Menstrual blood-derived stromal cells modulate functional properties of mouse and human macrophages

Abstract Menstrual blood-derived stromal cells (MenSCs) are emerging as a strong candidate for cell-based therapies due to their immunomodulatory properties. However, their direct impact on innate immune populations remains elusive. Since macrophages play a key role in the onset and development of i...

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Autores principales:	Rocío Martínez-Aguilar, Salvador Romero-Pinedo, M. José Ruiz-Magaña, Enrique G. Olivares, Carmen Ruiz-Ruiz, Ana C. Abadía-Molina
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2020
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/aa5b14e12f754e1997b00ef33afa6a19
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spelling	oai:doaj.org-article:aa5b14e12f754e1997b00ef33afa6a192021-12-02T15:10:32ZMenstrual blood-derived stromal cells modulate functional properties of mouse and human macrophages10.1038/s41598-020-78423-x2045-2322https://doaj.org/article/aa5b14e12f754e1997b00ef33afa6a192020-12-01T00:00:00Zhttps://doi.org/10.1038/s41598-020-78423-xhttps://doaj.org/toc/2045-2322Abstract Menstrual blood-derived stromal cells (MenSCs) are emerging as a strong candidate for cell-based therapies due to their immunomodulatory properties. However, their direct impact on innate immune populations remains elusive. Since macrophages play a key role in the onset and development of inflammation, understanding MenSCs implication in the functional properties of these cells is required to refine their clinical effects during the treatment of inflammatory disorders. In this study, we assessed the effects that MenSCs had on the recruitment of macrophages and other innate immune cells in two mouse models of acute inflammation, a thioglycollate (TGC)-elicited peritonitis model and a monobacterial sepsis model. We found that, in the TGC model, MenSCs injection reduced the percentage of macrophages recruited to the peritoneum and promoted the generation of peritoneal immune cell aggregates. In the sepsis model, MenSCs exacerbated infection by diminishing the recruitment of macrophages and neutrophils to the site of infection and inducing defective bacterial clearance. Additional in vitro studies confirmed that co-culture with MenSCs impaired macrophage bactericidal properties, affecting bacterial killing and the production of reactive oxygen intermediates. Our findings suggest that MenSCs modulate the macrophage population and that this modulation must be taken into consideration when it comes to future clinical applications.Rocío Martínez-AguilarSalvador Romero-PinedoM. José Ruiz-MagañaEnrique G. OlivaresCarmen Ruiz-RuizAna C. Abadía-MolinaNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 10, Iss 1, Pp 1-14 (2020)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Rocío Martínez-Aguilar Salvador Romero-Pinedo M. José Ruiz-Magaña Enrique G. Olivares Carmen Ruiz-Ruiz Ana C. Abadía-Molina Menstrual blood-derived stromal cells modulate functional properties of mouse and human macrophages
description	Abstract Menstrual blood-derived stromal cells (MenSCs) are emerging as a strong candidate for cell-based therapies due to their immunomodulatory properties. However, their direct impact on innate immune populations remains elusive. Since macrophages play a key role in the onset and development of inflammation, understanding MenSCs implication in the functional properties of these cells is required to refine their clinical effects during the treatment of inflammatory disorders. In this study, we assessed the effects that MenSCs had on the recruitment of macrophages and other innate immune cells in two mouse models of acute inflammation, a thioglycollate (TGC)-elicited peritonitis model and a monobacterial sepsis model. We found that, in the TGC model, MenSCs injection reduced the percentage of macrophages recruited to the peritoneum and promoted the generation of peritoneal immune cell aggregates. In the sepsis model, MenSCs exacerbated infection by diminishing the recruitment of macrophages and neutrophils to the site of infection and inducing defective bacterial clearance. Additional in vitro studies confirmed that co-culture with MenSCs impaired macrophage bactericidal properties, affecting bacterial killing and the production of reactive oxygen intermediates. Our findings suggest that MenSCs modulate the macrophage population and that this modulation must be taken into consideration when it comes to future clinical applications.
format	article
author	Rocío Martínez-Aguilar Salvador Romero-Pinedo M. José Ruiz-Magaña Enrique G. Olivares Carmen Ruiz-Ruiz Ana C. Abadía-Molina
author_facet	Rocío Martínez-Aguilar Salvador Romero-Pinedo M. José Ruiz-Magaña Enrique G. Olivares Carmen Ruiz-Ruiz Ana C. Abadía-Molina
author_sort	Rocío Martínez-Aguilar
title	Menstrual blood-derived stromal cells modulate functional properties of mouse and human macrophages
title_short	Menstrual blood-derived stromal cells modulate functional properties of mouse and human macrophages
title_full	Menstrual blood-derived stromal cells modulate functional properties of mouse and human macrophages
title_fullStr	Menstrual blood-derived stromal cells modulate functional properties of mouse and human macrophages
title_full_unstemmed	Menstrual blood-derived stromal cells modulate functional properties of mouse and human macrophages
title_sort	menstrual blood-derived stromal cells modulate functional properties of mouse and human macrophages
publisher	Nature Portfolio
publishDate	2020
url	https://doaj.org/article/aa5b14e12f754e1997b00ef33afa6a19
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Menstrual blood-derived stromal cells modulate functional properties of mouse and human macrophages

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