Genetic diversity and population structure of Saccharomyces cerevisiae strains isolated from different grape varieties and winemaking regions.

We herein evaluate intraspecific genetic diversity of fermentative vineyard-associated S. cerevisiae strains and evaluate relationships between grape varieties and geographical location on populational structures. From the musts obtained from 288 grape samples, collected from two wine regions (16 vi...

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Autores principales:	Dorit Schuller, Filipa Cardoso, Susana Sousa, Paula Gomes, Ana C Gomes, Manuel A S Santos, Margarida Casal
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2012
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/aa54849912dd42f9ae25fbdde9b17f3c
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spelling	oai:doaj.org-article:aa54849912dd42f9ae25fbdde9b17f3c2021-11-18T07:26:24ZGenetic diversity and population structure of Saccharomyces cerevisiae strains isolated from different grape varieties and winemaking regions.1932-620310.1371/journal.pone.0032507https://doaj.org/article/aa54849912dd42f9ae25fbdde9b17f3c2012-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/22393409/pdf/?tool=EBIhttps://doaj.org/toc/1932-6203We herein evaluate intraspecific genetic diversity of fermentative vineyard-associated S. cerevisiae strains and evaluate relationships between grape varieties and geographical location on populational structures. From the musts obtained from 288 grape samples, collected from two wine regions (16 vineyards, nine grape varieties), 94 spontaneous fermentations were concluded and 2820 yeast isolates were obtained that belonged mainly (92%) to the species S. cerevisiae. Isolates were classified in 321 strains by the use of ten microsatellite markers. A high strain diversity (8-43 strains per fermentation) was associated with high percentage (60-100%) of fermenting samples per vineyard, whereas a lower percentage of spontaneous fermentations (0-40%) corresponded to a rather low strain diversity (1-10 strains per fermentation).For the majority of the populations, observed heterozygosity (Ho) was about two to five times lower than the expected heterozygosity (He). The inferred ancestry showed a very high degree of admixture and divergence was observed between both grape variety and geographical region. Analysis of molecular variance showed that 81-93% of the total genetic variation existed within populations, while significant differentiation within the groups could be detected. Results from AMOVA analysis and clustering of allelic frequencies agree in the distinction of genetically more dispersed populations from the larger wine region compared to the less extended region. Our data show that grape variety is a driver of populational structures, because vineyards with distinct varieties harbor genetically more differentiated S. cerevisiae populations. Conversely, S. cerevisiae strains from vineyards in close proximity (5-10 km) that contain the same grape variety tend to be less divergent. Populational similarities did not correlate with the distance between vineyards of the two wine regions. Globally, our results show that populations of S. cerevisiae in vineyards may occur locally due to multi-factorial influences, one of them being the grape variety.Dorit SchullerFilipa CardosoSusana SousaPaula GomesAna C GomesManuel A S SantosMargarida CasalPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 7, Iss 2, p e32507 (2012)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Dorit Schuller Filipa Cardoso Susana Sousa Paula Gomes Ana C Gomes Manuel A S Santos Margarida Casal Genetic diversity and population structure of Saccharomyces cerevisiae strains isolated from different grape varieties and winemaking regions.
description	We herein evaluate intraspecific genetic diversity of fermentative vineyard-associated S. cerevisiae strains and evaluate relationships between grape varieties and geographical location on populational structures. From the musts obtained from 288 grape samples, collected from two wine regions (16 vineyards, nine grape varieties), 94 spontaneous fermentations were concluded and 2820 yeast isolates were obtained that belonged mainly (92%) to the species S. cerevisiae. Isolates were classified in 321 strains by the use of ten microsatellite markers. A high strain diversity (8-43 strains per fermentation) was associated with high percentage (60-100%) of fermenting samples per vineyard, whereas a lower percentage of spontaneous fermentations (0-40%) corresponded to a rather low strain diversity (1-10 strains per fermentation).For the majority of the populations, observed heterozygosity (Ho) was about two to five times lower than the expected heterozygosity (He). The inferred ancestry showed a very high degree of admixture and divergence was observed between both grape variety and geographical region. Analysis of molecular variance showed that 81-93% of the total genetic variation existed within populations, while significant differentiation within the groups could be detected. Results from AMOVA analysis and clustering of allelic frequencies agree in the distinction of genetically more dispersed populations from the larger wine region compared to the less extended region. Our data show that grape variety is a driver of populational structures, because vineyards with distinct varieties harbor genetically more differentiated S. cerevisiae populations. Conversely, S. cerevisiae strains from vineyards in close proximity (5-10 km) that contain the same grape variety tend to be less divergent. Populational similarities did not correlate with the distance between vineyards of the two wine regions. Globally, our results show that populations of S. cerevisiae in vineyards may occur locally due to multi-factorial influences, one of them being the grape variety.
format	article
author	Dorit Schuller Filipa Cardoso Susana Sousa Paula Gomes Ana C Gomes Manuel A S Santos Margarida Casal
author_facet	Dorit Schuller Filipa Cardoso Susana Sousa Paula Gomes Ana C Gomes Manuel A S Santos Margarida Casal
author_sort	Dorit Schuller
title	Genetic diversity and population structure of Saccharomyces cerevisiae strains isolated from different grape varieties and winemaking regions.
title_short	Genetic diversity and population structure of Saccharomyces cerevisiae strains isolated from different grape varieties and winemaking regions.
title_full	Genetic diversity and population structure of Saccharomyces cerevisiae strains isolated from different grape varieties and winemaking regions.
title_fullStr	Genetic diversity and population structure of Saccharomyces cerevisiae strains isolated from different grape varieties and winemaking regions.
title_full_unstemmed	Genetic diversity and population structure of Saccharomyces cerevisiae strains isolated from different grape varieties and winemaking regions.
title_sort	genetic diversity and population structure of saccharomyces cerevisiae strains isolated from different grape varieties and winemaking regions.
publisher	Public Library of Science (PLoS)
publishDate	2012
url	https://doaj.org/article/aa54849912dd42f9ae25fbdde9b17f3c
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Genetic diversity and population structure of Saccharomyces cerevisiae strains isolated from different grape varieties and winemaking regions.

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