alone. Furthermore, Gene Ontology analysis revealed the same trend. These results revealed that PR-SμG may lead to reductions in the proliferation and differentiation capacities of cells in a dose-dependent manner. Our data provide new insights into bone-related hazards caused by multiple factors, such as PR and μG, in the space environment.Liqiu MaLiqiu MaFuquan KongFuquan KongYihao GongYihao GongQiaojuan WangQiaojuan WangJiancheng LiuJiancheng LiuLi SuiLi SuiFrontiers Media S.A.articleproton radiationmicrogravitybone functiontranscriptomecombined effectsPublic aspects of medicineRA1-1270ENFrontiers in Public Health, Vol 9 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	proton radiation microgravity bone function transcriptome combined effects Public aspects of medicine RA1-1270
spellingShingle	proton radiation microgravity bone function transcriptome combined effects Public aspects of medicine RA1-1270 Liqiu Ma Liqiu Ma Fuquan Kong Fuquan Kong Yihao Gong Yihao Gong Qiaojuan Wang Qiaojuan Wang Jiancheng Liu Jiancheng Liu Li Sui Li Sui Combined Effects of Proton Radiation and Simulated Microgravity on the Cell Viability and ALP Activity of Murine Osteoblast Cells
description	Proton radiation (PR) and microgravity (μG) are two key factors that impact living things in space. This study aimed to explore the combined effects of PR and simulated μG (SμG) on bone function. Mouse embryo osteoblast precursor cells (MC3T3-E1) were irradiated with proton beams and immediately treated with SμG for 2 days using a three-dimensional clinostat. All samples were subjected to cell viability, alkaline phosphatase (ALP) activity and transcriptome assays. The results showed that cell viability decreased with increasing doses of PR. The peak ALP activity after PR or SμG alone was lower than that obtained with the non-treatment control. No difference in cell viability or ALP activity was found between 1 Gy PR combined with SμG (PR-SμG) and PR alone. However, 4 Gy PR-SμG resulted in decreased cell viability and ALP activity compared with those obtained with PR alone. Furthermore, Gene Ontology analysis revealed the same trend. These results revealed that PR-SμG may lead to reductions in the proliferation and differentiation capacities of cells in a dose-dependent manner. Our data provide new insights into bone-related hazards caused by multiple factors, such as PR and μG, in the space environment.
format	article
author	Liqiu Ma Liqiu Ma Fuquan Kong Fuquan Kong Yihao Gong Yihao Gong Qiaojuan Wang Qiaojuan Wang Jiancheng Liu Jiancheng Liu Li Sui Li Sui
author_facet	Liqiu Ma Liqiu Ma Fuquan Kong Fuquan Kong Yihao Gong Yihao Gong Qiaojuan Wang Qiaojuan Wang Jiancheng Liu Jiancheng Liu Li Sui Li Sui
author_sort	Liqiu Ma
title	Combined Effects of Proton Radiation and Simulated Microgravity on the Cell Viability and ALP Activity of Murine Osteoblast Cells
title_short	Combined Effects of Proton Radiation and Simulated Microgravity on the Cell Viability and ALP Activity of Murine Osteoblast Cells
title_full	Combined Effects of Proton Radiation and Simulated Microgravity on the Cell Viability and ALP Activity of Murine Osteoblast Cells
title_fullStr	Combined Effects of Proton Radiation and Simulated Microgravity on the Cell Viability and ALP Activity of Murine Osteoblast Cells
title_full_unstemmed	Combined Effects of Proton Radiation and Simulated Microgravity on the Cell Viability and ALP Activity of Murine Osteoblast Cells
title_sort	combined effects of proton radiation and simulated microgravity on the cell viability and alp activity of murine osteoblast cells
publisher	Frontiers Media S.A.
publishDate	2021
url	https://doaj.org/article/aa3ea4a2dea942fdb7d9075c92084afc
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Combined Effects of Proton Radiation and Simulated Microgravity on the Cell Viability and ALP Activity of Murine Osteoblast Cells

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