The synergistic effect and mechanism of doxorubicin-ZnO nanocomplexes as a multimodal agent integrating diverse anticancer therapeutics

Yuxia Deng, Haijun ZhangDepartment of Oncology, Zhongda Hospital, Medical School, Southeast University, Nanjing, People's Republic of ChinaBackground: Nanomaterials have emerged as ideal multimodal nanomedicine platforms, each one combining different designs and therapeutic approaches in a s...

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Autores principales:	Deng Y, Zhang H
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2013
Materias:	Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/aa669562482c4a52a80485e4e44e5000
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spelling	oai:doaj.org-article:aa669562482c4a52a80485e4e44e50002021-12-02T00:12:16ZThe synergistic effect and mechanism of doxorubicin-ZnO nanocomplexes as a multimodal agent integrating diverse anticancer therapeutics1176-91141178-2013https://doaj.org/article/aa669562482c4a52a80485e4e44e50002013-05-01T00:00:00Zhttp://www.dovepress.com/the-synergistic-effect-and-mechanism-of-doxorubicin-zno-nanocomplexes--a12999https://doaj.org/toc/1176-9114https://doaj.org/toc/1178-2013Yuxia Deng, Haijun ZhangDepartment of Oncology, Zhongda Hospital, Medical School, Southeast University, Nanjing, People's Republic of ChinaBackground: Nanomaterials have emerged as ideal multimodal nanomedicine platforms, each one combining different designs and therapeutic approaches in a single system against cancer. The aim of the current study was to explore the synergistic effect and mechanism of a doxorubicin (Dox)-ZnO nanocomplex as a multimodal drug delivery system, integrating Dox chemotherapy and ZnO-mediated photodynamic therapy, in anticancer therapeutics.Methods: Dox was loaded onto ZnO nanomaterials, forming complexes with the transition metal Zn to yield the Dox-ZnO nanocomplexes. After culture with SMMC-7721 hepatocarcinoma cells, the cellular uptake was quantitatively detected by flow cytometry and visualized by fluorescence microscopy. The synergistic effects of the different anticancer therapeutic modalities on the proliferation of SMMC-7721 hepatocarcinoma cells were evaluated by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay. The expression of B-cell lymphoma 2 protein (Bcl-2), Bcl-2 associated X protein (Bax), caspase 9, and caspase 3 were examined by Western blot, to elucidate the possible molecular mechanisms involved.Results: Our observations demonstrated that Dox-ZnO nanocomplexes could act as an efficient drug delivery system for importing Dox into SMMC-7721 cells, enhancing its potential chemotherapy efficiency by increasing the intracellular concentration of Dox. With the addition of ultraviolet (UV) illumination, the ZnO nanomaterials showed excellent photodynamic therapeutic properties, attacking the cancer cells further. Thus the caspase-dependent apoptosis was synergistically induced, resulting in distinct improvement in anticancer activity.Conclusion: The Dox-ZnO nanocomplex presents a promising multimodal agent for comprehensive cancer treatment.Keywords: nanomedicine, nanocomplexes, drug delivery system, doxorubicin, photodynamic therapyDeng YZhang HDove Medical PressarticleMedicine (General)R5-920ENInternational Journal of Nanomedicine, Vol 2013, Iss default, Pp 1835-1841 (2013)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine (General) R5-920
spellingShingle	Medicine (General) R5-920 Deng Y Zhang H The synergistic effect and mechanism of doxorubicin-ZnO nanocomplexes as a multimodal agent integrating diverse anticancer therapeutics
description	Yuxia Deng, Haijun ZhangDepartment of Oncology, Zhongda Hospital, Medical School, Southeast University, Nanjing, People's Republic of ChinaBackground: Nanomaterials have emerged as ideal multimodal nanomedicine platforms, each one combining different designs and therapeutic approaches in a single system against cancer. The aim of the current study was to explore the synergistic effect and mechanism of a doxorubicin (Dox)-ZnO nanocomplex as a multimodal drug delivery system, integrating Dox chemotherapy and ZnO-mediated photodynamic therapy, in anticancer therapeutics.Methods: Dox was loaded onto ZnO nanomaterials, forming complexes with the transition metal Zn to yield the Dox-ZnO nanocomplexes. After culture with SMMC-7721 hepatocarcinoma cells, the cellular uptake was quantitatively detected by flow cytometry and visualized by fluorescence microscopy. The synergistic effects of the different anticancer therapeutic modalities on the proliferation of SMMC-7721 hepatocarcinoma cells were evaluated by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay. The expression of B-cell lymphoma 2 protein (Bcl-2), Bcl-2 associated X protein (Bax), caspase 9, and caspase 3 were examined by Western blot, to elucidate the possible molecular mechanisms involved.Results: Our observations demonstrated that Dox-ZnO nanocomplexes could act as an efficient drug delivery system for importing Dox into SMMC-7721 cells, enhancing its potential chemotherapy efficiency by increasing the intracellular concentration of Dox. With the addition of ultraviolet (UV) illumination, the ZnO nanomaterials showed excellent photodynamic therapeutic properties, attacking the cancer cells further. Thus the caspase-dependent apoptosis was synergistically induced, resulting in distinct improvement in anticancer activity.Conclusion: The Dox-ZnO nanocomplex presents a promising multimodal agent for comprehensive cancer treatment.Keywords: nanomedicine, nanocomplexes, drug delivery system, doxorubicin, photodynamic therapy
format	article
author	Deng Y Zhang H
author_facet	Deng Y Zhang H
author_sort	Deng Y
title	The synergistic effect and mechanism of doxorubicin-ZnO nanocomplexes as a multimodal agent integrating diverse anticancer therapeutics
title_short	The synergistic effect and mechanism of doxorubicin-ZnO nanocomplexes as a multimodal agent integrating diverse anticancer therapeutics
title_full	The synergistic effect and mechanism of doxorubicin-ZnO nanocomplexes as a multimodal agent integrating diverse anticancer therapeutics
title_fullStr	The synergistic effect and mechanism of doxorubicin-ZnO nanocomplexes as a multimodal agent integrating diverse anticancer therapeutics
title_full_unstemmed	The synergistic effect and mechanism of doxorubicin-ZnO nanocomplexes as a multimodal agent integrating diverse anticancer therapeutics
title_sort	synergistic effect and mechanism of doxorubicin-zno nanocomplexes as a multimodal agent integrating diverse anticancer therapeutics
publisher	Dove Medical Press
publishDate	2013
url	https://doaj.org/article/aa669562482c4a52a80485e4e44e5000
work_keys_str_mv	AT dengy thesynergisticeffectandmechanismofdoxorubicinznonanocomplexesasamultimodalagentintegratingdiverseanticancertherapeutics AT zhangh thesynergisticeffectandmechanismofdoxorubicinznonanocomplexesasamultimodalagentintegratingdiverseanticancertherapeutics AT dengy synergisticeffectandmechanismofdoxorubicinznonanocomplexesasamultimodalagentintegratingdiverseanticancertherapeutics AT zhangh synergisticeffectandmechanismofdoxorubicinznonanocomplexesasamultimodalagentintegratingdiverseanticancertherapeutics
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The synergistic effect and mechanism of doxorubicin-ZnO nanocomplexes as a multimodal agent integrating diverse anticancer therapeutics

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