Conservation and divergence of regulatory strategies at Hox Loci and the origin of tetrapod digits.

The evolution of tetrapod limbs from fish fins enabled the conquest of land by vertebrates and thus represents a key step in evolution. Despite the use of comparative gene expression analyses, critical aspects of this transformation remain controversial, in particular the origin of digits. Hoxa and...

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Autores principales:	Joost M Woltering, Daan Noordermeer, Marion Leleu, Denis Duboule
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2014
Materias:	Biology (General) QH301-705.5
Acceso en línea:	https://doaj.org/article/aa68190b85864131a2c4c7381176a8eb
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spelling	oai:doaj.org-article:aa68190b85864131a2c4c7381176a8eb2021-11-18T05:37:37ZConservation and divergence of regulatory strategies at Hox Loci and the origin of tetrapod digits.1544-91731545-788510.1371/journal.pbio.1001773https://doaj.org/article/aa68190b85864131a2c4c7381176a8eb2014-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/24465181/pdf/?tool=EBIhttps://doaj.org/toc/1544-9173https://doaj.org/toc/1545-7885The evolution of tetrapod limbs from fish fins enabled the conquest of land by vertebrates and thus represents a key step in evolution. Despite the use of comparative gene expression analyses, critical aspects of this transformation remain controversial, in particular the origin of digits. Hoxa and Hoxd genes are essential for the specification of the different limb segments and their functional abrogation leads to large truncations of the appendages. Here we show that the selective transcription of mouse Hoxa genes in proximal and distal limbs is related to a bimodal higher order chromatin structure, similar to that reported for Hoxd genes, thus revealing a generic regulatory strategy implemented by both gene clusters during limb development. We found the same bimodal chromatin architecture in fish embryos, indicating that the regulatory mechanism used to pattern tetrapod limbs may predate the divergence between fish and tetrapods. However, when assessed in mice, both fish regulatory landscapes triggered transcription in proximal rather than distal limb territories, supporting an evolutionary scenario whereby digits arose as tetrapod novelties through genetic retrofitting of preexisting regulatory landscapes. We discuss the possibility to consider regulatory circuitries, rather than expression patterns, as essential parameters to define evolutionary synapomorphies.Joost M WolteringDaan NoordermeerMarion LeleuDenis DuboulePublic Library of Science (PLoS)articleBiology (General)QH301-705.5ENPLoS Biology, Vol 12, Iss 1, p e1001773 (2014)
institution	DOAJ
collection	DOAJ
language	EN
topic	Biology (General) QH301-705.5
spellingShingle	Biology (General) QH301-705.5 Joost M Woltering Daan Noordermeer Marion Leleu Denis Duboule Conservation and divergence of regulatory strategies at Hox Loci and the origin of tetrapod digits.
description	The evolution of tetrapod limbs from fish fins enabled the conquest of land by vertebrates and thus represents a key step in evolution. Despite the use of comparative gene expression analyses, critical aspects of this transformation remain controversial, in particular the origin of digits. Hoxa and Hoxd genes are essential for the specification of the different limb segments and their functional abrogation leads to large truncations of the appendages. Here we show that the selective transcription of mouse Hoxa genes in proximal and distal limbs is related to a bimodal higher order chromatin structure, similar to that reported for Hoxd genes, thus revealing a generic regulatory strategy implemented by both gene clusters during limb development. We found the same bimodal chromatin architecture in fish embryos, indicating that the regulatory mechanism used to pattern tetrapod limbs may predate the divergence between fish and tetrapods. However, when assessed in mice, both fish regulatory landscapes triggered transcription in proximal rather than distal limb territories, supporting an evolutionary scenario whereby digits arose as tetrapod novelties through genetic retrofitting of preexisting regulatory landscapes. We discuss the possibility to consider regulatory circuitries, rather than expression patterns, as essential parameters to define evolutionary synapomorphies.
format	article
author	Joost M Woltering Daan Noordermeer Marion Leleu Denis Duboule
author_facet	Joost M Woltering Daan Noordermeer Marion Leleu Denis Duboule
author_sort	Joost M Woltering
title	Conservation and divergence of regulatory strategies at Hox Loci and the origin of tetrapod digits.
title_short	Conservation and divergence of regulatory strategies at Hox Loci and the origin of tetrapod digits.
title_full	Conservation and divergence of regulatory strategies at Hox Loci and the origin of tetrapod digits.
title_fullStr	Conservation and divergence of regulatory strategies at Hox Loci and the origin of tetrapod digits.
title_full_unstemmed	Conservation and divergence of regulatory strategies at Hox Loci and the origin of tetrapod digits.
title_sort	conservation and divergence of regulatory strategies at hox loci and the origin of tetrapod digits.
publisher	Public Library of Science (PLoS)
publishDate	2014
url	https://doaj.org/article/aa68190b85864131a2c4c7381176a8eb
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Conservation and divergence of regulatory strategies at Hox Loci and the origin of tetrapod digits.

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