Hepatorenal protective efficacy of flavonoids from Ocimum basilicum extract in diabetic albino rats: A focus on hypoglycemic, antioxidant, anti-inflammatory and anti-apoptotic activities

Plant derived phytochemical therapy is a bright candidate for treatment of diabetes and its associated complications. Ocimum baslicum is used as an anti-diabetic traditional medicine. Hence, the present study investigated the effect of Hail Ocimum extract (HOE) and its total flavonoids (HOETF) again...

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Autores principales:	Mohamed S. Othman, Azza M. Khaled, Amal H. Al-Bagawi, Mohamed A. Fareid, Reda A. Ghany, Ola A. Habotta, Ahmed E. Abdel Moneim
Formato:	article
Lenguaje:	EN
Publicado:	Elsevier 2021
Materias:	Hail Ocimum Flavonoids Diabetes High-fat diet Hepatorenal Oxidative stress apoptosis Therapeutics. Pharmacology RM1-950
Acceso en línea:	https://doaj.org/article/aa5b56e7db734e1e92b7b5de83306fc7
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spelling	oai:doaj.org-article:aa5b56e7db734e1e92b7b5de83306fc72021-11-14T04:29:15ZHepatorenal protective efficacy of flavonoids from Ocimum basilicum extract in diabetic albino rats: A focus on hypoglycemic, antioxidant, anti-inflammatory and anti-apoptotic activities0753-332210.1016/j.biopha.2021.112287https://doaj.org/article/aa5b56e7db734e1e92b7b5de83306fc72021-12-01T00:00:00Zhttp://www.sciencedirect.com/science/article/pii/S0753332221010714https://doaj.org/toc/0753-3322Plant derived phytochemical therapy is a bright candidate for treatment of diabetes and its associated complications. Ocimum baslicum is used as an anti-diabetic traditional medicine. Hence, the present study investigated the effect of Hail Ocimum extract (HOE) and its total flavonoids (HOETF) against hepatorenal damage in experimental diabetes induced by high-fat diet (HFD) and injection of streptozotocin (STZ) in rats. Diabetic animals were co-treated daily with HOE, HOETF or metformin (MET) as a standard anti-diabetic drug for four weeks. Compared to controls, HFD/STZ-treatment lead to significant increases in fasting blood glucose, insulin and HOMA-IR levels. Furthermore, diabetic rats had elevated hepatic (ALT and ALP) and kidney functions (urea and creatinine) biomarkers together with disturbed lipid profile and decreased PPAR-γ gene expression. Higher levels of hepatic and renal LPO and NO paralleled with lower levels of GSH and activities of antioxidant enzymes (SOD, CAT, GPx and GR) after HFD/STZ treatment. Additionally, noteworthy inflammatory and apoptotic responses were evident in both organs of diabetic rats as witnessed by augmented levels of TNF-α, IL-1b and Bax levels with declined levels of Bcl-2. Moreover, histological examination of hepatic, renal and pancreatic tissues validated the biochemical findings. On contrary, co-treatment of diabetic animals with HOE or HOETF could decrease glucose and insulin levels together with improvement of lipid markers and alleviation of hepatorenal dysfunction, oxidative injury, inflammatory and apoptotic events. Conclusively, HOE or HOETF could be a promising complementary therapeutic option for the management of diabetic hepatorenal complication owing to their antioxidant, anti-inflammatory; anti-apoptotic properties.Mohamed S. OthmanAzza M. KhaledAmal H. Al-BagawiMohamed A. FareidReda A. GhanyOla A. HabottaAhmed E. Abdel MoneimElsevierarticleHail OcimumFlavonoidsDiabetesHigh-fat dietHepatorenalOxidative stress apoptosisTherapeutics. PharmacologyRM1-950ENBiomedicine & Pharmacotherapy, Vol 144, Iss , Pp 112287- (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Hail Ocimum Flavonoids Diabetes High-fat diet Hepatorenal Oxidative stress apoptosis Therapeutics. Pharmacology RM1-950
spellingShingle	Hail Ocimum Flavonoids Diabetes High-fat diet Hepatorenal Oxidative stress apoptosis Therapeutics. Pharmacology RM1-950 Mohamed S. Othman Azza M. Khaled Amal H. Al-Bagawi Mohamed A. Fareid Reda A. Ghany Ola A. Habotta Ahmed E. Abdel Moneim Hepatorenal protective efficacy of flavonoids from Ocimum basilicum extract in diabetic albino rats: A focus on hypoglycemic, antioxidant, anti-inflammatory and anti-apoptotic activities
description	Plant derived phytochemical therapy is a bright candidate for treatment of diabetes and its associated complications. Ocimum baslicum is used as an anti-diabetic traditional medicine. Hence, the present study investigated the effect of Hail Ocimum extract (HOE) and its total flavonoids (HOETF) against hepatorenal damage in experimental diabetes induced by high-fat diet (HFD) and injection of streptozotocin (STZ) in rats. Diabetic animals were co-treated daily with HOE, HOETF or metformin (MET) as a standard anti-diabetic drug for four weeks. Compared to controls, HFD/STZ-treatment lead to significant increases in fasting blood glucose, insulin and HOMA-IR levels. Furthermore, diabetic rats had elevated hepatic (ALT and ALP) and kidney functions (urea and creatinine) biomarkers together with disturbed lipid profile and decreased PPAR-γ gene expression. Higher levels of hepatic and renal LPO and NO paralleled with lower levels of GSH and activities of antioxidant enzymes (SOD, CAT, GPx and GR) after HFD/STZ treatment. Additionally, noteworthy inflammatory and apoptotic responses were evident in both organs of diabetic rats as witnessed by augmented levels of TNF-α, IL-1b and Bax levels with declined levels of Bcl-2. Moreover, histological examination of hepatic, renal and pancreatic tissues validated the biochemical findings. On contrary, co-treatment of diabetic animals with HOE or HOETF could decrease glucose and insulin levels together with improvement of lipid markers and alleviation of hepatorenal dysfunction, oxidative injury, inflammatory and apoptotic events. Conclusively, HOE or HOETF could be a promising complementary therapeutic option for the management of diabetic hepatorenal complication owing to their antioxidant, anti-inflammatory; anti-apoptotic properties.
format	article
author	Mohamed S. Othman Azza M. Khaled Amal H. Al-Bagawi Mohamed A. Fareid Reda A. Ghany Ola A. Habotta Ahmed E. Abdel Moneim
author_facet	Mohamed S. Othman Azza M. Khaled Amal H. Al-Bagawi Mohamed A. Fareid Reda A. Ghany Ola A. Habotta Ahmed E. Abdel Moneim
author_sort	Mohamed S. Othman
title	Hepatorenal protective efficacy of flavonoids from Ocimum basilicum extract in diabetic albino rats: A focus on hypoglycemic, antioxidant, anti-inflammatory and anti-apoptotic activities
title_short	Hepatorenal protective efficacy of flavonoids from Ocimum basilicum extract in diabetic albino rats: A focus on hypoglycemic, antioxidant, anti-inflammatory and anti-apoptotic activities
title_full	Hepatorenal protective efficacy of flavonoids from Ocimum basilicum extract in diabetic albino rats: A focus on hypoglycemic, antioxidant, anti-inflammatory and anti-apoptotic activities
title_fullStr	Hepatorenal protective efficacy of flavonoids from Ocimum basilicum extract in diabetic albino rats: A focus on hypoglycemic, antioxidant, anti-inflammatory and anti-apoptotic activities
title_full_unstemmed	Hepatorenal protective efficacy of flavonoids from Ocimum basilicum extract in diabetic albino rats: A focus on hypoglycemic, antioxidant, anti-inflammatory and anti-apoptotic activities
title_sort	hepatorenal protective efficacy of flavonoids from ocimum basilicum extract in diabetic albino rats: a focus on hypoglycemic, antioxidant, anti-inflammatory and anti-apoptotic activities
publisher	Elsevier
publishDate	2021
url	https://doaj.org/article/aa5b56e7db734e1e92b7b5de83306fc7
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Hepatorenal protective efficacy of flavonoids from Ocimum basilicum extract in diabetic albino rats: A focus on hypoglycemic, antioxidant, anti-inflammatory and anti-apoptotic activities

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