Insights into how environment shapes post-mortem RNA transcription in mouse brain

Abstract Most biological features that occur on the body after death were already deciphered by traditional medicine. However, the molecular mechanisms triggered in the cellular microenvironment are not fully comprehended yet. Previous studies reported gene expression alterations in the post-mortem...

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Autores principales:	Raphael Severino Bonadio, Larissa Barbosa Nunes, Patricia Natália S. Moretti, Juliana Forte Mazzeu, Stefano Cagnin, Aline Pic-Taylor, Silviene Fabiana de Oliveira
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/aa4a2f0180414d109838050b6d288e78
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spelling	oai:doaj.org-article:aa4a2f0180414d109838050b6d288e782021-12-02T18:02:44ZInsights into how environment shapes post-mortem RNA transcription in mouse brain10.1038/s41598-021-92268-y2045-2322https://doaj.org/article/aa4a2f0180414d109838050b6d288e782021-06-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-92268-yhttps://doaj.org/toc/2045-2322Abstract Most biological features that occur on the body after death were already deciphered by traditional medicine. However, the molecular mechanisms triggered in the cellular microenvironment are not fully comprehended yet. Previous studies reported gene expression alterations in the post-mortem condition, but little is known about how the environment could influence RNA degradation and transcriptional regulation. In this work, we analysed the transcriptome of mouse brain after death under three concealment simulations (air exposed, buried, and submerged). Our analyses identified 2,103 genes differentially expressed in all tested groups 48 h after death. Moreover, we identified 111 commonly upregulated and 497 commonly downregulated genes in mice from the concealment simulations. The gene functions shared by the individuals from the tested environments were associated with RNA homeostasis, inflammation, developmental processes, cell communication, cell proliferation, and lipid metabolism. Regarding the altered biological processes, we identified that the macroautophagy process was enriched in the upregulated genes and lipid metabolism was enriched in the downregulated genes. On the other hand, we also described a list of biomarkers associated with the submerged and buried groups, indicating that these environments can influence the post-mortem RNA abundance in its particular way.Raphael Severino BonadioLarissa Barbosa NunesPatricia Natália S. MorettiJuliana Forte MazzeuStefano CagninAline Pic-TaylorSilviene Fabiana de OliveiraNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-9 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Raphael Severino Bonadio Larissa Barbosa Nunes Patricia Natália S. Moretti Juliana Forte Mazzeu Stefano Cagnin Aline Pic-Taylor Silviene Fabiana de Oliveira Insights into how environment shapes post-mortem RNA transcription in mouse brain
description	Abstract Most biological features that occur on the body after death were already deciphered by traditional medicine. However, the molecular mechanisms triggered in the cellular microenvironment are not fully comprehended yet. Previous studies reported gene expression alterations in the post-mortem condition, but little is known about how the environment could influence RNA degradation and transcriptional regulation. In this work, we analysed the transcriptome of mouse brain after death under three concealment simulations (air exposed, buried, and submerged). Our analyses identified 2,103 genes differentially expressed in all tested groups 48 h after death. Moreover, we identified 111 commonly upregulated and 497 commonly downregulated genes in mice from the concealment simulations. The gene functions shared by the individuals from the tested environments were associated with RNA homeostasis, inflammation, developmental processes, cell communication, cell proliferation, and lipid metabolism. Regarding the altered biological processes, we identified that the macroautophagy process was enriched in the upregulated genes and lipid metabolism was enriched in the downregulated genes. On the other hand, we also described a list of biomarkers associated with the submerged and buried groups, indicating that these environments can influence the post-mortem RNA abundance in its particular way.
format	article
author	Raphael Severino Bonadio Larissa Barbosa Nunes Patricia Natália S. Moretti Juliana Forte Mazzeu Stefano Cagnin Aline Pic-Taylor Silviene Fabiana de Oliveira
author_facet	Raphael Severino Bonadio Larissa Barbosa Nunes Patricia Natália S. Moretti Juliana Forte Mazzeu Stefano Cagnin Aline Pic-Taylor Silviene Fabiana de Oliveira
author_sort	Raphael Severino Bonadio
title	Insights into how environment shapes post-mortem RNA transcription in mouse brain
title_short	Insights into how environment shapes post-mortem RNA transcription in mouse brain
title_full	Insights into how environment shapes post-mortem RNA transcription in mouse brain
title_fullStr	Insights into how environment shapes post-mortem RNA transcription in mouse brain
title_full_unstemmed	Insights into how environment shapes post-mortem RNA transcription in mouse brain
title_sort	insights into how environment shapes post-mortem rna transcription in mouse brain
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/aa4a2f0180414d109838050b6d288e78
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Insights into how environment shapes post-mortem RNA transcription in mouse brain

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