Neutrophil-derived miR-223 as local biomarker of bacterial peritonitis

Abstract Infection remains a major cause of morbidity, mortality and technique failure in patients with end stage kidney failure who receive peritoneal dialysis (PD). Recent research suggests that the early inflammatory response at the site of infection carries diagnostically relevant information, s...

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Autores principales:	Amy C. Brook, Robert H. Jenkins, Aled Clayton, Ann Kift-Morgan, Anne-Catherine Raby, Alex P. Shephard, Barbara Mariotti, Simone M. Cuff, Flavia Bazzoni, Timothy Bowen, Donald J. Fraser, Matthias Eberl
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2019
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/aa42ce379e3041e1bb9a6b1736dfbe8e
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spelling	oai:doaj.org-article:aa42ce379e3041e1bb9a6b1736dfbe8e2021-12-02T15:08:08ZNeutrophil-derived miR-223 as local biomarker of bacterial peritonitis10.1038/s41598-019-46585-y2045-2322https://doaj.org/article/aa42ce379e3041e1bb9a6b1736dfbe8e2019-07-01T00:00:00Zhttps://doi.org/10.1038/s41598-019-46585-yhttps://doaj.org/toc/2045-2322Abstract Infection remains a major cause of morbidity, mortality and technique failure in patients with end stage kidney failure who receive peritoneal dialysis (PD). Recent research suggests that the early inflammatory response at the site of infection carries diagnostically relevant information, suggesting that organ and pathogen-specific “immune fingerprints” may guide targeted treatment decisions and allow patient stratification and risk prediction at the point of care. Here, we recorded microRNA profiles in the PD effluent of patients presenting with symptoms of acute peritonitis and show that elevated peritoneal miR-223 and reduced miR-31 levels were useful predictors of bacterial infection. Cell culture experiments indicated that miR-223 was predominantly produced by infiltrating immune cells (neutrophils, monocytes), while miR-31 was mainly derived from the local tissue (mesothelial cells, fibroblasts). miR-223 was found to be functionally stabilised in PD effluent from peritonitis patients, with a proportion likely to be incorporated into neutrophil-derived exosomes. Our study demonstrates that microRNAs are useful biomarkers of bacterial infection in PD-related peritonitis and have the potential to contribute to disease-specific immune fingerprints. Exosome-encapsulated microRNAs may have a functional role in intercellular communication between immune cells responding to the infection and the local tissue, to help clear the infection, resolve the inflammation and restore homeostasis.Amy C. BrookRobert H. JenkinsAled ClaytonAnn Kift-MorganAnne-Catherine RabyAlex P. ShephardBarbara MariottiSimone M. CuffFlavia BazzoniTimothy BowenDonald J. FraserMatthias EberlNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 9, Iss 1, Pp 1-12 (2019)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Amy C. Brook Robert H. Jenkins Aled Clayton Ann Kift-Morgan Anne-Catherine Raby Alex P. Shephard Barbara Mariotti Simone M. Cuff Flavia Bazzoni Timothy Bowen Donald J. Fraser Matthias Eberl Neutrophil-derived miR-223 as local biomarker of bacterial peritonitis
description	Abstract Infection remains a major cause of morbidity, mortality and technique failure in patients with end stage kidney failure who receive peritoneal dialysis (PD). Recent research suggests that the early inflammatory response at the site of infection carries diagnostically relevant information, suggesting that organ and pathogen-specific “immune fingerprints” may guide targeted treatment decisions and allow patient stratification and risk prediction at the point of care. Here, we recorded microRNA profiles in the PD effluent of patients presenting with symptoms of acute peritonitis and show that elevated peritoneal miR-223 and reduced miR-31 levels were useful predictors of bacterial infection. Cell culture experiments indicated that miR-223 was predominantly produced by infiltrating immune cells (neutrophils, monocytes), while miR-31 was mainly derived from the local tissue (mesothelial cells, fibroblasts). miR-223 was found to be functionally stabilised in PD effluent from peritonitis patients, with a proportion likely to be incorporated into neutrophil-derived exosomes. Our study demonstrates that microRNAs are useful biomarkers of bacterial infection in PD-related peritonitis and have the potential to contribute to disease-specific immune fingerprints. Exosome-encapsulated microRNAs may have a functional role in intercellular communication between immune cells responding to the infection and the local tissue, to help clear the infection, resolve the inflammation and restore homeostasis.
format	article
author	Amy C. Brook Robert H. Jenkins Aled Clayton Ann Kift-Morgan Anne-Catherine Raby Alex P. Shephard Barbara Mariotti Simone M. Cuff Flavia Bazzoni Timothy Bowen Donald J. Fraser Matthias Eberl
author_facet	Amy C. Brook Robert H. Jenkins Aled Clayton Ann Kift-Morgan Anne-Catherine Raby Alex P. Shephard Barbara Mariotti Simone M. Cuff Flavia Bazzoni Timothy Bowen Donald J. Fraser Matthias Eberl
author_sort	Amy C. Brook
title	Neutrophil-derived miR-223 as local biomarker of bacterial peritonitis
title_short	Neutrophil-derived miR-223 as local biomarker of bacterial peritonitis
title_full	Neutrophil-derived miR-223 as local biomarker of bacterial peritonitis
title_fullStr	Neutrophil-derived miR-223 as local biomarker of bacterial peritonitis
title_full_unstemmed	Neutrophil-derived miR-223 as local biomarker of bacterial peritonitis
title_sort	neutrophil-derived mir-223 as local biomarker of bacterial peritonitis
publisher	Nature Portfolio
publishDate	2019
url	https://doaj.org/article/aa42ce379e3041e1bb9a6b1736dfbe8e
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Neutrophil-derived miR-223 as local biomarker of bacterial peritonitis

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