The Practicability of the Xpert HCV Viral Load Fingerstick Point-of-Care Assay in Primary Care Settings

Linkage to care presents one obstacle toward eliminating HCV, and the current two-step pathway (anti-HCV, followed by HCV-RNA testing) results in the loss of patients. HCV screening was tested in the primary care setting with the fingerstick Xpert HCV viral load point-of-care assay to analyze the pr...

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Autores principales:	David Petroff, Olaf Bätz, Katrin Jedrysiak, Jan Kramer, Thomas Berg, Johannes Wiegand
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	hepatitis C screening elimination World Health Organization check-up Microbiology QR1-502
Acceso en línea:	https://doaj.org/article/aa50216420354fc7959c8af6e466b524
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spelling	oai:doaj.org-article:aa50216420354fc7959c8af6e466b5242021-11-25T19:14:36ZThe Practicability of the Xpert HCV Viral Load Fingerstick Point-of-Care Assay in Primary Care Settings10.3390/v131123271999-4915https://doaj.org/article/aa50216420354fc7959c8af6e466b5242021-11-01T00:00:00Zhttps://www.mdpi.com/1999-4915/13/11/2327https://doaj.org/toc/1999-4915Linkage to care presents one obstacle toward eliminating HCV, and the current two-step pathway (anti-HCV, followed by HCV-RNA testing) results in the loss of patients. HCV screening was tested in the primary care setting with the fingerstick Xpert HCV viral load point-of-care assay to analyze the practicability of immediate diagnosis. Anti-HCV (Cobas) and HCV-RNA (Cobas Amplicor version 2.0, only performed if anti-HCV was positive) were analyzed centrally as the gold standard. The Xpert assay was performed by 10 primary care private practices. In total, 622 patients were recruited. Five individuals (0.8%) were anti-HCV positive, and one was HCV-RNA positive. The Xpert test was valid in 546/622 (87.8%) patients. It was negative in 544 and positive in 2 cases, both of whom were anti-HCV negative. The HCV-RNA PCR and the Xpert test were both negative in 4/5 anti-HCV-positive cases, and the individual with HCV-RNA 4.5 × 10<sup>6</sup> IU/mL was not detected by the Xpert test. Primary care physicians rated the Xpert test practicability as bad, satisfactory, or good in 6%, 13%, and 81%, respectively, though 14/29 (48%) bad test ratings were assigned by a single practice. Despite adequate acceptance, interpretability and diagnostic performance in primary care settings should be further evaluated before its use in HCV screening can be recommended.David PetroffOlaf BätzKatrin JedrysiakJan KramerThomas BergJohannes WiegandMDPI AGarticlehepatitis CscreeningeliminationWorld Health Organizationcheck-upMicrobiologyQR1-502ENViruses, Vol 13, Iss 2327, p 2327 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	hepatitis C screening elimination World Health Organization check-up Microbiology QR1-502
spellingShingle	hepatitis C screening elimination World Health Organization check-up Microbiology QR1-502 David Petroff Olaf Bätz Katrin Jedrysiak Jan Kramer Thomas Berg Johannes Wiegand The Practicability of the Xpert HCV Viral Load Fingerstick Point-of-Care Assay in Primary Care Settings
description	Linkage to care presents one obstacle toward eliminating HCV, and the current two-step pathway (anti-HCV, followed by HCV-RNA testing) results in the loss of patients. HCV screening was tested in the primary care setting with the fingerstick Xpert HCV viral load point-of-care assay to analyze the practicability of immediate diagnosis. Anti-HCV (Cobas) and HCV-RNA (Cobas Amplicor version 2.0, only performed if anti-HCV was positive) were analyzed centrally as the gold standard. The Xpert assay was performed by 10 primary care private practices. In total, 622 patients were recruited. Five individuals (0.8%) were anti-HCV positive, and one was HCV-RNA positive. The Xpert test was valid in 546/622 (87.8%) patients. It was negative in 544 and positive in 2 cases, both of whom were anti-HCV negative. The HCV-RNA PCR and the Xpert test were both negative in 4/5 anti-HCV-positive cases, and the individual with HCV-RNA 4.5 × 10<sup>6</sup> IU/mL was not detected by the Xpert test. Primary care physicians rated the Xpert test practicability as bad, satisfactory, or good in 6%, 13%, and 81%, respectively, though 14/29 (48%) bad test ratings were assigned by a single practice. Despite adequate acceptance, interpretability and diagnostic performance in primary care settings should be further evaluated before its use in HCV screening can be recommended.
format	article
author	David Petroff Olaf Bätz Katrin Jedrysiak Jan Kramer Thomas Berg Johannes Wiegand
author_facet	David Petroff Olaf Bätz Katrin Jedrysiak Jan Kramer Thomas Berg Johannes Wiegand
author_sort	David Petroff
title	The Practicability of the Xpert HCV Viral Load Fingerstick Point-of-Care Assay in Primary Care Settings
title_short	The Practicability of the Xpert HCV Viral Load Fingerstick Point-of-Care Assay in Primary Care Settings
title_full	The Practicability of the Xpert HCV Viral Load Fingerstick Point-of-Care Assay in Primary Care Settings
title_fullStr	The Practicability of the Xpert HCV Viral Load Fingerstick Point-of-Care Assay in Primary Care Settings
title_full_unstemmed	The Practicability of the Xpert HCV Viral Load Fingerstick Point-of-Care Assay in Primary Care Settings
title_sort	practicability of the xpert hcv viral load fingerstick point-of-care assay in primary care settings
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/aa50216420354fc7959c8af6e466b524
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The Practicability of the Xpert HCV Viral Load Fingerstick Point-of-Care Assay in Primary Care Settings

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