Competitive Endogenous RNA Landscape in Epstein-Barr Virus Associated Nasopharyngeal Carcinoma

Non-coding RNAs have been shown to play important regulatory roles, notably in cancer development. In this study, we investigated the role of microRNAs and circular RNAs in Nasopharyngeal Carcinoma (NPC) by constructing a circRNA-miRNA-mRNA co-expression network and performing differential expressio...

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Autores principales:	Xiandong Lin, Steven Wang, Keyu Lin, Jingfeng Zong, Qianlan Zheng, Ying Su, Tao Huang
Formato:	article
Lenguaje:	EN
Publicado:	Frontiers Media S.A. 2021
Materias:	nasopharyngeal carcinoma (NCP) epstein-barr virus (EBV) circRNA miRNA network Biology (General) QH301-705.5
Acceso en línea:	https://doaj.org/article/aa5080d8a9ce400c9286f75407317323
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spelling	oai:doaj.org-article:aa5080d8a9ce400c9286f754073173232021-11-04T06:57:19ZCompetitive Endogenous RNA Landscape in Epstein-Barr Virus Associated Nasopharyngeal Carcinoma2296-634X10.3389/fcell.2021.782473https://doaj.org/article/aa5080d8a9ce400c9286f754073173232021-11-01T00:00:00Zhttps://www.frontiersin.org/articles/10.3389/fcell.2021.782473/fullhttps://doaj.org/toc/2296-634XNon-coding RNAs have been shown to play important regulatory roles, notably in cancer development. In this study, we investigated the role of microRNAs and circular RNAs in Nasopharyngeal Carcinoma (NPC) by constructing a circRNA-miRNA-mRNA co-expression network and performing differential expression analysis on mRNAs, miRNAs, and circRNAs. Specifically, the Epstein-Barr virus (EBV) infection has been found to be an important risk factor for NPC, and potential pathological differences may exist for EBV+ and EBV- subtypes of NPC. By comparing the expression profile of non-cancerous immortalized nasopharyngeal epithelial cell line and NPC cell lines, we identified differentially expressed coding and non-coding RNAs across three groups of comparison: cancer vs. non-cancer, EBV+ vs. EBV- NPC, and metastatic vs. non-metastatic NPC. We constructed a ceRNA network composed of mRNAs, miRNAs, and circRNAs, leveraging co-expression and miRNA target prediction tools. Within the network, we identified the regulatory ceRNAs of CDKN1B, ZNF302, ZNF268, and RPGR. These differentially expressed axis, along with other miRNA-circRNA pairs we identified through our analysis, helps elucidate the genetic and epigenetic changes central to NPC progression, and the differences between EBV+ and EBV- NPC.Xiandong LinXiandong LinSteven WangKeyu LinJingfeng ZongQianlan ZhengYing SuTao HuangFrontiers Media S.A.articlenasopharyngeal carcinoma (NCP)epstein-barr virus (EBV)circRNAmiRNAnetworkBiology (General)QH301-705.5ENFrontiers in Cell and Developmental Biology, Vol 9 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	nasopharyngeal carcinoma (NCP) epstein-barr virus (EBV) circRNA miRNA network Biology (General) QH301-705.5
spellingShingle	nasopharyngeal carcinoma (NCP) epstein-barr virus (EBV) circRNA miRNA network Biology (General) QH301-705.5 Xiandong Lin Xiandong Lin Steven Wang Keyu Lin Jingfeng Zong Qianlan Zheng Ying Su Tao Huang Competitive Endogenous RNA Landscape in Epstein-Barr Virus Associated Nasopharyngeal Carcinoma
description	Non-coding RNAs have been shown to play important regulatory roles, notably in cancer development. In this study, we investigated the role of microRNAs and circular RNAs in Nasopharyngeal Carcinoma (NPC) by constructing a circRNA-miRNA-mRNA co-expression network and performing differential expression analysis on mRNAs, miRNAs, and circRNAs. Specifically, the Epstein-Barr virus (EBV) infection has been found to be an important risk factor for NPC, and potential pathological differences may exist for EBV+ and EBV- subtypes of NPC. By comparing the expression profile of non-cancerous immortalized nasopharyngeal epithelial cell line and NPC cell lines, we identified differentially expressed coding and non-coding RNAs across three groups of comparison: cancer vs. non-cancer, EBV+ vs. EBV- NPC, and metastatic vs. non-metastatic NPC. We constructed a ceRNA network composed of mRNAs, miRNAs, and circRNAs, leveraging co-expression and miRNA target prediction tools. Within the network, we identified the regulatory ceRNAs of CDKN1B, ZNF302, ZNF268, and RPGR. These differentially expressed axis, along with other miRNA-circRNA pairs we identified through our analysis, helps elucidate the genetic and epigenetic changes central to NPC progression, and the differences between EBV+ and EBV- NPC.
format	article
author	Xiandong Lin Xiandong Lin Steven Wang Keyu Lin Jingfeng Zong Qianlan Zheng Ying Su Tao Huang
author_facet	Xiandong Lin Xiandong Lin Steven Wang Keyu Lin Jingfeng Zong Qianlan Zheng Ying Su Tao Huang
author_sort	Xiandong Lin
title	Competitive Endogenous RNA Landscape in Epstein-Barr Virus Associated Nasopharyngeal Carcinoma
title_short	Competitive Endogenous RNA Landscape in Epstein-Barr Virus Associated Nasopharyngeal Carcinoma
title_full	Competitive Endogenous RNA Landscape in Epstein-Barr Virus Associated Nasopharyngeal Carcinoma
title_fullStr	Competitive Endogenous RNA Landscape in Epstein-Barr Virus Associated Nasopharyngeal Carcinoma
title_full_unstemmed	Competitive Endogenous RNA Landscape in Epstein-Barr Virus Associated Nasopharyngeal Carcinoma
title_sort	competitive endogenous rna landscape in epstein-barr virus associated nasopharyngeal carcinoma
publisher	Frontiers Media S.A.
publishDate	2021
url	https://doaj.org/article/aa5080d8a9ce400c9286f75407317323
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Competitive Endogenous RNA Landscape in Epstein-Barr Virus Associated Nasopharyngeal Carcinoma

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