Bacillus thuringiensis chimeric proteins Cry1A.2 and Cry1B.2 to control soybean lepidopteran pests: New domain combinations enhance insecticidal spectrum of activity and novel receptor contributions.

Two new chimeric Bacillus thuringiensis (Bt) proteins, Cry1A.2 and Cry1B.2, were constructed using specific domains, which provide insecticidal activity against key lepidopteran soybean pests while minimizing receptor overlaps between themselves, current, and soon to be commercialized plant incorpor...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Danqi Chen, William J Moar, Agoston Jerga, Anilkumar Gowda, Jason S Milligan, Eric C Bretsynder, Timothy J Rydel, James A Baum, Altair Semeao, Xiaoran Fu, Victor Guzov, Karen Gabbert, Graham P Head, Jeffrey A Haas
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/aa5ceec009e4425b83b97151904e5165
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:aa5ceec009e4425b83b97151904e5165
record_format	dspace
spelling	oai:doaj.org-article:aa5ceec009e4425b83b97151904e51652021-11-25T06:23:31ZBacillus thuringiensis chimeric proteins Cry1A.2 and Cry1B.2 to control soybean lepidopteran pests: New domain combinations enhance insecticidal spectrum of activity and novel receptor contributions.1932-620310.1371/journal.pone.0249150https://doaj.org/article/aa5ceec009e4425b83b97151904e51652021-01-01T00:00:00Zhttps://doi.org/10.1371/journal.pone.0249150https://doaj.org/toc/1932-6203Two new chimeric Bacillus thuringiensis (Bt) proteins, Cry1A.2 and Cry1B.2, were constructed using specific domains, which provide insecticidal activity against key lepidopteran soybean pests while minimizing receptor overlaps between themselves, current, and soon to be commercialized plant incorporated protectants (PIP's) in soybean. Results from insect diet bioassays demonstrate that the recombinant Cry1A.2 and Cry1B.2 are toxic to soybean looper (SBL) Chrysodeixis includens Walker, velvetbean caterpillar (VBC) Anticarsia gemmatalis Hubner, southern armyworm (SAW) Spodoptera eridania, and black armyworm (BLAW) Spodoptera cosmioides with LC50 values < 3,448 ng/cm2. Cry1B.2 is of moderate activity with significant mortality and stunting at > 3,448 ng/cm2, while Cry1A.2 lacks toxicity against old-world bollworm (OWB) Helicoverpa armigera. Results from disabled insecticidal protein (DIP) bioassays suggest that receptor utilization of Cry1A.2 and Cry1B.2 proteins are distinct from each other and from current, and yet to be commercially available, Bt proteins in soy such as Cry1Ac, Cry1A.105, Cry1F.842, Cry2Ab2 and Vip3A. However, as Cry1A.2 contains a domain common to at least one commercial soybean Bt protein, resistance to this common domain in a current commercial soybean Bt protein could possibly confer at least partial cross resistance to Cry1A2. Therefore, Cry1A.2 and Cry1B.2 should provide two new tools for controlling many of the major soybean insect pests described above.Danqi ChenWilliam J MoarAgoston JergaAnilkumar GowdaJason S MilliganEric C BretsynderTimothy J RydelJames A BaumAltair SemeaoXiaoran FuVictor GuzovKaren GabbertGraham P HeadJeffrey A HaasPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 16, Iss 6, p e0249150 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Danqi Chen William J Moar Agoston Jerga Anilkumar Gowda Jason S Milligan Eric C Bretsynder Timothy J Rydel James A Baum Altair Semeao Xiaoran Fu Victor Guzov Karen Gabbert Graham P Head Jeffrey A Haas Bacillus thuringiensis chimeric proteins Cry1A.2 and Cry1B.2 to control soybean lepidopteran pests: New domain combinations enhance insecticidal spectrum of activity and novel receptor contributions.
description	Two new chimeric Bacillus thuringiensis (Bt) proteins, Cry1A.2 and Cry1B.2, were constructed using specific domains, which provide insecticidal activity against key lepidopteran soybean pests while minimizing receptor overlaps between themselves, current, and soon to be commercialized plant incorporated protectants (PIP's) in soybean. Results from insect diet bioassays demonstrate that the recombinant Cry1A.2 and Cry1B.2 are toxic to soybean looper (SBL) Chrysodeixis includens Walker, velvetbean caterpillar (VBC) Anticarsia gemmatalis Hubner, southern armyworm (SAW) Spodoptera eridania, and black armyworm (BLAW) Spodoptera cosmioides with LC50 values < 3,448 ng/cm2. Cry1B.2 is of moderate activity with significant mortality and stunting at > 3,448 ng/cm2, while Cry1A.2 lacks toxicity against old-world bollworm (OWB) Helicoverpa armigera. Results from disabled insecticidal protein (DIP) bioassays suggest that receptor utilization of Cry1A.2 and Cry1B.2 proteins are distinct from each other and from current, and yet to be commercially available, Bt proteins in soy such as Cry1Ac, Cry1A.105, Cry1F.842, Cry2Ab2 and Vip3A. However, as Cry1A.2 contains a domain common to at least one commercial soybean Bt protein, resistance to this common domain in a current commercial soybean Bt protein could possibly confer at least partial cross resistance to Cry1A2. Therefore, Cry1A.2 and Cry1B.2 should provide two new tools for controlling many of the major soybean insect pests described above.
format	article
author	Danqi Chen William J Moar Agoston Jerga Anilkumar Gowda Jason S Milligan Eric C Bretsynder Timothy J Rydel James A Baum Altair Semeao Xiaoran Fu Victor Guzov Karen Gabbert Graham P Head Jeffrey A Haas
author_facet	Danqi Chen William J Moar Agoston Jerga Anilkumar Gowda Jason S Milligan Eric C Bretsynder Timothy J Rydel James A Baum Altair Semeao Xiaoran Fu Victor Guzov Karen Gabbert Graham P Head Jeffrey A Haas
author_sort	Danqi Chen
title	Bacillus thuringiensis chimeric proteins Cry1A.2 and Cry1B.2 to control soybean lepidopteran pests: New domain combinations enhance insecticidal spectrum of activity and novel receptor contributions.
title_short	Bacillus thuringiensis chimeric proteins Cry1A.2 and Cry1B.2 to control soybean lepidopteran pests: New domain combinations enhance insecticidal spectrum of activity and novel receptor contributions.
title_full	Bacillus thuringiensis chimeric proteins Cry1A.2 and Cry1B.2 to control soybean lepidopteran pests: New domain combinations enhance insecticidal spectrum of activity and novel receptor contributions.
title_fullStr	Bacillus thuringiensis chimeric proteins Cry1A.2 and Cry1B.2 to control soybean lepidopteran pests: New domain combinations enhance insecticidal spectrum of activity and novel receptor contributions.
title_full_unstemmed	Bacillus thuringiensis chimeric proteins Cry1A.2 and Cry1B.2 to control soybean lepidopteran pests: New domain combinations enhance insecticidal spectrum of activity and novel receptor contributions.
title_sort	bacillus thuringiensis chimeric proteins cry1a.2 and cry1b.2 to control soybean lepidopteran pests: new domain combinations enhance insecticidal spectrum of activity and novel receptor contributions.
publisher	Public Library of Science (PLoS)
publishDate	2021
url	https://doaj.org/article/aa5ceec009e4425b83b97151904e5165
work_keys_str_mv	AT danqichen bacillusthuringiensischimericproteinscry1a2andcry1b2tocontrolsoybeanlepidopteranpestsnewdomaincombinationsenhanceinsecticidalspectrumofactivityandnovelreceptorcontributions AT williamjmoar bacillusthuringiensischimericproteinscry1a2andcry1b2tocontrolsoybeanlepidopteranpestsnewdomaincombinationsenhanceinsecticidalspectrumofactivityandnovelreceptorcontributions AT agostonjerga bacillusthuringiensischimericproteinscry1a2andcry1b2tocontrolsoybeanlepidopteranpestsnewdomaincombinationsenhanceinsecticidalspectrumofactivityandnovelreceptorcontributions AT anilkumargowda bacillusthuringiensischimericproteinscry1a2andcry1b2tocontrolsoybeanlepidopteranpestsnewdomaincombinationsenhanceinsecticidalspectrumofactivityandnovelreceptorcontributions AT jasonsmilligan bacillusthuringiensischimericproteinscry1a2andcry1b2tocontrolsoybeanlepidopteranpestsnewdomaincombinationsenhanceinsecticidalspectrumofactivityandnovelreceptorcontributions AT ericcbretsynder bacillusthuringiensischimericproteinscry1a2andcry1b2tocontrolsoybeanlepidopteranpestsnewdomaincombinationsenhanceinsecticidalspectrumofactivityandnovelreceptorcontributions AT timothyjrydel bacillusthuringiensischimericproteinscry1a2andcry1b2tocontrolsoybeanlepidopteranpestsnewdomaincombinationsenhanceinsecticidalspectrumofactivityandnovelreceptorcontributions AT jamesabaum bacillusthuringiensischimericproteinscry1a2andcry1b2tocontrolsoybeanlepidopteranpestsnewdomaincombinationsenhanceinsecticidalspectrumofactivityandnovelreceptorcontributions AT altairsemeao bacillusthuringiensischimericproteinscry1a2andcry1b2tocontrolsoybeanlepidopteranpestsnewdomaincombinationsenhanceinsecticidalspectrumofactivityandnovelreceptorcontributions AT xiaoranfu bacillusthuringiensischimericproteinscry1a2andcry1b2tocontrolsoybeanlepidopteranpestsnewdomaincombinationsenhanceinsecticidalspectrumofactivityandnovelreceptorcontributions AT victorguzov bacillusthuringiensischimericproteinscry1a2andcry1b2tocontrolsoybeanlepidopteranpestsnewdomaincombinationsenhanceinsecticidalspectrumofactivityandnovelreceptorcontributions AT karengabbert bacillusthuringiensischimericproteinscry1a2andcry1b2tocontrolsoybeanlepidopteranpestsnewdomaincombinationsenhanceinsecticidalspectrumofactivityandnovelreceptorcontributions AT grahamphead bacillusthuringiensischimericproteinscry1a2andcry1b2tocontrolsoybeanlepidopteranpestsnewdomaincombinationsenhanceinsecticidalspectrumofactivityandnovelreceptorcontributions AT jeffreyahaas bacillusthuringiensischimericproteinscry1a2andcry1b2tocontrolsoybeanlepidopteranpestsnewdomaincombinationsenhanceinsecticidalspectrumofactivityandnovelreceptorcontributions
_version_	1718413820178726912

Bacillus thuringiensis chimeric proteins Cry1A.2 and Cry1B.2 to control soybean lepidopteran pests: New domain combinations enhance insecticidal spectrum of activity and novel receptor contributions.

Ejemplares similares