Contrasting Roles of Cannabidiol as an Insecticide and Rescuing Agent for Ethanol–induced Death in the Tobacco Hornworm Manduca sexta

Abstract Cannabis sativa, also known as marijuana or hemp, produces a non-psychoactive compound cannabidiol (CBD). To investigate the defensive role of CBD, a feeding preference assay was performed with tobacco hornworm Manduca sexta. The larvae clearly show feeding preference towards the Cannabis t...

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Autores principales:	Sang-Hyuck Park, S. Kyle Staples, Eric L. Gostin, Jeffrey P. Smith, Jose J. Vigil, Dustin Seifried, Chad Kinney, Christopher S. Pauli, Brian D. Vanden Heuvel
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2019
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/aa4cf9e7ce3a42c692ed79ad7282f5a8
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spelling	oai:doaj.org-article:aa4cf9e7ce3a42c692ed79ad7282f5a82021-12-02T15:09:13ZContrasting Roles of Cannabidiol as an Insecticide and Rescuing Agent for Ethanol–induced Death in the Tobacco Hornworm Manduca sexta10.1038/s41598-019-47017-72045-2322https://doaj.org/article/aa4cf9e7ce3a42c692ed79ad7282f5a82019-07-01T00:00:00Zhttps://doi.org/10.1038/s41598-019-47017-7https://doaj.org/toc/2045-2322Abstract Cannabis sativa, also known as marijuana or hemp, produces a non-psychoactive compound cannabidiol (CBD). To investigate the defensive role of CBD, a feeding preference assay was performed with tobacco hornworm Manduca sexta. The larvae clearly show feeding preference towards the Cannabis tissue containing low CBD over high CBD. While the larva avoided the high CBD diet, we investigated detrimental effects of CBD in the insects’ diet. Contrasted to the performance on low CBD-infused artificial diet (AD), larvae reared on the high CBD diet suffer significantly reduced growth and increased mortality. Through testing different carriers, we found that the increase of EtOH in the diet is negatively correlated with insect development and behaviors. Notably, CBD treatment significantly improved ethanol-intoxicated larval survival rate by 40% and also improved diet searching activity, resulting in increased diet consumption. Electrophysiology results revealed that the CBD-treated ganglia had delayed but much larger response with electric stimuli in comparison to the larvae reared on AD only and EtOH-added diet. Our results show CBDs’ defensive role against pest insects, which suggests its possible use as an insecticide. We also provide evidence that CBD alleviates alcohol-induced stress; consequently, improving the performance and viability of M. sexta larvae.Sang-Hyuck ParkS. Kyle StaplesEric L. GostinJeffrey P. SmithJose J. VigilDustin SeifriedChad KinneyChristopher S. PauliBrian D. Vanden HeuvelNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 9, Iss 1, Pp 1-10 (2019)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Sang-Hyuck Park S. Kyle Staples Eric L. Gostin Jeffrey P. Smith Jose J. Vigil Dustin Seifried Chad Kinney Christopher S. Pauli Brian D. Vanden Heuvel Contrasting Roles of Cannabidiol as an Insecticide and Rescuing Agent for Ethanol–induced Death in the Tobacco Hornworm Manduca sexta
description	Abstract Cannabis sativa, also known as marijuana or hemp, produces a non-psychoactive compound cannabidiol (CBD). To investigate the defensive role of CBD, a feeding preference assay was performed with tobacco hornworm Manduca sexta. The larvae clearly show feeding preference towards the Cannabis tissue containing low CBD over high CBD. While the larva avoided the high CBD diet, we investigated detrimental effects of CBD in the insects’ diet. Contrasted to the performance on low CBD-infused artificial diet (AD), larvae reared on the high CBD diet suffer significantly reduced growth and increased mortality. Through testing different carriers, we found that the increase of EtOH in the diet is negatively correlated with insect development and behaviors. Notably, CBD treatment significantly improved ethanol-intoxicated larval survival rate by 40% and also improved diet searching activity, resulting in increased diet consumption. Electrophysiology results revealed that the CBD-treated ganglia had delayed but much larger response with electric stimuli in comparison to the larvae reared on AD only and EtOH-added diet. Our results show CBDs’ defensive role against pest insects, which suggests its possible use as an insecticide. We also provide evidence that CBD alleviates alcohol-induced stress; consequently, improving the performance and viability of M. sexta larvae.
format	article
author	Sang-Hyuck Park S. Kyle Staples Eric L. Gostin Jeffrey P. Smith Jose J. Vigil Dustin Seifried Chad Kinney Christopher S. Pauli Brian D. Vanden Heuvel
author_facet	Sang-Hyuck Park S. Kyle Staples Eric L. Gostin Jeffrey P. Smith Jose J. Vigil Dustin Seifried Chad Kinney Christopher S. Pauli Brian D. Vanden Heuvel
author_sort	Sang-Hyuck Park
title	Contrasting Roles of Cannabidiol as an Insecticide and Rescuing Agent for Ethanol–induced Death in the Tobacco Hornworm Manduca sexta
title_short	Contrasting Roles of Cannabidiol as an Insecticide and Rescuing Agent for Ethanol–induced Death in the Tobacco Hornworm Manduca sexta
title_full	Contrasting Roles of Cannabidiol as an Insecticide and Rescuing Agent for Ethanol–induced Death in the Tobacco Hornworm Manduca sexta
title_fullStr	Contrasting Roles of Cannabidiol as an Insecticide and Rescuing Agent for Ethanol–induced Death in the Tobacco Hornworm Manduca sexta
title_full_unstemmed	Contrasting Roles of Cannabidiol as an Insecticide and Rescuing Agent for Ethanol–induced Death in the Tobacco Hornworm Manduca sexta
title_sort	contrasting roles of cannabidiol as an insecticide and rescuing agent for ethanol–induced death in the tobacco hornworm manduca sexta
publisher	Nature Portfolio
publishDate	2019
url	https://doaj.org/article/aa4cf9e7ce3a42c692ed79ad7282f5a8
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Contrasting Roles of Cannabidiol as an Insecticide and Rescuing Agent for Ethanol–induced Death in the Tobacco Hornworm Manduca sexta

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